DIO2 Antibody - #DF12941

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产品: DIO2 抗体
货号: DF12941
描述: Rabbit polyclonal antibody to DIO2
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Rabbit, Dog, Chicken
分子量: 31 kDa; 31kD(Calculated).
蛋白号: Q92813
RRID: AB_2845902

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实验方案
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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Rabbit(%), Dog(%), Chicken(%)
克隆:
Polyclonal
特异性:
DIO2 Antibody detects endogenous levels of total DIO2.
RRID:
AB_2845902
引用格式: Affinity Biosciences Cat# DF12941, RRID:AB_2845902.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

5DII; D2; Deiodinase, iodothyronine, type II; DIO2; DIOII; EC 1.97.1.10; IOD2_HUMAN; ITDI2; SelY; Thyroxine deiodinase, type II; TXDI2; Type 2 DI; Type 2 iodothyronine deiodinase; Type II 5' deiodinase; Type II iodothyronine deiodinase; Type-II 5''-deiodinase;

抗原和靶标

免疫原:

A synthesized peptide derived from human DIO2, corresponding to a region within N-terminal amino acids.

Uniprot:

Q92813(IOD2_HUMAN) >>Visit HPA database.

基因/基因ID:
DIO2(1734)
表达:
Q92813 IOD2_HUMAN:

Isoform 1 is expressed in the lung, trachea, kidney, heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain (PubMed:8755651, PubMed:11165050). Isoform 2 is expressed in the brain, heart, kidney and trachea (PubMed:11165050).

序列:
MGILSVDLLITLQILPVFFSNCLFLALYDSVILLKHVVLLLSRSKSTRGEWRRMLTSEGLRCVWKSFLLDAYKQVKLGEDAPNSSVVHVSSTEGGDNSGNGTQEKIAEGATCHLLDFASPERPLVVNFGSATUPPFTSQLPAFRKLVEEFSSVADFLLVYIDEAHPSDGWAIPGDSSLSFEVKKHQNQEDRCAAAQQLLERFSLPPQCRVVADRMDNNANIAYGVAFERVCIVQRQKIAYLGGKGPFSYNLQEVRHWLEKNFSKRUKKTRLAG

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Bovine
90
Chicken
90
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). Essential for providing the brain with appropriate levels of T3 during the critical period of development.

翻译修饰:

Ubiquitinated by MARCHF6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33.

细胞定位:

Membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform 1 is expressed in the lung, trachea, kidney, heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain. Isoform 2 is expressed in the brain, heart, kidney and trachea.

亚基结构:

Interacts with USP20 and USP33. Interacts with MARCHF6.

蛋白家族:

Belongs to the iodothyronine deiodinase family.

研究领域

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

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