Phospho-BIK (Thr33) Antibody - #AF3428

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产品: 磷酸化 BIK (Thr33) 抗体
货号: AF3428
描述: Rabbit polyclonal antibody to Phospho-BIK (Thr33)
应用: WB IHC IF/ICC
反应: Human
分子量: 30kDa; 18kD(Calculated).
蛋白号: Q13323
RRID: AB_2834870

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MSDS
说明书
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
克隆:
Polyclonal
特异性:
Phospho-BIK (Thr33) Antibody detects endogenous levels of BIK only when phosphorylated at Threonine 33.
RRID:
AB_2834870
引用格式: Affinity Biosciences Cat# AF3428, RRID:AB_2834870.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Apoptosis inducer NBK; BBC1; Bcl-2-interacting killer; BCL2 interacting killer; bhikhari; BIK; Bik-like killer protein; BIK_HUMAN; BIP 1; BiP1; BP 4; BP4; cb 60; NBK;

抗原和靶标

免疫原:
Uniprot:

Q13323(BIK_HUMAN) >>Visit HPA database.

基因/基因ID:
BIK(638)
描述:
The protein encoded by this gene is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein-Barr virus in order to enhance programed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins.
序列:
MSEVRPLSRDILMETLLYEQLLEPPTMEVLGMTDSEEDLDPMEDFDSLECMEGSDALALRLACIGDEMDVSLRAPRLAQLSEVAMHSLGLAFIYDQTEDIRDVLRSFMDGFTTLKENIMRFWRSPNPGSWVSCEQVLLALLLLLALLLPLLSGGLHLLLK

翻译修饰 - Q13323 作为底物

Site PTM Type Enzyme
S2 Phosphorylation
T33 Phosphorylation P68400 (CSNK2A1)
S35 Phosphorylation P68400 (CSNK2A1)
K115 Ubiquitination
S124 Phosphorylation

研究背景

功能:

Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.

翻译修饰:

Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage is a necessary step prior its degradation by the proteosome-dependent mechanism.

细胞定位:

Endomembrane system>Single-pass membrane protein. Mitochondrion membrane>Single-pass membrane protein.
Note: Around the nuclear envelope, and in cytoplasmic membranes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with RHBDL4/RHBDD1.

蛋白家族:

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

研究领域

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

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