SNAP29 Antibody - #DF12743

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产品: SNAP29 抗体
货号: DF12743
描述: Rabbit polyclonal antibody to SNAP29
应用: WB
反应: Human, Mouse, Rat
预测: Bovine, Rabbit
分子量: 29 kDa; 29kD(Calculated).
蛋白号: O95721
RRID: AB_2845704

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(83%), Rabbit(92%)
克隆:
Polyclonal
特异性:
SNAP29 Antibody detects endogenous levels of total SNAP29.
RRID:
AB_2845704
引用格式: Affinity Biosciences Cat# DF12743, RRID:AB_2845704.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CEDNIK; FLJ21051; SNAP 29; SNAP-29; SNAP29; SNP29_HUMAN; Soluble 29 kDa NSF attachment protein; Synaptosomal associated protein 29; Synaptosomal associated protein 29kDa; Synaptosomal-associated protein 29; Vesicle membrane fusion protein SNAP 29; Vesicle membrane fusion protein SNAP29; Vesicle-membrane fusion protein SNAP-29;

抗原和靶标

免疫原:
Uniprot:

O95721(SNP29_HUMAN) >>Visit HPA database.

基因/基因ID:
SNAP29(9342)
表达:
O95721 SNP29_HUMAN:

Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.

序列:
MSAYPKSYNPFDDDGEDEGARPAPWRDARDLPDGPDAPADRQQYLRQEVLRRAEATAASTSRSLALMYESEKVGVASSEELARQRGVLERTEKMVDKMDQDLKISQKHINSIKSVFGGLVNYFKSKPVETPPEQNGTLTSQPNNRLKEAISTSKEQEAKYQASHPNLRKLDDTDPVPRGAGSAMSTDAYPKNPHLRAYHQKIDSNLDELSMGLGRLKDIALGMQTEIEEQDDILDRLTTKVDKLDVNIKSTERKVRQL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
92
Bovine
83
Pig
67
Dog
67
Horse
56
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O95721 作为底物

Site PTM Type Enzyme
K6 Ubiquitination
S7 Phosphorylation
Y8 Phosphorylation
R29 Methylation
R41 Methylation
Y44 Phosphorylation
T56 Phosphorylation
S59 Phosphorylation
T60 Phosphorylation
S61 Phosphorylation
S63 Phosphorylation
Y68 Phosphorylation
S77 Phosphorylation
S78 Phosphorylation
K103 Acetylation
K103 Ubiquitination
S105 Phosphorylation
K107 Ubiquitination
S111 Phosphorylation
K113 Ubiquitination
S114 Phosphorylation
Y122 Phosphorylation
K126 Ubiquitination
T130 Phosphorylation
T137 Phosphorylation
K147 Ubiquitination
S153 Phosphorylation
K154 Acetylation
K154 Ubiquitination
K159 Ubiquitination
Y160 Phosphorylation
S163 Phosphorylation
K169 Ubiquitination
S182 Phosphorylation
S185 Phosphorylation
T186 Phosphorylation
Y189 Phosphorylation
K191 Ubiquitination
S204 Phosphorylation
S210 Phosphorylation
K240 Ubiquitination
K249 Ubiquitination

研究背景

功能:

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Plays also a role in ciliogenesis by regulating membrane fusions.

细胞定位:

Cytoplasm. Golgi apparatus membrane>Peripheral membrane protein. Cytoplasmic vesicle>Autophagosome membrane>Peripheral membrane protein. Cell projection>Cilium membrane>Peripheral membrane protein.
Note: Appears to be mostly membrane-bound, probably via interaction with syntaxins, but a significant portion is cytoplasmic. Localizes to the ciliary pocket from where the cilium protrudes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Found in brain, heart, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.

亚基结构:

Forms a SNARE complex, composed of VAMP8, SNAP29 and STX17, involved in fusion of autophagosome with lysosome. Interacts with multiple syntaxins including STX6 (By similarity). Interacts with EIPR1.

蛋白家族:

Belongs to the SNAP-25 family.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

文献引用

1). mTOR deletion ameliorates CD4+ T cell apoptosis during sepsis by improving autophagosome-lysosome fusion. APOPTOSIS (PubMed: 35435531) [IF=7.2]

Application: WB    Species: Mice    Sample: CD4 + T cells

Fig. 3 Transcription factor EB (TFEB) phosphorylation and SNARE expression in septic mice. (A, a–e) Protein levels of phospho (p)-TFEB, syntaxin 17 (STX17), vesicle associated membrane protein 8 (VAMP8), and synaptosomal-associated protein 29 (SNAP29) in CD4 + T cells were quantified by western blotting in wild-type (WT) sham-treated mice, WT CLP mice, mammalian target of rapamycin (mTOR) knockout via Cre expression under the control of lymphocyte-specific protein tyrosine kinase (Lck) (Lck-mTOR) CLP mice, and tuberous sclerosis complex 1 (TSC1) knockout (Lck-TSC1) CLP mice. The amount of each protein level was normalized to that of β-actin. Means ± standard deviations (SDs) of four mice per group are shown. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Application: WB    Species: mouse    Sample: CD4+T cells

Fig. 3| Transcription factor EB (TFEB) phosphorylation and SNARE expression in septic mice. (A, a–e) Protein levels of phospho (p)-TFEB, syntaxin 17 (STX17), vesicle associated membrane protein 8 (VAMP8), and synaptosomal-associated protein 29 (SNAP29) in CD4+T cells were quantifed by western blotting in wild-type (WT)sham-treated mice, WT CLP mice, mammalian target of rapamycin(mTOR) knockout via Cre expression under the control of lymphocyte-specifc protein tyrosine kinase (Lck) (Lck-mTOR) CLP mice,and tuberous sclerosis complex 1 (TSC1) knockout (Lck-TSC1) CLP mice.
2). Improving Lipophagy by Restoring Rab7 Cycle: Protective Effects of Quercetin on Ethanol-Induced Liver Steatosis. Nutrients (PubMed: 35277017) [IF=5.9]

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