Phospho-TRAP220/MED1 (Thr1457) Antibody - #AF3446

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产品: 磷酸化 TRAP220/MED1 (Thr1457) 抗体
货号: AF3446
描述: Rabbit polyclonal antibody to Phospho-TRAP220/MED1 (Thr1457)
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 168kDa; 168kD(Calculated).
蛋白号: Q15648
RRID: AB_2834888

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(91%)
克隆:
Polyclonal
特异性:
Phospho-TRAP220/MED1 (Thr1457) Antibody detects endogenous levels of TRAP220/MED1 only when phosphorylated at Threonine 1457.
RRID:
AB_2834888
引用格式: Affinity Biosciences Cat# AF3446, RRID:AB_2834888.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Activator-recruited cofactor 205 kDa component; ARC205; CRSP1; CRSP200; DRIP205; DRIP230; MED1; MED1_HUMAN; Mediator complex subunit 1; Mediator of RNA polymerase II transcription subunit 1; p53 regulatory protein RB18A; PBP; Peroxisome proliferator-activated receptor-binding protein; PPAR binding protein; PPAR-binding protein; PPARBP; PPARGBP; RB18A; Thyroid hormone receptor-associated protein complex 220 kDa component; Thyroid receptor-interacting protein 2; TR-interacting protein 2; Trap220; TRIP-2; TRIP2; Vitamin D receptor-interacting protein complex component DRIP205;

抗原和靶标

免疫原:
Uniprot:

Q15648(MED1_HUMAN) >>Visit HPA database.

基因/基因ID:
MED1(5469)
表达:
Q15648 MED1_HUMAN:

Ubiquitously expressed.

描述:
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1.
序列:
MKAQGETEESEKLSKMSSLLERLHAKFNQNRPWSETIKLVRQVMEKRVVMSSGGHQHLVSCLETLQKALKVTSLPAMTDRLESIARQNGLGSHLSASGTECYITSDMFYVEVQLDPAGQLCDVKVAHHGENPVSCPELVQQLREKNFDEFSKHLKGLVNLYNLPGDNKLKTKMYLALQSLEQDLSKMAIMYWKATNAGPLDKILHGSVGYLTPRSGGHLMNLKYYVSPSDLLDDKTASPIILHENNVSRSLGMNASVTIEGTSAVYKLPIAPLIMGSHPVDNKWTPSFSSITSANSVDLPACFFLKFPQPIPVSRAFVQKLQNCTGIPLFETQPTYAPLYELITQFELSKDPDPIPLNHNMRFYAALPGQQHCYFLNKDAPLPDGRSLQGTLVSKITFQHPGRVPLILNLIRHQVAYNTLIGSCVKRTILKEDSPGLLQFEVCPLSESRFSVSFQHPVNDSLVCVVMDVQDSTHVSCKLYKGLSDALICTDDFIAKVVQRCMSIPVTMRAIRRKAETIQADTPALSLIAETVEDMVKKNLPPASSPGYGMTTGNNPMSGTTTPTNTFPGGPITTLFNMSMSIKDRHESVGHGEDFSKVSQNPILTSLLQITGNGGSTIGSSPTPPHHTPPPVSSMAGNTKNHPMLMNLLKDNPAQDFSTLYGSSPLERQNSSSGSPRMEICSGSNKTKKKKSSRLPPEKPKHQTEDDFQRELFSMDVDSQNPIFDVNMTADTLDTPHITPAPSQCSTPPTTYPQPVPHPQPSIQRMVRLSSSDSIGPDVTDILSDIAEEASKLPSTSDDCPAIGTPLRDSSSSGHSQSTLFDSDVFQTNNNENPYTDPADLIADAAGSPSSDSPTNHFFHDGVDFNPDLLNSQSQSGFGEEYFDESSQSGDNDDFKGFASQALNTLGVPMLGGDNGETKFKGNNQADTVDFSIISVAGKALAPADLMEHHSGSQGPLLTTGDLGKEKTQKRVKEGNGTSNSTLSGPGLDSKPGKRSRTPSNDGKSKDKPPKRKKADTEGKSPSHSSSNRPFTPPTSTGGSKSPGSAGRSQTPPGVATPPIPKITIQIPKGTVMVGKPSSHSQYTSSGSVSSSGSKSHHSHSSSSSSSASTSGKMKSSKSEGSSSSKLSSSMYSSQGSSGSSQSKNSSQSGGKPGSSPITKHGLSSGSSSTKMKPQGKPSSLMNPSLSKPNISPSHSRPPGGSDKLASPMKPVPGTPPSSKAKSPISSGSGGSHMSGTSSSSGMKSSSGLGSSGSLSQKTPPSSNSCTASSSSFSSSGSSMSSSQNQHGSSKGKSPSRNKKPSLTAVIDKLKHGVVTSGPGGEDPLDGQMGVSTNSSSHPMSSKHNMSGGEFQGKREKSDKDKSKVSTSGSSVDSSKKTSESKNVGSTGVAKIIISKHDGGSPSIKAKVTLQKPGESSGEGLRPQMASSKNYGSPLISGSTPKHERGSPSHSKSPAYTPQNLDSESESGSSIAEKSYQNSPSSDDGIRPLPEYSTEKHKKHKKEKKKVKDKDRDRDRDKDRDKKKSHSIKPESWSKSPISSDQSLSMTSNTILSADRPSRLSPDFMIGEEDDDLMDVALIGN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Xenopus
91
Zebrafish
67
Pig
50
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15648 作为底物

