产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3420, RRID:AB_2834862.
展开/折叠
C80703; cytoplasmic 3; D8Ertd281e; NF AT4; NF ATc3; NF-AT4; NF-ATc3; NFAC3_HUMAN; NFATc3; NFATx; Nuclear factor of activated T cells, cytoplasmic 3; nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3; Nuclear factor of activated T-cells; T cell transcription factor NFAT4; T-cell transcription factor NFAT4;
抗原和靶标
Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
- Q12968 NFAC3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVDPPPSTLTTPLCLPHHGLPSHSSVLSPSFQLQSHKNYEGTCEIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHLYLPLEPSYRESSLSPSPASSISSRSWFSDASSCESLSHIYDDVDSELNEAAARFTLGSPLTSPGGSPGGCPGEETWHQQYGLGHSLSPRQSPCHSPRSSVTDENWLSPRPASGPSSRPTSPCGKRRHSSAEVCYAGSLSPHHSPVPSPGHSPRGSVTEDTWLNASVHGGSGLGPAVFPFQYCVETDIPLKTRKTSEDQAAILPGKLELCSDDQGSLSPARETSIDDGLGSQYPLKKDSCGDQFLSVPSPFTWSKPKPGHTPIFRTSSLPPLDWPLPAHFGQCELKIEVQPKTHHRAHYETEGSRGAVKASTGGHPVVKLLGYNEKPINLQMFIGTADDRYLRPHAFYQVHRITGKTVATASQEIIIASTKVLEIPLLPENNMSASIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHIPQPSGKVLSLQIASIPVECSQRSAQELPHIEKYSINSCSVNGGHEMVVTGSNFLPESKIIFLEKGQDGRPQWEVEGKIIREKCQGAHIVLEVPPYHNPAVTAAVQVHFYLCNGKRKKSQSQRFTYTPVLMKQEHREEIDLSSVPSLPVPHPAQTQRPSSDSGCSHDSVLSGQRSLICSIPQTYASMVTSSHLPQLQCRDESVSKEQHMIPSPIVHQPFQVTPTPPVGSSYQPMQTNVVYNGPTCLPINAASSQEFDSVLFQQDATLSGLVNLGCQPLSSIPFHSSNSGSTGHLLAHTPHSVHTLPHLQSMGYHCSNTGQRSLSSPVADQITGQPSSQLQPITYGPSHSGSATTASPAASHPLASSPLSGPPSPQLQPMPYQSPSSGTASSPSPATRMHSGQHSTQAQSTGQGGLSAPSSLICHSLCDPASFPPDGATVSIKPEPEDREPNFATIGLQDITLDDVNEIIGRDMSQISVSQGAGVSRQAPLPSPESLDLGRSDGL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q12968 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S82 | Phosphorylation | Uniprot | |
| Y86 | Phosphorylation | Uniprot | |
| T89 | Phosphorylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| Y150 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | P45984 (MAPK9) | Uniprot |
| S165 | Phosphorylation | P45984 (MAPK9) | Uniprot |
| S168 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S172 | Phosphorylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| S181 | Phosphorylation | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| T204 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| S207 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| T210 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| S211 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| S215 | Phosphorylation | P48729 (CSNK1A1) | Uniprot |
| S236 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| T268 | Phosphorylation | Uniprot | |
| S269 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| K342 | Ubiquitination | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| K354 | Ubiquitination | Uniprot | |
| S364 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| T371 | Phosphorylation | Uniprot | |
| S372 | Phosphorylation | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K403 | Ubiquitination | Uniprot | |
| K405 | Ubiquitination | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| K457 | Ubiquitination | Uniprot | |
| K467 | Ubiquitination | Uniprot | |
| K542 | Ubiquitination | Uniprot | |
| S546 | Phosphorylation | Uniprot | |
| K552 | Ubiquitination | Uniprot | |
| K630 | Ubiquitination | Uniprot | |
| K636 | Ubiquitination | Uniprot | |
| K649 | Ubiquitination | Uniprot | |
| S730 | Phosphorylation | Uniprot | |
| S731 | Phosphorylation | Uniprot | |
| S742 | Phosphorylation | Uniprot | |
| Y884 | Phosphorylation | Uniprot | |
| S1045 | Phosphorylation | Uniprot | |
| S1048 | Phosphorylation | Uniprot | |
| S1063 | Phosphorylation | Uniprot | |
| S1066 | Phosphorylation | Uniprot |
研究背景
Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2. Along with NFATC4, involved in embryonic heart development (By similarity).
Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.
Cytoplasm. Nucleus.
Note: Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.
Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers.
Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.
研究领域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.