| 产品: | INPP5B 抗体 |
| 货号: | DF13090 |
| 描述: | Rabbit polyclonal antibody to INPP5B |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Sheep |
| 分子量: | 75 kDa; 113kD(Calculated). |
| 蛋白号: | P32019 |
| RRID: | AB_2846050 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13090, RRID:AB_2846050.
展开/折叠
4; 5-trisphosphate 5-phosphatase; 5PTase; 75 kDa inositol polyphosphate 5 phosphatase; 75 kDa inositol polyphosphate-5-phosphatase; 75kDa; EC 3.1.3.36; I5P2_HUMAN; Inositol polyphosphate 5-phosphatase II; Inositol polyphosphate-5-phosphatase B; Inositol polyphosphate-5-phosphatase, 75kDa; INPP5B; Inpp5b protein; INPP5P; MGC65156; MGC71303; OTTHUMP00000004726; OTTHUMP00000004727; OTTHUMP00000004728; Phosphoinositide 5 phosphatase; Phosphoinositide 5-phosphatase; Type II inositol 1,4,5 trisphosphate 5 phosphatase; Type II inositol-1;
抗原和靶标
- P32019 I5P2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDQSVAIQETLAEGEYCVIAVQGVLCEGDSRQSRLLGLVRYRLEHGGQEHALFLYTHRRMAITGDDVSLDQIVPVSRDFTLEEVSPDGELYILGSDVTVQLDTAELSLVFQLPFGSQTRMFLHEVARACPGFDSATRDPEFLWLSRYRCAELELEMPTPRGCNSALVTWPGYATIGGGRYPSRKKRWGLEEARPQGAGSVLFWGGAMEKTGFRLMERAHGGGFVWGRSARDGRRDEELEEAGREMSAAAGSRERNTAGGSNFDGLRPNGKGVPMDQSSRGQDKPESLQPRQNKSKSEITDMVRSSTITVSDKAHILSMQKFGLRDTIVKSHLLQKEEDYTYIQNFRFFAGTYNVNGQSPKECLRLWLSNGIQAPDVYCVGFQELDLSKEAFFFHDTPKEEEWFKAVSEGLHPDAKYAKVKLIRLVGIMLLLYVKQEHAAYISEVEAETVGTGIMGRMGNKGGVAIRFQFHNTSICVVNSHLAAHIEEYERRNQDYKDICSRMQFCQPDPSLPPLTISNHDVILWLGDLNYRIEELDVEKVKKLIEEKDFQMLYAYDQLKIQVAAKTVFEGFTEGELTFQPTYKYDTGSDDWDTSEKCRAPAWCDRILWKGKNITQLSYQSHMALKTSDHKPVSSVFDIGVRVVNDELYRKTLEEIVRSLDKMENANIPSVSLSKREFCFQNVKYMQLKVESFTIHNGQVPCHFEFINKPDEESYCKQWLNANPSRGFLLPDSDVEIDLELFVNKMTATKLNSGEDKIEDILVLHLDRGKDYFLSVSGNYLPSCFGSPIHTLCYMREPILDLPLETISELTLMPVWTGDDGSQLDSPMEIPKELWMMVDYLYRNAVQQEDLFQQPGLRSEFEHIRDCLDTGMIDNLSASNHSVAEALLLFLESLPEPVICYSTYHNCLECSGNYTASKQVISTLPIFHKNVFHYLMAFLRELLKNSAKNHLDENILASIFGSLLLRNPAGHQKLDMTEKKKAQEFIHQFLCNPL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
Hydrolyzes phosphatidylinositol 4,5-bisphosphate (PtIns(4,5)P2) and the signaling molecule phosphatidylinositol 1,4,5-trisphosphate (PtIns(1,4,5)P3), and thereby modulates cellular signaling events.
Isoprenylation at Cys-990 may be required for localization at the membrane.
May be proteolytically cleaved after Lys-320 as inferred from N-terminal protein sequence of the 75 kda form.
Cytoplasm>Cytosol. Endoplasmic reticulum-Golgi intermediate compartment. Early endosome membrane. Membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle>Phagosome membrane. Golgi apparatus.
Platelets.
Interacts with APPL1, FAM109A and FAM109B. Interacts with several Rab GTPases, at least RAB1A, RAB2A, RAB5A, RAB6A, RAB8A, RAB9A and RAB33B; these interactions may play a dual role in targeting INPP5B to the specific membranes and stimulating its phosphatase activity Ref.12). Interacts preferentially with non-phosphorylated RAB8A; phosphoryation of RAB8A on 'Thr-72' disrupts this interaction. Interacts with INPP5F.
The ASH (ASPM-SPD2-Hydin) and RhoGAP (Rho GTPase activating) domains form a single folding module. The ASH domain has an immunoglobulin-like fold, the Rho-GAP domain lacks the catalytic arginine and is catalytically inactive. The ASH-RhoGAP module regulates the majority of the protein-protein interactions currently described. The ASH domain mediates association with membrane-targeting Rab GTPases. The Rho-GAP domain interacts with the endocytic adapter APPL1, which is then displaced by FAM109A and FAM109B as endosomes mature, all three interactions rely on F&H motifs, an approximately 12-13 amino-acid sequence centered around Phe and His residues essential for binding (By similarity).
Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family.
研究领域
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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