产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3424, RRID:AB_2834866.
展开/折叠
ADRB2; Alpha PAK; Alpha-PAK; MGC130000; MGC130001; p21 activated kinase 1; p21 protein (Cdc42/Rac) activated kinase 1; p21-activated kinase 1; p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related); p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p65 PAK; p65-PAK; P68-PAK; PAK alpha; PAK-1; Pak1; PAK1_HUMAN; Paka; PAKalpha; Protein kinase MUK2; Rac/p21-activated kinase; Serine/threonine-protein kinase PAK 1; STE20 homolog yeast;
抗原和靶标
Overexpressed in gastric cancer cells and tissues (at protein level) (PubMed:25766321).
- Q13153 PAK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSNNGLDIQDKPPAPPMRNTSTMIGAGSKDAGTLNHGSKPLPPNPEEKKKKDRFYRSILPGDKTNKKKEKERPEISLPSDFEHTIHVGFDAVTGEFTGMPEQWARLLQTSNITKSEQKKNPQAVLDVLEFYNSKKTSNSQKYMSFTDKSAEDYNSSNALNVKAVSETPAVPPVSEDEDDDDDDATPPPVIAPRPEHTKSVYTRSVIEPLPVTPTRDVATSPISPTENNTTPPDALTRNTEKQKKKPKMSDEEILEKLRSIVSVGDPKKKYTRFEKIGQGASGTVYTAMDVATGQEVAIKQMNLQQQPKKELIINEILVMRENKNPNIVNYLDSYLVGDELWVVMEYLAGGSLTDVVTETCMDEGQIAAVCRECLQALEFLHSNQVIHRDIKSDNILLGMDGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMIEGEPPYLNENPLRALYLIATNGTPELQNPEKLSAIFRDFLNRCLEMDVEKRGSAKELLQHQFLKIAKPLSSLTPLIAAAKEATKNNH
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13153 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Acetylation | Uniprot | |
S2 | Phosphorylation | Uniprot | |
K11 | Ubiquitination | Uniprot | |
T20 | Phosphorylation | Uniprot | |
S21 | Phosphorylation | Q13153 (PAK1) , Q13976-2 (PRKG1) , P31749 (AKT1) | Uniprot |
S28 | Phosphorylation | Uniprot | |
K29 | Acetylation | Uniprot | |
K29 | Ubiquitination | Uniprot | |
S38 | Phosphorylation | Uniprot | |
K39 | Ubiquitination | Uniprot | |
S57 | Phosphorylation | Q13153 (PAK1) | Uniprot |
K63 | Acetylation | Uniprot | |
S79 | Phosphorylation | Uniprot | |
T84 | Phosphorylation | O95747 (OXSR1) | Uniprot |
T109 | Phosphorylation | Q15831 (STK11) | Uniprot |
S110 | Phosphorylation | Uniprot | |
S115 | Phosphorylation | Uniprot | |
Y131 | Phosphorylation | Uniprot | |
S133 | Phosphorylation | Uniprot | |
T136 | Phosphorylation | Uniprot | |
S137 | Phosphorylation | Uniprot | |
S139 | Phosphorylation | Uniprot | |
Y142 | Phosphorylation | Uniprot | |
S144 | Phosphorylation | Q13153 (PAK1) | Uniprot |
T146 | Phosphorylation | Uniprot | |
K148 | Ubiquitination | Uniprot | |
S149 | Phosphorylation | Q13153 (PAK1) | Uniprot |
Y153 | Phosphorylation | O60674 (JAK2) | Uniprot |
S155 | Phosphorylation | Uniprot | |
S156 | Phosphorylation | Uniprot | |
S174 | Phosphorylation | Q9BWU1 (CDK19) | Uniprot |
T185 | Phosphorylation | Uniprot | |
T197 | Phosphorylation | Uniprot | |
S199 | Phosphorylation | Q13153 (PAK1) | Uniprot |
Y201 | Phosphorylation | O60674 (JAK2) | Uniprot |
S204 | Phosphorylation | Q13153 (PAK1) | Uniprot |
T212 | Phosphorylation | P28482 (MAPK1) | Uniprot |
T214 | Phosphorylation | Uniprot | |
T219 | Phosphorylation | Uniprot | |
S220 | Phosphorylation | Uniprot | |
S223 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
T225 | Phosphorylation | Uniprot | |
T229 | Phosphorylation | Uniprot | |
T230 | Phosphorylation | Uniprot | |
T236 | Phosphorylation | Uniprot | |
S249 | Phosphorylation | Uniprot | |
K256 | Acetylation | Uniprot | |
K256 | Sumoylation | Uniprot | |
K256 | Ubiquitination | Uniprot | |
S259 | Phosphorylation | Uniprot | |
S262 | Phosphorylation | Uniprot | |
Y285 | Phosphorylation | O60674 (JAK2) | Uniprot |
T292 | Phosphorylation | P27361 (MAPK3) | Uniprot |
K309 | Ubiquitination | Uniprot | |
K391 | Ubiquitination | Uniprot | |
T415 | Phosphorylation | Uniprot | |
K420 | Acetylation | Uniprot | |
K420 | Ubiquitination | Uniprot | |
S422 | Phosphorylation | Uniprot | |
