产品: | 磷酸化 Cortactin (Tyr421) 抗体 |
货号: | AF3436 |
描述: | Rabbit polyclonal antibody to Phospho-Cortactin (Tyr421) |
应用: | WB IF/ICC |
反应: | Human, Mouse, Rat |
预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
分子量: | 85kDa; 62kD(Calculated). |
蛋白号: | Q14247 |
RRID: | AB_2834878 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3436, RRID:AB_2834878.
展开/折叠
Amplaxin; CTTN; EMS 1; EMS1; FLJ34459; Mammary tumor and squamous cell carcinoma associated; Oncogene EMS1; p80/85 src substrate; Src substrate cortactin; SRC8_HUMAN;
抗原和靶标
- Q14247 SRC8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MWKASAGHAVSIAQDDAGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAVGHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQRDYSKGFGGKYGIDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYKTGFGGKFGVQSERQDSAAVGFDYKEKLAKHESQQDYSKGFGGKYGVQKDRMDKNASTFEDVTQVSSAYQKTVPVEAVTSKTSNIRANFENLAKEKEQEDRRKAEAERAQRMAKERQEQEEARRKLEEQARAKTQTPPVSPAPQPTEERLPSSPVYEDAASFKAELSYRGPVSGTEPEPVYSMEAADYREASSQQGLAYATEAVYESAEAPGHYPAEDSTYDEYENDLGITAVALYDYQAAGDDEISFDPDDIITNIEMIDDGWWRGVCKGRYGLFPANYVELRQ
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q14247 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K3 | Methylation | Uniprot | |
S11 | Phosphorylation | Uniprot | |
T24 | Phosphorylation | Uniprot | |
S33 | Phosphorylation | Uniprot | |
K35 | Ubiquitination | Uniprot | |
K42 | Ubiquitination | Uniprot | |
T43 | Phosphorylation | Uniprot | |
S47 | Phosphorylation | Uniprot | |
K57 | Ubiquitination | Uniprot | |
T68 | Phosphorylation | Uniprot | |
K70 | Methylation | Uniprot | |
K72 | Methylation | Uniprot | |
K79 | Methylation | Uniprot | |
K79 | Ubiquitination | Uniprot | |
S81 | Phosphorylation | Uniprot | |
Y84 | Phosphorylation | Uniprot | |
K87 | Acetylation | Uniprot | |
K87 | Methylation | Uniprot | |
K87 | Ubiquitination | Uniprot | |
S98 | Phosphorylation | Uniprot | |
Y104 | Phosphorylation | Uniprot | |
S106 | Phosphorylation | Uniprot | |
K107 | Methylation | Uniprot | |
K107 | Ubiquitination | Uniprot | |
S109 | Phosphorylation | Uniprot | |
K110 | Ubiquitination | Uniprot | |
C112 | S-Nitrosylation | Uniprot | |
S113 | Phosphorylation | Uniprot | |
S117 | Phosphorylation | Uniprot | |
R119 | Methylation | Uniprot | |
K124 | Acetylation | Uniprot | |
K124 | Methylation | Uniprot | |
K124 | Ubiquitination | Uniprot | |
R131 | Methylation | Uniprot | |
S135 | Phosphorylation | Uniprot | |
Y141 | Phosphorylation | Uniprot | |
K144 | Acetylation | Uniprot | |
K144 | Sumoylation | Uniprot | |
K144 | Ubiquitination | Uniprot | |
S150 | Phosphorylation | Uniprot | |
K152 | Ubiquitination | Uniprot | |
Y154 | Phosphorylation | Uniprot | |
S155 | Phosphorylation | Uniprot | |
S156 | Phosphorylation | Uniprot | |
K161 | Acetylation | Uniprot | |
K161 | Methylation | Uniprot | |
K161 | Ubiquitination | Uniprot | |
Y162 | Phosphorylation | Uniprot | |
R168 | Methylation | Uniprot | |
K171 | Acetylation | Uniprot | |
K171 | Ubiquitination | Uniprot | |
S172 | Phosphorylation | Uniprot | |
Y178 | Phosphorylation | Uniprot | |
K181 | Acetylation | Uniprot | |
K181 | Sumoylation | Uniprot | |
T182 | Phosphorylation | Uniprot | |
S187 | Phosphorylation | Uniprot | |
Y191 | Phosphorylation | Uniprot | |
S192 | Phosphorylation | Uniprot | |
K193 | Ubiquitination | Uniprot | |
K198 | Acetylation | Uniprot | |
K198 | Ubiquitination | Uniprot | |
Y199 | Phosphorylation | Uniprot | |
K203 | Acetylation | Uniprot | |
K208 | Ubiquitination | Uniprot | |
S209 | Phosphorylation | Uniprot | |
Y215 | Phosphorylation | Uniprot | |
K218 | Acetylation | Uniprot | |
K218 | Sumoylation | Uniprot | |
T219 | Phosphorylation | Uniprot | |
S224 | Phosphorylation | Uniprot | |
K226 | Ubiquitination | Uniprot | |
Y228 | Phosphorylation | Uniprot | |
K230 | Ubiquitination | Uniprot | |
K235 | Acetylation | Uniprot | |
K235 | Methylation | Uniprot | |
K235 | Ubiquitination | Uniprot | |
K245 | Ubiquitination | Uniprot | |
S261 | Phosphorylation | P41743 (PRKCI) | Uniprot |
K263 | Ubiquitination | Uniprot | |
Y265 | Phosphorylation | Uniprot | |
K266 | Ubiquitination | Uniprot | |
T267 | Phosphorylation | Uniprot | |
K272 | Acetylation | Uniprot | |
K272 | Methylation | Uniprot | |
K272 | Ubiquitination | Uniprot | |
S277 | Phosphorylation | Uniprot | |
S282 | Phosphorylation | Uniprot | |
Y289 | Phosphorylation | Uniprot | |
K290 | Acetylation | Uniprot | |
K290 | Ubiquitination | Uniprot | |
K295 | Sumoylation | Uniprot | |
S298 | Phosphorylation | O14757 (CHEK1) , Q15139 (PRKD1) | Uniprot |
Y302 | Phosphorylation | Uniprot | |
S303 | Phosphorylation | Uniprot | |
K304 | Acetylation | Uniprot | |
