产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3453, RRID:AB_2834891.
展开/折叠
hLKB1; Liver kinase B1; LKB1; PJS; Polarization related protein LKB1; Renal carcinoma antigen NY-REN-19; Serine/Threonine Kinase 11; Serine/threonine protein kinase 11; Serine/threonine protein kinase LKB1; Serine/threonine protein kinase STK11; Serine/threonine-protein kinase 11; Serine/threonine-protein kinase LKB1; Serine/threonine-protein kinase XEEK1; Stk11; STK11_HUMAN;
抗原和靶标
- Q15831 STK11_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEVVDPQQLGMFTEGELMSVGMDTFIHRIDSTEVIYQPRRKRAKLIGKYLMGDLLGEGSYGKVKEVLDSETLCRRAVKILKKKKLRRIPNGEANVKKEIQLLRRLRHKNVIQLVDVLYNEEKQKMYMVMEYCVCGMQEMLDSVPEKRFPVCQAHGYFCQLIDGLEYLHSQGIVHKDIKPGNLLLTTGGTLKISDLGVAEALHPFAADDTCRTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTGLYPFEGDNIYKLFENIGKGSYAIPGDCGPPLSDLLKGMLEYEPAKRFSIRQIRQHSWFRKKHPPAEAPVPIPPSPDTKDRWRSMTVVPYLEDLHGADEDEDLFDIEDDIIYTQDFTVPGQVPEEEASHNGQRRGLPKAVCMNGTEAAQLSTKSRAEGRAPNPARKACSASSKIRRLSACKQQ
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15831 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S31 | Phosphorylation | Uniprot | |
T32 | Phosphorylation | Uniprot | |
Y36 | Phosphorylation | P06239 (LCK) | Uniprot |
K44 | Acetylation | Uniprot | |
K48 | Acetylation | Uniprot | |
K64 | Acetylation | Uniprot | |
K96 | Acetylation | Uniprot | |
K97 | Acetylation | Uniprot | |
K178 | Sumoylation | Uniprot | |
T185 | Phosphorylation | Q15831 (STK11) | Uniprot |
T189 | Phosphorylation | Q15831 (STK11) | Uniprot |
S240 | Phosphorylation | Uniprot | |
Y261 | Phosphorylation | P06239 (LCK) , P06241 (FYN) | Uniprot |
K296 | Acetylation | Uniprot | |
S307 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
K311 | Acetylation | Uniprot | |
S325 | Phosphorylation | Uniprot | |
S334 | Phosphorylation | Uniprot | |
T336 | Phosphorylation | Q15831 (STK11) | Uniprot |
Y362 | Phosphorylation | P06241 (FYN) , P06239 (LCK) | Uniprot |
T363 | Phosphorylation | Q15831 (STK11) , P78527 (PRKDC) , Q13315 (ATM) | Uniprot |
T367 | Phosphorylation | Q13315 (ATM) | Uniprot |
S401 | Phosphorylation | Uniprot | |
T402 | Phosphorylation | Q15831 (STK11) | Uniprot |
S404 | Phosphorylation | Uniprot | |
K416 | Acetylation | Uniprot | |
K423 | Acetylation | Uniprot | |
S428 | Phosphorylation | Q9UK32 (RPS6KA6) , Q05513 (PRKCZ) , P27361 (MAPK3) , P28482 (MAPK1) , O75582 (RPS6KA5) , Q15831 (STK11) , Q15418 (RPS6KA1) , P17612 (PRKACA) | Uniprot |
K431 | Acetylation | Uniprot |
翻译修饰 - Q15831 作为激酶
Substrate | Site | Source |
---|---|---|
O60285 (NUAK1) | T211 | Uniprot |
P04637 (TP53) | S15 | Uniprot |
P04637 (TP53) | S392 | Uniprot |
P27448 (MARK3) | T211 | Uniprot |
P27448 (MARK3) | S215 | Uniprot |
P43268 (ETV4) | S396 | Uniprot |
P49841 (GSK3B) | S9 | Uniprot |
P54646 (PRKAA2) | T172 | Uniprot |
P54646 (PRKAA2) | T485 | Uniprot |
P57059 (SIK1) | T182 | Uniprot |
P60484 (PTEN) | S380 | Uniprot |
P60484 (PTEN) | T382 | Uniprot |
P60484 (PTEN) | T383 | Uniprot |
P60484 (PTEN) | S385 | Uniprot |
Q13131 (PRKAA1) | T183 | Uniprot |
Q13153 (PAK1) | T109 | Uniprot |
Q13485 (SMAD4) | T77 | Uniprot |
Q14680 (MELK) | T167 | Uniprot |
Q15831 (STK11) | T185 | Uniprot |
Q15831 (STK11) | T189 | Uniprot |
Q15831 (STK11) | T336 | Uniprot |
Q15831 (STK11) | T363 | Uniprot |
Q15831 (STK11) | T402 | Uniprot |
Q15831 (STK11) | S428 | Uniprot |
Q7KZI7 (MARK2) | T208 | Uniprot |
Q7RTN6-5 (STRADA) | T271 | Uniprot |
Q7RTN6-3 (STRADA) | T292 | Uniprot |
Q7RTN6 (STRADA) | T329 | Uniprot |
Q7RTN6-5 (STRADA) | T361 | Uniprot |
Q7RTN6-3 (STRADA) | T382 | Uniprot |
Q7RTN6-1 (STRADA) | T419 | Uniprot |
Q8IWQ3 (BRSK2) | T174 | Uniprot |
Q8TDC3 (BRSK1) | T189 | Uniprot |
Q8TDC3-2 (BRSK1) | T205 | Uniprot |
Q96L34 (MARK4) | T214 | Uniprot |
Q9H093 (NUAK2) | T208 | Uniprot |
Q9H0K1 (SIK2) | T175 | Uniprot |
Q9NRH2-1 (SNRK) | T173 | Uniprot |
Q9P0L2-1 (MARK1) | T215 | Uniprot |
Q9P0L2-1 (MARK1) | S219 | Uniprot |
