Cytochrome C antibody - #BF0714

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产品: Cytochrome C 抗体
货号: BF0714
描述: Mouse monoclonal antibody to Cytochrome C
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat, Rabbit
分子量: 11kDa; 12kD(Calculated).
蛋白号: P99999
RRID: AB_2846192

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产品描述

来源:
Mouse
应用:
WB(1:500-1:2000), IHC(1:100-1:1000)
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Rabbit
克隆:
Monoclonal [AFB17778]
特异性:
Cytochrome C antibody detects endogenous levels of total Cytochrome C.
RRID:
AB_2846192
引用格式: Affinity Biosciences Cat# BF0714, RRID:AB_2846192.
偶联:
Unconjugated. 130
纯化:
Affinity-chromatography.
保存:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CYC; CYC_HUMAN; CYCS; Cytochrome c; Cytochrome c somatic; HCS; THC4;

抗原和靶标

免疫原:

Purified recombinant fragment of human Cytochrome C expressed in E. Coli.

Uniprot:

P99999(CYC_HUMAN) >>Visit HPA database.

基因/基因ID:
CYCS(54205)
序列:
MGDVEKGKKIFIMKCSQCHTVEKGGKHKTGPNLHGLFGRKTGQAPGYSYTAANKNKGIIWGEDTLMEYLENPKKYIPGTKMIFVGIKKKEERADLIAYLKKATNE

研究背景

功能:

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.

Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.

翻译修饰:

Binds 1 heme group per subunit.

Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.

细胞定位:

Mitochondrion intermembrane space.
Note: Loosely associated with the inner membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the cytochrome c family.

研究领域

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis - multiple species.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Metabolism > Energy metabolism > Sulfur metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Design, synthesis, and biological evaluation of 1-styrenyl isoquinoline derivatives for anti-hepatocellular carcinoma activity and effect on mitochondria. European journal of medicinal chemistry, 2023 (PubMed: 37182331) [IF=6.0]
2). Melatonin derivatives combat with inflammation-related cancer by targeting the Main Culprit STAT3. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2021 (PubMed: 33248852) [IF=6.0]

Application: WB    Species: human    Sample: HCT-116 cells

Figure 10. (A) Compound P-3 triggered the explosion of ROS in HCT-116 cells. (magnification 20 ×, scale bar = 100 μm) (B) P-3 regulated the expression of proteins involved in Cyto-c/Caspase3 pathway. Data are presented as mean ± SD from three independent experiments. * P < 0.05, ** P<0.01, *** P<0.001 versus the control group.
3). Design, synthesis and biological evaluation of N-anthraniloyl tryptamine derivatives as pleiotropic molecules for the therapy of malignant glioma. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2021 (PubMed: 34091208) [IF=6.0]

Application: WB    Species: Human    Sample: U251 and U87MG cells.

Fig. 7. NP16 induces U87MG and U251 apoptosis and ROS generation at various concentrations (1, 2 and 4 mM). (A, B): images of apoptosis-related key protein (Bax, Bcl-2 and cytochrome c) expression levels of detected by western blotting method in U251 and U87MG cells. b-actin was used as an internal loading control. (CeF) Quantification of Bcl-2/Bax ratio and cytochrome c using ImageJ software. (G) Representative DCFH-DA fluorescence images of U251 and U87MG cells treated by NP16 at 1, 2 and 4 mM (magnification,  10, scale bar ¼ 500 mm). (HeI) Reactive ROS generation ratio qualified by DCFH-DA fluorescence density in U251 and U87MG using image J. All data are expressed as the mean ± SD pooled from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 when compared with the DMSO groups treated by equivalent amounts of DMSO.

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