K304 | Ubiquitination | Uniprot | |
K309 | Acetylation | Uniprot | |
K309 | Methylation | Uniprot | |
Y310 | Phosphorylation | Uniprot | |
K319 | Acetylation | Uniprot | |
S322 | Phosphorylation | Uniprot | |
T323 | Phosphorylation | Uniprot | |
S331 | Phosphorylation | Uniprot | |
S332 | Phosphorylation | Uniprot | |
Y334 | Phosphorylation | P12931 (SRC) | Uniprot |
K336 | Ubiquitination | Uniprot | |
T337 | Phosphorylation | Uniprot | |
S345 | Phosphorylation | Uniprot | |
K346 | Ubiquitination | Uniprot | |
S348 | Phosphorylation | Q15139 (PRKD1) | Uniprot |
T399 | Phosphorylation | Uniprot | |
T401 | Phosphorylation | Uniprot | |
S405 | Phosphorylation | Q05655 (PRKCD) , P06493 (CDK1) , P28482 (MAPK1) , Q13153 (PAK1) , P24941 (CDK2) , P27361 (MAPK3) | Uniprot |
T411 | Phosphorylation | Uniprot | |
R414 | Methylation | Uniprot | |
S417 | Phosphorylation | Uniprot | |
S418 | Phosphorylation | P27361 (MAPK3) , P28482 (MAPK1) , Q13153 (PAK1) | Uniprot |
Y421 | Phosphorylation | P00519 (ABL1) , P42684 (ABL2) | Uniprot |
S426 | Phosphorylation | Uniprot | |
S432 | Phosphorylation | Uniprot | |
Y433 | Phosphorylation | Uniprot | |
S438 | Phosphorylation | Uniprot | |
T440 | Phosphorylation | Uniprot | |
Y446 | Phosphorylation | P12931 (SRC) , P11362 (FGFR1) | Uniprot |
S447 | Phosphorylation | Uniprot | |
Y453 | Phosphorylation | Uniprot | |
Y464 | Phosphorylation | Uniprot | |
Y470 | Phosphorylation | P42684 (ABL2) , P12931 (SRC) , P00519 (ABL1) | Uniprot |
Y479 | Phosphorylation | Uniprot | |
T485 | Phosphorylation | Uniprot | |
Y486 | Phosphorylation | P42684 (ABL2) , P12931 (SRC) , P00519 (ABL1) | Uniprot |
Y538 | Phosphorylation | Uniprot | |
Y545 | Phosphorylation | Uniprot | |
R549 | Methylation | Uniprot |
研究背景
Contributes to the organization of the actin cytoskeleton and cell shape. Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones (By similarity). Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density (By similarity). Plays a role in the invasiveness of cancer cells, and the formation of metastases. Plays a role in focal adhesion assembly and turnover (By similarity). In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement. Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane. Plays a role in receptor-mediated endocytosis via clathrin-coated pits (By similarity). Required for stabilization of KCNH1 channels at the cell membrane.
Phosphorylated by PKN2 at both serine and threonine residues in a GTP-bound Rac1-dependent manner in hyaluronan-induced astrocytes and hence down-regulated CTTN ability to associates with filamentous actin (By similarity). Phosphorylated on tyrosine residues in response to CHRM1 activation (By similarity). Phosphorylated by PTK2/FAK1 in response to cell adhesion (By similarity). Phosphorylated by FER. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation. Phosphorylated in response to FGR activation. Phosphorylation by SRC promotes MYLK binding.
Cytoplasm>Cytoskeleton. Cell projection>Lamellipodium. Cell projection>Ruffle. Cell projection>Dendrite. Cell projection. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell projection>Podosome. Cell junction. Cell junction>Focal adhesion. Membrane>Clathrin-coated pit. Cell projection>Dendritic spine. Cytoplasm>Cell cortex.
Note: Colocalizes transiently with PTK2/FAK1 at focal adhesions (By similarity). Associated with membrane ruffles and lamellipodia. In the presence of CTTNBP2NL, colocalizes with stress fibers (By similarity). In the presence of CTTNBP2, localizes at the cell cortex (By similarity). In response to neuronal activation by glutamate, redistributes from dendritic spines to the dendritic shaft (By similarity). Colocalizes with DNM2 at the basis of filopodia in hippocampus neuron growth zones (By similarity).
Interacts with SHANK2 and SHANK3 (via its SH3 domain). Also interacts with FGD1. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Interacts with ABL2 (By similarity). Interacts with KCNA2 (via non-phosphorylated C-terminus). Interacts with PLXDC2 and SRCIN1. Interacts with SAMSN1 (via SH3 domain). Interacts (via SH3 domain) with ASAP1 (via Pro-rich region). Interacts (via SH3 domain) with DNM2 (By similarity). Interacts with ACTN1 (By similarity). Interacts with FER. Forms a complex made of ABL1 and MYLK. Interacts with CTTNBP2NL; this interaction may target CTTN to stress fibers. Interacts with CTTNBP2; this interaction may target CTTN at the cell cortex or dendritic spines. Interacts with KCNH1.
The SH3 motif may mediate binding to the cytoskeleton.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
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