产品: 磷酸化 STAT4 (Tyr693) 抗体
货号: AF3441
描述: Rabbit polyclonal antibody to Phospho-STAT4 (Tyr693)
应用: WB IHC IF/ICC IP
反应: Human, Mouse, Rat
预测: Pig, Horse, Rabbit, Chicken
分子量: 86kDa; 86kD(Calculated).
蛋白号: Q14765
RRID: AB_2834883

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Horse(100%), Rabbit(100%), Chicken(89%)
克隆:
Polyclonal
特异性:
Phospho-STAT4 (Tyr693) Antibody detects endogenous levels of STAT4 only when phosphorylated at Tyrosine 693.
RRID:
AB_2834883
引用格式: Affinity Biosciences Cat# AF3441, RRID:AB_2834883.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Signal transducer and activator of transcription 4; SLEB11; STAT4; STAT4_HUMAN;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
描述:
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.
序列:
MSQWNQVQQLEIKFLEQVDQFYDDNFPMEIRHLLAQWIENQDWEAASNNETMATILLQNLLIQLDEQLGRVSKEKNLLLIHNLKRIRKVLQGKFHGNPMHVAVVISNCLREERRILAAANMPVQGPLEKSLQSSSVSERQRNVEHKVAAIKNSVQMTEQDTKYLEDLQDEFDYRYKTIQTMDQSDKNSAMVNQEVLTLQEMLNSLDFKRKEALSKMTQIIHETDLLMNTMLIEELQDWKRRQQIACIGGPLHNGLDQLQNCFTLLAESLFQLRRQLEKLEEQSTKMTYEGDPIPMQRTHMLERVTFLIYNLFKNSFVVERQPCMPTHPQRPLVLKTLIQFTVKLRLLIKLPELNYQVKVKASIDKNVSTLSNRRFVLCGTNVKAMSIEESSNGSLSVEFRHLQPKEMKSSAGGKGNEGCHMVTEELHSITFETQICLYGLTIDLETSSLPVVMISNVSQLPNAWASIIWYNVSTNDSQNLVFFNNPPPATLSQLLEVMSWQFSSYVGRGLNSDQLHMLAEKLTVQSSYSDGHLTWAKFCKEHLPGKSFTFWTWLEAILDLIKKHILPLWIDGYVMGFVSKEKERLLLKDKMPGTFLLRFSESHLGGITFTWVDHSESGEVRFHSVEPYNKGRLSALPFADILRDYKVIMAENIPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIPISTIRSDSTEPHSPSDLLPMSPSVYAVLRENLSPTTIETAMKSPYSAE

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Rabbit
100
Chicken
89
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14765 作为底物

Site PTM Type Enzyme
S130 Phosphorylation
S137 Phosphorylation
K151 Ubiquitination
T284 Phosphorylation
T287 Phosphorylation
Y288 Phosphorylation
T298 Phosphorylation
S362 Phosphorylation
T369 Phosphorylation
S371 Phosphorylation
S386 Phosphorylation
S394 Phosphorylation
S396 Phosphorylation
K562 Ubiquitination
Y645 Phosphorylation
Y693 Phosphorylation O60674 (JAK2)
S713 Phosphorylation
S721 Phosphorylation Q16539 (MAPK14) , P52564 (MAP2K6)
S733 Phosphorylation
S743 Phosphorylation
Y745 Phosphorylation
S746 Phosphorylation

研究背景

功能:

Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling.

翻译修饰:

Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity (By similarity).

细胞定位:

Cytoplasm. Nucleus.
Note: Translocated into the nucleus in response to phosphorylation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Forms a homodimer or a heterodimer with a related family member. Interacts with ARL2BP (By similarity). The SH2 domain interacts, in vitro, with IL12RB2 via a short cytoplasmic domain.

蛋白家族:

Belongs to the transcription factor STAT family.

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th1 and Th2 cell differentiation.   (View pathway)

文献引用

1). Bone marrow stem cells secretome accelerates simulated birth trauma-induced stress urinary incontinence recovery in rats. Aging-US (PubMed: 33793419) [IF=5.2]

Application: WB    Species: Rat    Sample: fibroblasts

Figure 4 BMSC-CM activated fibroblasts by activating the JAK2/STAT4 pathway. (A) The expression of JAK2, p-JAK2, STAT4, and p-STAT4 in fibroblasts treated with different media was determined by western blotting. (B) The expression of three most significant DEGs (POSTN, COMP, and TGFBI) in fibroblasts treated with JAK2 inhibitor AG490 (10 μM). (C) Wound–scratch assay in fibroblasts treated with or without JAK2 inhibitor AG490 (10 μM) at 0 h, 24 h, and 48 h (magnification, ×40). Scale bar = 400 μm. (D) The percentage of migration area in different groups. (E) The CCK-8 assay showing reduced proliferation of fibroblasts treated with BMSC-CM after treatment with AG490. (F) Collagen synthesis in fibroblasts after AG490 treatment was determined by RT-PCR. Data are shown as means ± standard deviation (SD). *P < 0.05; **P < 0.01; ***P < 0.001. BMSC-CM, bone marrow stem cell-conditioned medium; con, control medium treatment group; CM, BMSC-CM treatment group.

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