产品: 磷酸化 FRS2 (Tyr436) 抗体
货号: AF3466
描述: Rabbit polyclonal antibody to Phospho-FRS2 (Tyr436)
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Rat, Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 65~85kDa; 57kD(Calculated).
蛋白号: Q8WU20
RRID: AB_2834904

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货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Rat(%), Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
Phospho-FRS2 (Tyr436) Antibody detects endogenous levels of FRS2 only when phosphorylated at Tyrosine 436.
RRID:
AB_2834904
引用格式: Affinity Biosciences Cat# AF3466, RRID:AB_2834904.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FGFR signaling adaptor SNT; FGFR signalling adaptor; FGFR substrate 2; FGFR-signaling adaptor SNT; Fibroblast growth factor receptor substrate 2; FRS 2; FRS2; FRS2_HUMAN; FRS2A; FRS2alpha; SNT 1; SNT; SNT-1; SNT1; Suc 1 Associated Neurotrophic Factor Target; Suc1 associated neurotrophic factor target 1; Suc1-associated neurotrophic factor target 1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q8WU20 FRS2_HUMAN:

Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.

描述:
FRS2 is an adaptor protein involved in fibroblast growth factor receptor (FGFR) signaling. Plays an important role in linking FGFR and nerve growth factor receptors with Ras/MAPK signaling pathways.
序列:
MGSCCSCPDKDTVPDNHRNKFKVINVDDDGNELGSGIMELTDTELILYTRKRDSVKWHYLCLRRYGYDSNLFSFESGRRCQTGQGIFAFKCARAEELFNMLQEIMQNNSINVVEEPVVERNNHQTELEVPRTPRTPTTPGFAAQNLPNGYPRYPSFGDASSHPSSRHPSVGSARLPSVGEESTHPLLVAEEQVHTYVNTTGVQEERKNRTSVHVPLEARVSNAESSTPKEEPSSIEDRDPQILLEPEGVKFVLGPTPVQKQLMEKEKLEQLGRDQVSGSGANNTEWDTGYDSDERRDAPSVNKLVYENINGLSIPSASGVRRGRLTSTSTSDTQNINNSAQRRTALLNYENLPSLPPVWEARKLSRDEDDNLGPKTPSLNGYHNNLDPMHNYVNTENVTVPASAHKIEYSRRRDCTPTVFNFDIRRPSLEHRQLNYIQVDLEGGSDSDNPQTPKTPTTPLPQTPTRRTELYAVIDIERTAAMSNLQKALPRDDGTSRKTRHNSTDLPM

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8WU20 作为底物

Site PTM Type Enzyme
G2 Myristoylation
T49 Phosphorylation
Y59 Phosphorylation
S73 Phosphorylation
S76 Phosphorylation
S109 Phosphorylation
T125 Phosphorylation
T132 Phosphorylation
T135 Phosphorylation
T137 Phosphorylation
T138 Phosphorylation
Y150 Phosphorylation
Y153 Phosphorylation
S155 Phosphorylation
S169 Phosphorylation
S177 Phosphorylation
T183 Phosphorylation
Y196 Phosphorylation
T200 Phosphorylation
S211 Phosphorylation
S221 Phosphorylation
S226 Phosphorylation
T227 Phosphorylation
K267 Ubiquitination
Y290 Phosphorylation
S292 Phosphorylation
S300 Phosphorylation
Y306 Phosphorylation
S313 Phosphorylation
S316 Phosphorylation
T326 Phosphorylation
S327 Phosphorylation
T328 Phosphorylation
S329 Phosphorylation
T344 Phosphorylation
Y349 Phosphorylation
S354 Phosphorylation
S365 Phosphorylation
T376 Phosphorylation
S378 Phosphorylation
Y392 Phosphorylation
T395 Phosphorylation
S403 Phosphorylation
S428 Phosphorylation
Y436 Phosphorylation
S445 Phosphorylation
S447 Phosphorylation
T452 Phosphorylation
T455 Phosphorylation
T457 Phosphorylation
T458 Phosphorylation
T463 Phosphorylation
T465 Phosphorylation
T468 Phosphorylation
Y471 Phosphorylation
T479 Phosphorylation
S483 Phosphorylation
T499 Phosphorylation
S503 Phosphorylation
T504 Phosphorylation

研究背景

功能:

Adapter protein that links activated FGR and NGF receptors to downstream signaling pathways. Plays an important role in the activation of MAP kinases and in the phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, in response to ligand-mediated activation of FGFR1. Modulates signaling via SHC1 by competing for a common binding site on NTRK1.

翻译修饰:

Phosphorylated by ULK2 in vitro (By similarity). Phosphorylated on tyrosine residues upon stimulation by NGF or FGF2. Phosphorylated on tyrosine residues by activated ALK and FGFR1. Phosphorylated on tyrosine residues upon activation of FGFR2 and FGFR3. Phosphorylated on threonine residues by MAP kinases; this inhibits tyrosine phosphorylation, and thereby down-regulates FRS2-mediated activation of MAP kinases.

Ubiquitinated when tyrosine phosphorylated and in a complex with GRB2. The unphosphorylated form is not subject to ubiquitination (By similarity).

细胞定位:

Endomembrane system.
Note: Cytoplasmic, membrane-bound.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in heart, brain, spleen, lung, liver, skeletal muscle, kidney and testis.

亚基结构:

Part of a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Part of a complex containing GRB2 and CBL. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Binds RET (By similarity). Binds ALK, FGFR1, CKS2, MAPK1/ERK2, MAPK3/ERK1 and SRC. The tyrosine-phosphorylated protein binds the SH2 domains of GRB2 and PTPN11. Interacts with NTRK1, NTRK2 and NTRK3 (phosphorylated upon ligand-binding).

研究领域

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

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