DARS2 Antibody - #DF12593

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产品: DARS2 抗体
货号: DF12593
描述: Rabbit polyclonal antibody to DARS2
应用: WB IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
蛋白号: Q6PI48
RRID: AB_2845555

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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实验方案
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
DARS2 Antibody detects endogenous levels of total DARS2.
RRID:
AB_2845555
引用格式: Affinity Biosciences Cat# DF12593, RRID:AB_2845555.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Aspartate tRNA ligase 2 mitochondrial; Aspartate tRNA ligase; Aspartate tRNA ligase mitochondrial; Aspartate--tRNA ligase; Aspartyl tRNA synthetase 2 (mitochondrial); Aspartyl tRNA synthetase mitochondrial; Aspartyl-tRNA synthetase; AspRS; DARS2; FLJ10514; LBSL; mitochondrial; MT ASPRS; RP3 383J4.2; SYDM_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human DARS2, corresponding to a region within the internal amino acids.

基因/基因ID:
DARS2(55157)

研究领域

· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.

文献引用

1). DARS2 overexpression is associated with PET/CT metabolic parameters and affects glycolytic activity in lung adenocarcinoma. Journal of translational medicine, 2023 (PubMed: 37626419) [IF=7.4]

Application: IHC    Species: human    Sample:

Fig. 2 Immunohistochemistry staining of lung adenocarcinoma samples. A, B Representative DARS2 IHC images and scores in adjacent normal tissue and LUAD tissue (adjacent = 62, tumor = 62). C DARS2’s diagnostic value in LUAD patients shown in the ROC curve. D, E Representative IHC images and DARS2 scores in different pathological stages of LUAD (I = 38, II = 11, III = 13). ns means not significant; ***p 

Application: WB    Species: human    Sample:

Fig. 3 Interference DARS2 expression inhibited the proliferation of LUAD cells. A qRT-PCR verified the interference efficiency of 6 different sequence siRNAs. B, C WB verified the interference efficiency of 6 different sequences of siRNA. D Representative images of EdU staining. E Quantitative analysis of EdU staining. F CCK-8 assays were performed to evaluate the LUAD cell’s viability and growth. ns means not significant; *p 

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