TREM2 Antibody - #DF12529

Fig. 1: TREM2 deficiency impaired cognitive function in PD mice. A Representative image of TREM2 (red) and IBA1 (green) immunofluorescence staining in hippocampal tissue sections of the PD and control specimens. Scale bar, 50μm. B Representative image of TREM2 (red) and IBA1 (green) immunofluorescence staining in hippocampus in C57BL/6 mice and A53T mice. Scale bar, 50 μm. C Quantification of number of TREM2+ IBA1+ cells in PD specimens and control specimens (n = 3, unpaired Student’s t test) and TREM2+ IBA1+ cells in C57BL/6 mice and A53T mice (n = 3, unpaired Student’s t test). D Recognition Index was calculated in NORT (n = 9, one-way ANOVA and Šídák’s multiple comparisons test). E Representative image showing the procedure and swimming paths obtained from each group in the acquisition phase in MWM. F Time spent in the target quadrant (s) in the acquisition phase in MWM (n = 9, one-way ANOVA and Šídák’s multiple comparisons test). G Representative image showing the paths obtained from each group in the acquisition phase in Open field test. Line chart showing the average escape latencies in each group in the space exploring phase in MWM (n = 9, two-way ANOVA and Tukey’s multiple comparisons test). Column charts showing. The error bars represent the ± SDs. **p 

Fig. 5: Knockdown of TREM2 aggravated α-Syn-induced lysosomal dysfunction in BV-2 cells via the ERK1/2/TFEB pathway. A Representative images of western blotting analysis of TREM2, ERK1/2, and p-ERK1/2 expressions in TREM2KD BV-2 cells and NCKD BV-2 cells. B Representative images of western blotting analysis of Total-TFEB, Nuclear-TFEB, and Cytoplasm-TFEB expressions in TREM2KD BV-2 cells and NCKD BV-2 cells. C Quantification of TREM2, ERK1/2, p-ERK1/2, Total-TFEB, Nuclear-TFEB, and Cytoplasm-TFEB expressions proteins relative expression (n = 3, one-way ANOVA and Šídák’s multiple comparisons test). D Representative images of TFEB (red) immunofluorescence staining in TREM2KD BV-2 cells and NCKD BV-2 cells. Scale bar, 10 μm. E Quantification of Nuclear- Cytoplasm TFEB Fluorescence ratio in TREM2KD BV-2 cells and NCKD BV-2 cells (n = 3, one-way ANOVA and Šídák’s multiple comparisons test). F Representative images of DQ-BSA (red), Lysotracker (red), and CathB (red) in TREM2KD BV-2 cells and NCKD BV-2 cells. Scale bar, 10μm. G Representative images of α-Syn (red) and Lamp1 (green) immunofluorescence staining in TREM2KD BV-2 cells and NCKD BV-2 cells. Scale bar, 5 μm. H Quantification of integrated density of DQ-BSA, Lysotracker, and CathB in TREM2KD BV-2 cells and NCKD BV-2 cells (n = 3, one-way ANOVA and Šídák’s multiple comparisons test). I Quantification of MCC of α-Syn and Lamp1 in TREM2KD BV-2 cells and NCKD BV-2 cells (n = 3, unpaired Student’s t test) and mean Fluorescence intensity of α-Syn in TREM2KD BV-2 cells and NCKD BV-2 cells (n = 3, unpaired Student’s t test). The error bars represent ± SDs. *p 

FIGURE 9 | Ameliorate effects of Ganodenic acid A by regulating sphingolipid metabolism in 3 × Tg-AD mice. (A) Experimental procedure in 3 × Tg-AD mice;(B) The AD biomarkers of p-Tau, Aβ, APOE, TREM2, CD33, the inflammatory cytokines of TNF-α and NF-κB p65 and the autophagy level of LC3A/B were measured in brain tissues

Figure 2 RT-qPCR and Western blotting analyses of TREM2 siRNA transfection efficiency. (a) Relative expression levels of TREM2 mRNA. (b) Expression levels of TREM2 protein. aP < 0.05 vs. control, bP < 0.05 vs. siRNA-NC, mean ± SEM, n = 3.

Figure 2:| RT-qPCR and Western blotting analyses of TREM2 siRNA transfection efficiency. (a) Relative expression levels of TREM2 mRNA. (b) Expression levels of TREM2 protein.

Figure 4. |rSj‑Cys inhibits the expression of osteoclastogenesis‑related genes and proteins. RAW264.7 cells were co‑stimulated with M‑CSF (25 ng/ml) and RANKL (30 ng/ml) and then treated with or without rSj‑Cys (0.3 µM) for different time periods (24, 48 or 72 h). The expression levels of genes and proteins associated with osteoclast phenotype markers (CTSK, TRAP, ITGB3) and cell surface receptors of osteoclast precursors (RANK, OSCAR, TREM‑2)were determined by (A) reverse transcription‑quantitative PCR and (B) western blot analysis.