Phospho-Pin1 (Ser16) Antibody - #AF3430
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3430, RRID:AB_2834872.
展开/折叠
DOD; DODO, Drosophila, homolog of; FLJ40239; FLJ77628; MGC10717; NIMA interacting 1; Peptidyl prolyl cis trans isomerase NIMA interacting 1; Peptidyl prolyl cis/trans isomerase NIMA interacting; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase pin1; Peptidylprolyl cis/trans isomerase NIMA interacting 1; Pin 1; Pin1; PIN1_HUMAN; PPIase Pin1; Prolyl isomerase; Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1; Protein NIMA interacting 1; Rotamase Pin1; UBL 5; UBL5;
抗原和靶标
The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells.
- Q13526 PIN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRRPSSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFALRTGEMSGPVFTDSGIHIILRTE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13526 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Sumoylation | Uniprot | |
| K6 | Ubiquitination | Uniprot | |
| K13 | Ubiquitination | Uniprot | |
| S16 | Phosphorylation | P41279 (MAP3K8) , O14965 (AURKA) , P17612 (PRKACA) | Uniprot |
| Y23 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| K46 | Acetylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| K63 | Acetylation | Uniprot | |
| K63 | Sumoylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| S65 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S71 | Phosphorylation | P53355 (DAPK1) | Uniprot |
| S72 | Phosphorylation | Uniprot | |
| K82 | Acetylation | Uniprot | |
| K82 | Sumoylation | Uniprot | |
| K82 | Ubiquitination | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| S114 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| S138 | Phosphorylation | Q16584 (MAP3K11) | Uniprot |
| R142 | Methylation | Uniprot |
研究背景
Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs. By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes Ref. 21). Displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation. Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner. Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN. May facilitate the ubiquitination and proteasomal degradation of RBBP8/CtIP through CUL3/KLHL15 E3 ubiquitin-protein ligase complex, hence favors DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR).
Phosphorylation at Ser-71 by DAPK1 results in inhibition of its catalytic activity, nuclear localization, and its ability to induce centrosome amplification, chromosome instability and cell transformation.
Nucleus. Nucleus speckle. Cytoplasm.
Note: Colocalizes with NEK6 in the nucleus (PubMed:16476580). Mainly localized in the nucleus but phosphorylation at Ser-71 by DAPK1 results in inhibition of its nuclear localization (PubMed:21497122).
The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells.
Interacts with STIL. Interacts with KIF20B. Interacts with NEK6. Interacts (via WW domain) with PRKX. Interacts with BTK. Interacts (via PpiC domain) with DAPK1. Interacts with the phosphorylated form of RAF1. Interacts (via WW domain) with ATCAY; upon NGF stimulation. Interacts with PML (isoform PML-4) and BCL-6. Interacts with FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation. Directly interacts with RBBP8/CtIP; this interaction depends upon RBBP8 phosphorylation.
The WW domain is required for the interaction with STIL and KIF20B.
研究领域
· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway. (View pathway)
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