HMGCL Antibody - #DF13063

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产品: HMGCL 抗体
货号: DF13063
描述: Rabbit polyclonal antibody to HMGCL
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 34 kDa; 34kD(Calculated).
蛋白号: P35914
RRID: AB_2846024

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 50ul RMB¥ 1500 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
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实验方案
WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(88%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
HMGCL Antibody detects endogenous levels of total HMGCL.
RRID:
AB_2846024
引用格式: Affinity Biosciences Cat# DF13063, RRID:AB_2846024.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

3 hydroxy 3 methylglutaryl CoA lyase; 3 hydroxy 3 methylglutaryl Coenzyme A lyase; 3 hydroxymethyl 3 methylglutaryl Coenzyme A lyase (hydroxymethylglutaricaciduria); 3 hydroxymethyl 3 methylglutaryl Coenzyme A lyase; 3-hydroxy-3-methylglutarate-CoA lyase; HL; HMG CoA lyase; HMG CoA Lyase Deficiency; HMG-CoA lyase; HMGCL; HMGCL_HUMAN; Hydroxymethylglutaricaciduria; Hydroxymethylglutaryl CoA lyase; Hydroxymethylglutaryl CoA lyase mitochondrial; Hydroxymethylglutaryl-CoA lyase; Mitochondrial 3 hydroxy 3 methylglutaryl CoA lyase; mitochondrial; MS725; OTTHUMP00000044830;

抗原和靶标

免疫原:
Uniprot:

P35914(HMGCL_HUMAN) >>Visit HPA database.

基因/基因ID:
HMGCL(3155)
表达:
P35914 HMGCL_HUMAN:

Highest expression in liver. Expressed in pancreas, kidney, intestine, testis, fibroblasts and lymphoblasts. Very low expression in brain and skeletal muscle. The relative expression of isoform 2 (at mRNA level) is highest in heart (30%), skeletal muscle (22%), and brain (14%).

序列:
MAAMRKALPRRLVGLASLRAVSTSSMGTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKFYSMGCYEISLGDTIGVGTPGIMKDMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQATCKL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
88
Dog
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P35914 作为底物

Site PTM Type Enzyme
S17 Phosphorylation
S22 Phosphorylation
T23 Phosphorylation
K48 Acetylation
K48 Ubiquitination
S78 Phosphorylation
K80 Methylation
K93 Acetylation
K93 Ubiquitination
K137 Acetylation
Y198 Phosphorylation
C323 S-Nitrosylation
K324 Acetylation

研究背景

功能:

Mitochondrial 3-hydroxymethyl-3-methylglutaryl-CoA lyase that catalyzes a cation-dependent cleavage of (S)-3-hydroxy-3-methylglutaryl-CoA into acetyl-CoA and acetoacetate, a key step in ketogenesis. Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism.

细胞定位:

Mitochondrion matrix. Peroxisome.
Note: Unprocessed form is peroxisomal.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highest expression in liver. Expressed in pancreas, kidney, intestine, testis, fibroblasts and lymphoblasts. Very low expression in brain and skeletal muscle. The relative expression of isoform 2 (at mRNA level) is highest in heart (30%), skeletal muscle (22%), and brain (14%).

亚基结构:

Homodimer; disulfide-linked. Can also form homotetramers.

蛋白家族:

Belongs to the HMG-CoA lyase family.

研究领域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Metabolism > Lipid metabolism > Synthesis and degradation of ketone bodies.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Carbohydrate metabolism > Butanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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