Site PTM Type Enzyme
K2 Methylation
S10 Phosphorylation
K15 Acetylation
K15 Ubiquitination
S18 Phosphorylation
K26 Ubiquitination
K145 Ubiquitination
S151 Phosphorylation
K152 Methylation
K168 Ubiquitination
S179 Phosphorylation
K202 Ubiquitination
T212 Phosphorylation
Y224 Phosphorylation
Y225 Phosphorylation
K235 Ubiquitination
S238 Phosphorylation
T262 Phosphorylation
K378 Ubiquitination
K426 Ubiquitination
K431 Ubiquitination
K481 Ubiquitination
K496 Ubiquitination
S503 Phosphorylation
T507 Phosphorylation
T522 Phosphorylation
K537 Ubiquitination
S588 Phosphorylation
S596 Phosphorylation
S620 Phosphorylation
T628 Phosphorylation
S634 Phosphorylation
S663 Phosphorylation
S664 Phosphorylation
S671 Phosphorylation
S672 Phosphorylation
S673 Phosphorylation
S675 Phosphorylation
S682 Phosphorylation
S684 Phosphorylation
T687 Phosphorylation
T704 Phosphorylation O14757 (CHEK1)
S719 Phosphorylation
S770 Phosphorylation
S771 Phosphorylation
S772 Phosphorylation
S774 Phosphorylation
T780 Phosphorylation
S784 Phosphorylation
S795 Phosphorylation
T805 Phosphorylation
S816 Phosphorylation Q13315 (ATM)
S848 Phosphorylation
S850 Phosphorylation
S851 Phosphorylation
S853 Phosphorylation
S872 Phosphorylation Q13315 (ATM)
S874 Phosphorylation Q13315 (ATM)
S887 Phosphorylation Q13315 (ATM)
S900 Phosphorylation
T928 Phosphorylation
S951 Phosphorylation
S953 Phosphorylation Q13315 (ATM)
K965 Acetylation
K973 Acetylation
K991 Acetylation
K994 Acetylation
S1000 Phosphorylation
K1004 Acetylation
K1006 Methylation
K1011 Acetylation
S1021 Phosphorylation
S1023 Phosphorylation
T1032 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S1036 Phosphorylation
S1040 Phosphorylation
S1042 Phosphorylation
S1045 Phosphorylation
S1049 Phosphorylation
T1051 Phosphorylation
T1057 Phosphorylation
K1076 Acetylation
S1081 Phosphorylation
S1088 Phosphorylation
S1092 Phosphorylation
K1126 Acetylation
S1128 Phosphorylation
Y1132 Phosphorylation
S1133 Phosphorylation
S1134 Phosphorylation Q13315 (ATM)
S1141 Phosphorylation Q13315 (ATM)
S1147 Phosphorylation Q13315 (ATM)
S1149 Phosphorylation
S1155 Phosphorylation
S1156 Phosphorylation
T1159 Phosphorylation
K1160 Methylation
K1173 Acetylation
K1177 Acetylation
S1187 Phosphorylation
K1188 Acetylation
S1192 Phosphorylation
S1207 Phosphorylation P24941 (CDK2)
K1210 Acetylation
T1215 Phosphorylation P24941 (CDK2)
S1218 Phosphorylation
S1223 Phosphorylation
S1226 Phosphorylation
S1227 Phosphorylation
S1229 Phosphorylation
S1232 Phosphorylation
S1239 Phosphorylation
S1241 Phosphorylation
S1251 Phosphorylation
S1252 Phosphorylation
S1254 Phosphorylation
S1256 Phosphorylation
T1259 Phosphorylation
S1302 Phosphorylation
K1309 Acetylation
S1347 Phosphorylation
K1354 Acetylation
S1368 Phosphorylation
K1376 Acetylation
T1378 Phosphorylation
T1387 Phosphorylation
S1401 Phosphorylation
S1403 Phosphorylation
Y1431 Phosphorylation
S1433 Phosphorylation
S1437 Phosphorylation
S1439 Phosphorylation
T1440 Phosphorylation
S1449 Phosphorylation
S1451 Phosphorylation
S1453 Phosphorylation
Y1456 Phosphorylation
T1457 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1) , P78527 (PRKDC)
S1463 Phosphorylation
S1465 Phosphorylation
S1467 Phosphorylation
S1475 Phosphorylation
Y1476 Phosphorylation
S1479 Phosphorylation
S1481 Phosphorylation
S1482 Phosphorylation
K1502 Acetylation
K1504 Acetylation
K1529 Acetylation
S1532 Phosphorylation
S1534 Phosphorylation
S1536 Phosphorylation
S1548 Phosphorylation
T1550 Phosphorylation

研究背景

功能:

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells.

翻译修饰:

Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylation increases its interaction with PSIP1.

细胞定位:

Nucleus.
Note: A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed.

亚基结构:

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. This subunit specifically interacts with a number of nuclear receptors in a ligand-dependent fashion including AR, ESR1, ESR2, PPARA, PPARG, RORA, RXRA, RXRG, THRA, THRB and VDR. Interacts with CTNNB1, GABPA, GLI3, PPARGC1A and TP53. Interacts with YWHAH. Interacts with CLOCK; this interaction requires the presence of THRAP3 (By similarity). Interacts with GATA1 and CCAR1. Interacts with NR4A3 (By similarity). Interacts (via IBM motif) with PSIP1 (via IBD domain); phosphorylation increases its affinity for PSIP1.

蛋白家族:

Belongs to the Mediator complex subunit 1 family.

研究领域

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

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