产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12656, RRID:AB_2845618.
展开/折叠
Activator recruited cofactor 240 kDa component; Activator-recruited cofactor 240 kDa component; ARC240; CAG repeat protein 45; CAGH45; HOPA; KIAA0192; MED12; MED12_HUMAN; Mediator complex subunit 12; Mediator of RNA polymerase II transcription subunit 12; OPA containing protein; OPA-containing protein; Thyroid hormone receptor associated protein complex 230 kDa component; Thyroid hormone receptor-associated protein complex 230 kDa component; TNRC11; Trap230; Trinucleotide repeat containing gene 11 protein; Trinucleotide repeat-containing gene 11 protein;
抗原和靶标
- Q93074 MED12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAFGILSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVSFNPAKISSNFSSIIAEKLRCNTLPDTGRRKPQVNQKDNFWLVTARSQSAINTWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAISETKVKKRHVDPFMEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEVAIRQWDYTEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVISAQSTSTLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSIASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPADDPEHKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPPKEKIEGTLGVLYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKNKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVTGGTEELPEEEGGGGSGGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILQNLDQWTMRQSSLELQLMIKQTPNNEMNSLLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVHQIVNNWRDDQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAMLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLAKKLQKELGERQSDSLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWDLFEGLKPSAPLSWGWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPTLLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKTEDYGMGPGRSGPYGVTVPPDLLHHPNPGSITHLNYRQGSIGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMTGVMGLEPSSYKTSVYRQQQPAVPQGQRLRQQLQQSQGMLGQSSVHQMTPSSSYGLQTSQGYTPYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMISTMTPMSAQGVQAGVRSTAILPEQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQILRQQQQQQQQQQQQQQQQQQQQQQQQQQHQQQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q93074 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K32 | Ubiquitination | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| Y166 | Phosphorylation | Uniprot | |
| T302 | Phosphorylation | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| S476 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| K515 | Ubiquitination | Uniprot | |
| K527 | Ubiquitination | Uniprot | |
| K531 | Ubiquitination | Uniprot | |
| S635 | Phosphorylation | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| S688 | Phosphorylation | Uniprot | |
| S698 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Uniprot | |
| K762 | Ubiquitination | Uniprot | |
| K773 | Ubiquitination | Uniprot | |
| K776 | Ubiquitination | Uniprot | |
| K780 | Ubiquitination | Uniprot | |
| K785 | Ubiquitination | Uniprot | |
| K814 | Ubiquitination | Uniprot | |
| K832 | Ubiquitination | Uniprot | |
| K988 | Ubiquitination | Uniprot | |
| K999 | Acetylation | Uniprot | |
| K999 | Ubiquitination | Uniprot | |
| K1001 | Ubiquitination | Uniprot | |
| K1181 | Ubiquitination | Uniprot | |
| K1194 | Ubiquitination | Uniprot | |
| S1258 | Phosphorylation | Uniprot | |
| S1269 | Phosphorylation | Uniprot | |
| K1298 | Ubiquitination | Uniprot | |
| K1414 | Ubiquitination | Uniprot | |
| K1434 | Ubiquitination | Uniprot | |
| K1445 | Ubiquitination | Uniprot | |
| K1453 | Ubiquitination | Uniprot | |
| K1471 | Ubiquitination | Uniprot | |
| K1524 | Ubiquitination | Uniprot | |
| K1534 | Ubiquitination | Uniprot | |
| K1619 | Ubiquitination | Uniprot | |
| K1622 | Ubiquitination | Uniprot | |
| K1634 | Ubiquitination | Uniprot | |
| K1643 | Ubiquitination | Uniprot | |
| K1661 | Ubiquitination | Uniprot | |
| K1664 | Ubiquitination | Uniprot | |
| K1673 | Ubiquitination | Uniprot | |
| K1674 | Ubiquitination | Uniprot | |
| K1682 | Ubiquitination | Uniprot | |
| K1761 | Acetylation | Uniprot | |
| K1761 | Ubiquitination | Uniprot | |
| S1778 | Phosphorylation | Uniprot | |
| T1779 | Phosphorylation | Uniprot | |
| K1798 | Acetylation | Uniprot | |
| K1798 | Ubiquitination | Uniprot | |
| S1838 | Phosphorylation | Uniprot | |
| R1854 | Methylation | Uniprot | |
| R1859 | Methylation | Uniprot | |
| R1862 | Methylation | Uniprot | |
| R1871 | Methylation | Uniprot | |
| R1899 | Methylation | Uniprot | |
| R1910 | Methylation | Uniprot | |
| R1912 | Methylation | Uniprot | |
| R1994 | Methylation | Uniprot | |
| R2015 | Methylation | Uniprot | |
| S2163 | Phosphorylation | Uniprot |
研究背景
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
Nucleus.
Ubiquitous.
Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Also interacts with CTNNB1 and GLI3.
Belongs to the Mediator complex subunit 12 family.
研究领域
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
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