NLRP1 Antibody - #DF13187

Figure 3. Increased BECN2 attenuated the autophagy and inflammation of LPS-induced ATDC5 degeneration. (A) Western blot was employed to detect the expression of ATG14, VPS34 and GASP1. (B) The protein expression of ATG14, VPS34 and GASP1 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). (C) ATG14, VPS34 and GASP1 expression were determined by immunofluorescence staining (scale bar: 100μm). (D–F) Average optical density was calculated by ImageJ software. (G) Western blot was employed to detect the expression of NLRP3, NLRC4, NLRP1 and AIM2. (H) The protein expression of NLRP3, NLRC4, NLRP1 and AIM2 were determined using ImageJ software, GAPDH was used as the internal control, respectively (n=3). (I) NLRP3, NLRC4, NLRP1 and AIM2 expression were determined by immunofluorescence staining (scale bar: 100μm). (J–M) Average optical density was calculated by ImageJ software. All data represent mean ± SD. All in vitro experiments were repeated three times independently. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Figure 2 GDF-11 attenuates pyroptosis following spinal cord injury. (a) Immunofluorescence staining for Caspase-1 and NeuN colocalization in the spinal cords of the GDF-11, SCI, and sham groups (scale bar = 25 μm). (b) The quantitative mean optical density of Caspase-1 in neurons of spinal cord lesions. (c) Immunofluorescence staining for GSDMD and NeuN colocalization in the spinal cords of the GDF-11, SCI, and sham groups (scale bar = 25 μm). (d) The quantitative average optical density of GSDMD within neurons of spinal cord lesions. (e) Western blot assay for IL-18, IL-1β, GSDMD, Caspase-1, ASC, NLRP3, and NLRP1 expression levels in the three groups. Gels were subjected to identical experimental conditions, with cropped blots presented. (f) Optical densities of the IL-18, IL-1β, GSDMD, Caspase-1, ASC, NLRP3, and NLRP1 expression levels were quantified and investigated in the respective groups. Data are expressed as the mean ± SEM, n = 6 per group. ∗∗p < 0.01 vs. the sham group. #p < 0.05 and ##p < 0.01 vs. the SCI group.