MFAP5 Antibody - #DF13146

Fig. 1 MFAP5 expression was associated with osteogenesis. A MFAP5 expression was lower in the BMSCs of patients with osteoporosis compared to those with osteoarthritis. B Osteogenic induction of human mesenchymal stromal cells (hMSCs) at 0, 1, 2, 3, and 4 days showed that the level of mRNA encoding MFAP5 was positively associated with osteogenesis. C The expression patterns of MFAP5 in C3H10 and MC3T3-E1 cells during osteogenic differentiation, as revealed by Western blotting and the quantitative results of Western blotting. D Mineralized nodules and ALP expression revealed by AR-S and ALP staining during the osteogenesis of C3H10 and MC3T3-E1 cells. E The relative ALP expression levels of the two cell lines. F Quantitation of AR-S staining of C3H10 and MC3T3-E1 cells. G and H The levels of mRNAs encoding MFAP5, Runx2, Col1α1, and OCN of both cell types during osteogenesis. Values are expressed as means ± standard deviations (*P 

Figure 1 Association of MFAP5 with adipogenic differentiation. (A) Fifty-seven genes were at the intersection of three sequencing datasets before and after adipogenic differentiation. (B) Radar chart of 25 genes with the highest differential expression. Blue and red, down- and up-regulated during adipogenesis, respectively. Multiples of gene expression in each database are indicated by different colors. (C) Oil red O staining of C3H10 and 3T3-L1 cells. (D) Endogenous expression and expression pattern during adipogenesis of MFAP5 in C3H10 and 3T3-L1 cells. (E–G) Relative mRNA levels of MFAP5, CEBPα, FABP4, and SREB1 during adipogenesis. n = 3, the experiment was repeated 3 times. Values are means ± SD. *P