T423 | Phosphorylation | O15530 (PDPK1) , Q8IWQ3 (BRSK2) , Q13153 (PAK1) | Uniprot |
T427 | Phosphorylation | Uniprot | |
Y474 | Phosphorylation | Uniprot | |
S491 | Phosphorylation | Uniprot | |
K508 | Ubiquitination | Uniprot | |
S511 | Phosphorylation | Uniprot | |
K513 | Acetylation | Uniprot | |
S528 | Phosphorylation | Uniprot | |
S529 | Phosphorylation | Uniprot | |
T531 | Phosphorylation | Uniprot |
翻译修饰 - Q13153 作为激酶
Substrate | Site | Source |
---|---|---|
O14965 (AURKA) | T288 | Uniprot |
O14965 (AURKA) | S342 | Uniprot |
O15143 (ARPC1B) | T21 | Uniprot |
O95863 (SNAI1) | S246 | Uniprot |
P03372-1 (ESR1) | S305 | Uniprot |
P04049 (RAF1) | S338 | Uniprot |
P04049 (RAF1) | S339 | Uniprot |
P08670 (VIM) | S26 | Uniprot |
P08670 (VIM) | S39 | Uniprot |
P08670 (VIM) | S51 | Uniprot |
P08670 (VIM) | S56 | Uniprot |
P08670 (VIM) | S66 | Uniprot |
P08670 (VIM) | S73 | Uniprot |
P10398 (ARAF) | S299 | Uniprot |
P15056 (BRAF) | S446 | Uniprot |
P16949 (STMN1) | S16 | Uniprot |
P16949 (STMN1) | S38 | Uniprot |
P17600-2 (SYN1) | S605 | Uniprot |
P18669 (PGAM1) | S23 | Uniprot |
P18669 (PGAM1) | S118 | Uniprot |
P19086 (GNAZ) | S16 | Uniprot |
P21333 (FLNA) | S2152 | Uniprot |
P21333 (FLNA) | S2292 | Uniprot |
P21333 (FLNA) | S2370 | Uniprot |
P24844 (MYL9) | S20 | Uniprot |
P29466-1 (CASP1) | S376 | Uniprot |
P31749 (AKT1) | S473 | Uniprot |
P35222 (CTNNB1) | S675 | Uniprot |
P35240-4 (NF2) | S435 | Uniprot |
P35240-6 (NF2) | S476 | Uniprot |
P35240-5 (NF2) | S477 | Uniprot |
P35240-3 (NF2) | S518 | Uniprot |
P36871 (PGM1) | T467 | Uniprot |
P42229 (STAT5A) | S780 | Uniprot |
P46108 (CRK) | S41 | Uniprot |
P49023 (PXN) | S258 | Uniprot |
P49023-2 (PXN) | S272 | Uniprot |
P51114 (FXR1) | S420 | Uniprot |
P52565 (ARHGDIA) | S101 | Uniprot |
P52565 (ARHGDIA) | S174 | Uniprot |
P53350 (PLK1) | S49 | Uniprot |
P53667 (LIMK1) | T508 | Uniprot |
P58012 (FOXL2) | S263 | Uniprot |
P63167 (DYNLL1) | S88 | Uniprot |
P68431 (HIST1H3J) | S11 | Uniprot |
P78545 (ELF3) | S207 | Uniprot |
Q02750-1 (MAP2K1) | S298 | Uniprot |
Q12778 (FOXO1) | S256 | Uniprot |
Q13153-1 (PAK1) | S21 | Uniprot |
Q13153-1 (PAK1) | S57 | Uniprot |
Q13153 (PAK1) | S144 | Uniprot |
Q13153 (PAK1) | S149 | Uniprot |
Q13153-1 (PAK1) | S165 | Uniprot |
Q13153 (PAK1) | S199 | Uniprot |
Q13153-1 (PAK1) | S204 | Uniprot |
Q13153 (PAK1) | T423 | Uniprot |
Q13233 (MAP3K1) | S67 | Uniprot |
Q13352 (ITGB3BP) | S28 | Uniprot |
Q13363 (CTBP1) | S158 | Uniprot |
Q13418 (ILK) | T173 | Uniprot |
Q13418 (ILK) | S246 | Uniprot |
Q14155 (ARHGEF7) | S518 | Uniprot |
Q14247 (CTTN) | S405 | Uniprot |
Q14247 (CTTN) | S418 | Uniprot |
Q15365 (PCBP1) | T60 | Uniprot |
Q15365 (PCBP1) | T127 | Uniprot |
Q15746-1 (MYLK) | S18 | Uniprot |
Q15746 (MYLK) | S1772 | Uniprot |
Q70Z35 (PREX2) | S1107 | Uniprot |
Q7Z628 (NET1) | S152 | Uniprot |
Q8IYU2 (HACE1) | S385 | Uniprot |
Q92934 (BAD) | S74 | Uniprot |
Q92934 (BAD) | S75 | Uniprot |
Q92934 (BAD) | S99 | Uniprot |
Q92934 (BAD) | S118 | Uniprot |
Q92934 (BAD) | S134 | Uniprot |
Q92974-3 (ARHGEF2) | S858 | Uniprot |
Q92974 (ARHGEF2) | S886 | Uniprot |
Q96A00 (PPP1R14A) | T38 | Uniprot |
Q96T58 (SPEN) | S3486 | Uniprot |
Q96T58 (SPEN) | T3568 | Uniprot |
Q99426 (TBCB) | S65 | Uniprot |
Q99426 (TBCB) | S128 | Uniprot |
Q99661 (KIF2C) | S111 | Uniprot |
Q99661 (KIF2C) | S192 | Uniprot |
Q9UQ80 (PA2G4) | T261 | Uniprot |
Q9Y2X7 (GIT1) | S508 | Uniprot |
Q9Y2X7 (GIT1) | S700 | Uniprot |
Q9Y6X9 (MORC2) | S739 | Uniprot |
研究背景
Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases. Involved in the reorganization of the actin cytoskeleton, actin stress fibers and of focal adhesion complexes. Phosphorylates the tubulin chaperone TBCB and thereby plays a role in the regulation of microtubule biogenesis and organization of the tubulin cytoskeleton. Plays a role in the regulation of insulin secretion in response to elevated glucose levels. Part of a ternary complex that contains PAK1, DVL1 and MUSK that is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. Phosphorylates MYL9/MLC2. Phosphorylates RAF1 at 'Ser-338' and 'Ser-339' resulting in: activation of RAF1, stimulation of RAF1 translocation to mitochondria, phosphorylation of BAD by RAF1, and RAF1 binding to BCL2. Phosphorylates SNAI1 at 'Ser-246' promoting its transcriptional repressor activity by increasing its accumulation in the nucleus. In podocytes, promotes NR3C2 nuclear localization. Required for atypical chemokine receptor ACKR2-induced phosphorylation of LIMK1 and cofilin (CFL1) and for the up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3, maybe through CFL1 phosphorylation and inactivation. Plays a role in RUFY3-mediated facilitating gastric cancer cells migration and invasion. In response to DNA damage, phosphorylates MORC2 which activates its ATPase activity and facilitates chromatin remodeling.
Autophosphorylated in trans, meaning that in a dimer, one kinase molecule phosphorylates the other one. Activated by autophosphorylation at Thr-423 in response to a conformation change, triggered by interaction with GTP-bound CDC42 or RAC1. Activated by phosphorylation at Thr-423 by BRSK2 and by PDPK1. Phosphorylated by JAK2 in response to PRL; this increases PAK1 kinase activity. Phosphorylated at Ser-21 by PKB/AKT; this reduces interaction with NCK1 and association with focal adhesion sites. Upon DNA damage, phosphorylated at Thr-212 and translocates to the nucleoplasm. Phosphorylated at tyrosine residues, which can be enhanced by NTN1 (By similarity).
Cytoplasm. Cell junction>Focal adhesion. Cell membrane. Cell projection>Ruffle membrane. Cell projection>Invadopodium. Nucleus>Nucleoplasm. Chromosome.
Note: Colocalizes with RUFY3, F-actin and other core migration components in invadopodia at the cell periphery (PubMed:25766321). Recruited to the cell membrane by interaction with CDC42 and RAC1. Recruited to focal adhesions upon activation. Colocalized with CIB1 within membrane ruffles during cell spreading upon readhesion to fibronectin. Upon DNA damage, translocates to the nucleoplasm when phosphorylated at Thr-212 where is co-recruited with MORC2 on damaged chromatin (PubMed:23260667).
Overexpressed in gastric cancer cells and tissues (at protein level).
Homodimer; homodimerization results in autoinhibition. Active as monomer. Component of cytoplasmic complexes, which also contains PXN, ARHGEF6 and GIT1. Interacts with NISCH (By similarity). Interacts with DVL1; mediates the formation of a DVL1, MUSK and PAK1 ternary complex involved in AChR clustering (By similarity). Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. Interacts with ARHGEF7. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1 (By similarity). Probably found in a ternary complex composed of DSCAM, PAK1 and RAC1. Interacts with DSCAM (via cytoplasmic domain); the interaction is direct and enhanced in presence of RAC1. Interacts with SCRIB. Interacts with PDPK1. Interacts (via kinase domain) with RAF1. Interacts with NCK1 and NCK2. Interacts with TBCB. Interacts with CRIPAK. Interacts with BRSK2 (By similarity). Interacts with SNAI1. Interacts with CIB1 isoform 2. Interacts with CIB1 (via N-terminal region); the interaction is direct, promotes PAK1 activity and occurs in a calcium-dependent manner. Interacts with INPP5K.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
文献引用
Application: WB Species: human Sample: HUVECs
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