研究背景
Tumor suppressor serine/threonine-protein kinase that controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. Acts by phosphorylating the T-loop of AMPK family proteins, thus promoting their activity: phosphorylates PRKAA1, PRKAA2, BRSK1, BRSK2, MARK1, MARK2, MARK3, MARK4, NUAK1, NUAK2, SIK1, SIK2, SIK3 and SNRK but not MELK. Also phosphorylates non-AMPK family proteins such as STRADA, PTEN and possibly p53/TP53. Acts as a key upstream regulator of AMPK by mediating phosphorylation and activation of AMPK catalytic subunits PRKAA1 and PRKAA2 and thereby regulates processes including: inhibition of signaling pathways that promote cell growth and proliferation when energy levels are low, glucose homeostasis in liver, activation of autophagy when cells undergo nutrient deprivation, and B-cell differentiation in the germinal center in response to DNA damage. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton. Required for cortical neuron polarization by mediating phosphorylation and activation of BRSK1 and BRSK2, leading to axon initiation and specification. Involved in DNA damage response: interacts with p53/TP53 and recruited to the CDKN1A/WAF1 promoter to participate in transcription activation. Able to phosphorylate p53/TP53; the relevance of such result in vivo is however unclear and phosphorylation may be indirect and mediated by downstream STK11/LKB1 kinase NUAK1. Also acts as a mediator of p53/TP53-dependent apoptosis via interaction with p53/TP53: translocates to the mitochondrion during apoptosis and regulates p53/TP53-dependent apoptosis pathways. In vein endothelial cells, inhibits PI3K/Akt signaling activity and thus induces apoptosis in response to the oxidant peroxynitrite (in vitro). Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with NUAK1, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair.
Has a role in spermiogenesis.
Phosphorylated by ATM at Thr-363 following ionizing radiation (IR). Phosphorylation at Ser-428 by RPS6KA1 and/or some PKA is required to inhibit cell growth. Phosphorylation at Ser-428 is also required during neuronal polarization to mediate phosphorylation of BRSK1 and BRSK2 (By similarity). Phosphorylation by PKC/PRKCZ at Ser-428 promotes peroxynitrite-induced nuclear export of STK11, leading to PTEN activation and subsequent inhibition of AKT signaling. Phosphorylation by PKC/PRKCZ at Ser-399 in isoform 2 promotes metformin (or peroxynitrite)-induced nuclear export of STK11 and activation of AMPK. UV radiation-induced phosphorylation at Thr-363 mediates CDKN1A degradation (By similarity).
Acetylated. Deacetylation at Lys-48 enhances cytoplasmic localization and kinase activity in vitro.
Nucleus. Cytoplasm. Membrane. Mitochondrion.
Note: A small fraction localizes at membranes (By similarity). Relocates to the cytoplasm when bound to STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta). Translocates to the mitochondrion during apoptosis. Translocates to the cytoplasm in response to metformin or peroxynitrite treatment. PTEN promotes cytoplasmic localization.
Nucleus. Cytoplasm.
Note: Predominantly nuclear, but translocates to the cytoplasm in response to metformin or peroxynitrite treatment.
Ubiquitously expressed. Strongest expression in testis and fetal liver.
Catalytic component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity. Found in a ternary complex composed of SMAD4, STK11/LKB1 and STK11IP. Interacts with p53/TP53, SMAD4, STK11IP and WDR6. Interacts with NR4A1. Interacts with NISCH; this interaction may increase STK11 activity. Interacts with PTEN; leading to PTEN phosphorylation. Interacts with SIRT1; the interaction deacetylates STK11. Interacts with CDKN1A.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. LKB1 subfamily.
研究领域
· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.
文献引用
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