PSPH Antibody - #DF12711

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产品: PSPH 抗体
货号: DF12711
描述: Rabbit polyclonal antibody to PSPH
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: P78330
RRID: AB_2845672

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
PSPH Antibody detects endogenous levels of total PSPH.
RRID:
AB_2845672
引用格式: Affinity Biosciences Cat# DF12711, RRID:AB_2845672.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

EC 3.1.3.3; L 3 phosphoserine phosphatase; L-3-phosphoserine phosphatase; O phosphoserine phosphohydrolase; O-phosphoserine phosphohydrolase; Phosphoserine phosphatase; Phosphoserine phosphatase deficiency, included; PSP; PSPase; Psph; PSPHD; SERB_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human PSPH, corresponding to a region within the internal amino acids.

基因/基因ID:
PSPH(5723)

研究领域

· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

文献引用

1). Chemotherapy-induced macrophage CXCL7 expression drives tumor chemoresistance via the STAT1/PHGDH-serine metabolism axis and SAM paracrine feedback to M2 polarization. Cell death & disease, 2025 (PubMed: 40368902) [IF=8.1]
2). Serine Metabolism Regulates the Replicative Senescence of Human Dental Pulp Cells through Histone Methylation. Current issues in molecular biology, 2024 (PubMed: 38666909) [IF=2.8]

Application: WB    Species: human    Sample: hDPCs

Figure 2. PHGDH is involved in the cellular senescence of hDPCs. (a) Analysis of the transcription of PHGDH, PSAT1, and PSPH in different passages (P5 and P12) of hDPCs by RT-qPCR. (b) Analysis of PHGDH, PSAT1, and PSPH expression in different passages (P5 and P12) of hDPCs by western blot. (c) Young hDPCs (P5) were treated with different concentrations of NCT—503 and CBR—5884 for 48 h, and cell viability was determined by CCK8 assay. (d) Effects of NCT—503 (20 μM) and CBR—5884 (30 μM) treatment for 48 h on young hDPCs (P5) were determined by SA-β-gal staining, Ki67 staining, and yH2AX staining. In SA-β-gal staining, blue-stained cells are positive cells. In Ki67 staining and yH2AX staining, the blue color in the nucleus shows DAPI staining, the green color in the nucleus shows Ki67 or yH2AX staining. (e) Transcription of LMNB1 and P21 in hDPCs (P5) treated with NCT—503 (20 μM) and CBR—5884 (30 μM) for 48 h was analyzed by RT-qPCR. (f) Crystal violet staining determined colony formation at 7 days after continuous treatment with NCT—503 (20 μM) and 2 days of CBR—5884 (30 μM) treatment. Data were expressed as means ± SD, n = 3 independent experiments. * p < 0.05.

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