FHL2 Antibody - #DF13015

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产品: FHL2 抗体
货号: DF13015
描述: Rabbit polyclonal antibody to FHL2
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 32 kDa; 32kD(Calculated).
蛋白号: Q14192
RRID: AB_2845976

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
FHL2 Antibody detects endogenous levels of total FHL2.
RRID:
AB_2845976
引用格式: Affinity Biosciences Cat# DF13015, RRID:AB_2845976.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AAG 11; AAG11; Aging associated gene 11; Down regulated in rhabdomyosarcoma LIM protein; Downregulated in rhabdomyosarcoma LIM protein; DRAL; FHL 2; FHL-2; Fhl2; FHL2 protein; FHL2_HUMAN; Four and a half LIM domain protein 2; Four and a half LIM domains 2; Four and a half LIM domains protein 2; KIAA0990; LIM domain protein DRAL; Skeletal muscle LIM protein 3; Skeletal muscle LIM-protein 3; SLIM 3; SLIM-3; SLIM3;

抗原和靶标

免疫原:
Uniprot:

Q14192(FHL2_HUMAN) >>Visit HPA database.

基因/基因ID:
FHL2(2274)
表达:
Q14192 FHL2_HUMAN:

Expressed in skeletal muscle and heart.

序列:
MTERFDCHHCNESLFGKKYILREESPYCVVCFETLFANTCEECGKPIGCDCKDLSYKDRHWHEACFHCSQCRNSLVDKPFAAKEDQLLCTDCYSNEYSSKCQECKKTIMPGTRKMEYKGSSWHETCFICHRCQQPIGTKSFIPKDNQNFCVPCYEKQHAMQCVQCKKPITTGGVTYREQPWHKECFVCTACRKQLSGQRFTARDDFAYCLNCFCDLYAKKCAGCTNPISGLGGTKYISFEERQWHNDCFNCKKCSLSLVGRGFLTERDDILCPDCGKDI

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
89
Sheep
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14192 作为底物

Site PTM Type Enzyme
S13 Phosphorylation
K17 Ubiquitination
Y27 Phosphorylation
Y56 Phosphorylation
K57 Acetylation
S74 Phosphorylation
K78 Sumoylation
K78 Ubiquitination
Y93 Phosphorylation
Y97 Phosphorylation
K100 Ubiquitination
K106 Ubiquitination
T107 Phosphorylation
T112 Phosphorylation
K118 Sumoylation
T138 Phosphorylation
K139 Sumoylation
K139 Ubiquitination
S140 Phosphorylation
K144 Sumoylation
K144 Ubiquitination
Y154 Phosphorylation
K156 Ubiquitination
K166 Ubiquitination
K167 Sumoylation
K167 Ubiquitination
T175 Phosphorylation
Y176 Phosphorylation
T189 Phosphorylation
K220 Sumoylation
K220 Ubiquitination
K235 Ubiquitination
Y236 Phosphorylation
S238 Phosphorylation
K252 Ubiquitination
K253 Sumoylation
K253 Ubiquitination
S255 Phosphorylation
K277 Acetylation
K277 Ubiquitination

研究背景

功能:

May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes.

细胞定位:

Cytoplasm. Nucleus. Cytoplasm>Myofibril>Sarcomere>Z line.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in skeletal muscle and heart.

亚基结构:

Interacts with ZNF638 and TTN/titin. Interacts with E4F1. Interacts with GRB7. Interacts with SIRT1 and FOXO1. Interacts with CEFIP. Interacts with calcineurin (By similarity). Interacts with FOXK1 (By similarity).

蛋白家族:

The third LIM zinc-binding mediates interaction with E4F1.

研究领域

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

文献引用

1). Cloning, Tissue Expression and Protein Localization of FHL2 Gene in Yaks. 畜牧兽医学报, 2023

Application: WB    Species: yak    Sample:

Fig. 1 Electrophoresis results of yak FHL2 PCR products

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