ANGPTL2 Antibody - #DF12557

Figure 5. miR-221 impairs angiogenesis in endothelial cells by targeting Angptl2. (A) The sequences of sus-miR-221-3p and its predicted binding sites position of Angptl2 3′UTR using miRbase, miRanda, and TargetScan. (B) qPCR was performed to detect the mRNA expression of Angptl2 after transfection with miR-221 inhibitor or miR-221 mimic. (C) Western blotting was performed to detect the protein expression of Angptl2 after transfection with miR-221 inhibitor or miR-221 mimic. (D) A dual-luciferase reporter assay was performed in 293T cells. (E) Angptl2 overexpression attenuated the suppressive function of miR-221-mediated angiogenesis in endothelial cells. (F) qPCR was performed to detect the mRNA expression of Angptl2 after incubation with exosomes from normal or obese sows. (G) Western blotting was performed to detect the protein expression of Angptl2 after incubation with exosomes from normal or obese sows. Values are means ± SD (n = 6 preparative replicates). * p < 0.05.

Fig. 5 |The protein levels of Angptl2, Foxo1, NF-κB, p-NF-κB and CCR2 were measured by Western blot analysis and normalized by GAPDH expression. Data are presented as mean ± SD from eight rats. **P < 0.01,* P < 0.05.n = 8/group