DYNLL2 Antibody - #DF12966

Figure 7 Immunostaining and immunoblot of ribosomal and motility-related proteins in SPZED and SPZEJ (A–F) The upper panels show representative immunostaining of large ribosomal subunit protein eL31 (Rpl31, A), small ribosomal subunit protein uS5 (Rps2, B), 40S ribosomal protein S13 (Rps13, C), sperm-associated antigen 6 (Spag6, D), dynein light chain 2 (Dynll2, E) and α-tubulin (Tuba, F) in SPZED and SPZEJ. The brightfield (left) and epifluorescence (right) images with the nucleus counterstained with DAPI (blue) are shown. Scale bars, 5 μm. The lower panels depict the corresponding immunoblots (left) and quantitation normalized to prohibitin (Phb) or histone H3 (H3) (right). The data points (red dots) are presented as box and whisker plots/scatter dots with horizontal line (inside box) indicating median and outliers (n = 3–4 fish different from those employed for MS analyses). Statistical differences in protein abundance between SPZED and SPZEJ were determined by an unpaired Student’s t test (p-values indicated above each plot). Molecular mass markers (kDa) are on the left, and the corresponding MS quantification data are presented below the plots. (G and H) Representative immunostaining of small mitochondrial ribosomal subunit uS5m (mRps5, A) and large mitochondrial ribosomal subunit bL28m (mRpl28, B) in SPZED and SPZEJ. The nucleus was counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; blue), whereas the spermatozoon mitochondrion was labeled with MitoTracker Green FM (green). Scale bars, 5 μm. To the right of each panel the corresponding immunoblots using PhB as loading control are shown. Molecular mass markers (kDa) are on the left.