产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3411, RRID:AB_2834838.
展开/折叠
CAD; CALD 1; CALD1; CALD1_HUMAN; Caldesmon 1; Caldesmon 1 Isoform 1; Caldesmon 1 Isoform 2; Caldesmon 1 Isoform 3; Caldesmon 1 Isoform 4; Caldesmon 1 Isoform 5; Caldesmon; Caldesmon1; CDM; H CAD; HCAD; L CAD; LCAD; MGC21352; NAG22;
抗原和靶标
High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
- Q05682 CALD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDDFERRRELRRQKREEMRLEAERIAYQRNDDDEEEAARERRRRARQERLRQKQEEESLGQVTDQVEVNAQNSVPDEEAKTTTTNTQVEGDDEAAFLERLARREERRQKRLQEALERQKEFDPTITDASLSLPSRRMQNDTAENETTEKEEKSESRQERYEIEETETVTKSYQKNDWRDAEENKKEDKEKEEEEEEKPKRGSIGENQVEVMVEEKTTESQEETVVMSLKNGQISSEEPKQEEEREQGSDEISHHEKMEEEDKERAEAERARLEAEERERIKAEQDKKIADERARIEAEEKAAAQERERREAEERERMREEEKRAAEERQRIKEEEKRAAEERQRIKEEEKRAAEERQRIKEEEKRAAEERQRARAEEEEKAKVEEQKRNKQLEEKKHAMQETKIKGEKVEQKIEGKWVNEKKAQEDKLQTAVLKKQGEEKGTKVQAKREKLQEDKPTFKKEEIKDEKIKKDKEPKEEVKSFMDRKKGFTEVKSQNGEFMTHKLKHTENTFSRPGGRASVDTKEAEGAPQVEAGKRLEELRRRRGETESEEFEKLKQKQQEAALELEELKKKREERRKVLEEEEQRRKQEEADRKLREEEEKRRLKEEIERRRAEAAEKRQKMPEDGLSDDKKPFKCFTPKGSSLKIEERAEFLNKSVQKSSGVKSTHQAAIVSKIDSRLEQYTSAIEGTKSAKPTKPAASDLPVPAEGVRNIKSMWEKGNVFSSPTAAGTPNKETAGLKVGVSSRINEWLTKTPDGNKSPAPKPSDLRPGDVSSKRNLWEKQSVDKVTSPTKV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q05682 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y27 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | Q15746 (MYLK) | Uniprot |
| T83 | Phosphorylation | Uniprot | |
| T84 | Phosphorylation | Uniprot | |
| T86 | Phosphorylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| S131 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | Uniprot | |
| Y160 | Phosphorylation | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| Y172 | Phosphorylation | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K197 | Sumoylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| T402 | Phosphorylation | Uniprot | |
| K427 | Acetylation | Uniprot | |
| K479 | Ubiquitination | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| T506 | Phosphorylation | Uniprot | |
| T509 | Phosphorylation | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| T521 | Phosphorylation | Uniprot | |
| K534 | Acetylation | Uniprot | |
| T546 | Phosphorylation | Uniprot | |
| S548 | Phosphorylation | Uniprot | |
| K569 | Ubiquitination | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| T638 | Phosphorylation | Uniprot | |
| K640 | Acetylation | Uniprot | |
| S642 | Phosphorylation | Uniprot | |
| S643 | Phosphorylation | P17252 (PRKCA) , Q9UQM7 (CAMK2A) | Uniprot |
| K645 | Acetylation | Uniprot | |
| K645 | Sumoylation | Uniprot | |
| K655 | Acetylation | Uniprot | |
| K655 | Ubiquitination | Uniprot | |
| S656 | Phosphorylation | P17252 (PRKCA) , Q9UQM7 (CAMK2A) | Uniprot |
| S660 | Phosphorylation | Uniprot | |
| S661 | Phosphorylation | Uniprot | |
| T666 | Phosphorylation | Q15746 (MYLK) | Uniprot |
| S673 | Phosphorylation | Uniprot | |
| K674 | Ubiquitination | Uniprot | |
| S677 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| Y682 | Phosphorylation | Uniprot | |
| S684 | Phosphorylation | Uniprot | |
| K690 | Ubiquitination | Uniprot | |
| S691 | Phosphorylation | Uniprot | |
| T695 | Phosphorylation | Uniprot | |
| S700 | Phosphorylation | Q15746 (MYLK) | Uniprot |
| K713 | Ubiquitination | Uniprot | |
| S714 | Phosphorylation | O75914 (PAK3) , Q13177 (PAK2) | Uniprot |
| K718 | Ubiquitination | Uniprot | |
| S723 | Phosphorylation | Uniprot | |
| S724 | Phosphorylation | Uniprot | |
| T726 | Phosphorylation | Uniprot | |
| T730 | Phosphorylation | P24941 (CDK2) , Q00534 (CDK6) | Uniprot |
| K733 | Acetylation | Uniprot | |
| K733 | Ubiquitination | Uniprot | |
| T735 | Phosphorylation | Q15746 (MYLK) | Uniprot |
| S744 | Phosphorylation | Q15746 (MYLK) , Q13177 (PAK2) , O75914 (PAK3) | Uniprot |
| K752 | Acetylation | Uniprot | |
| T753 | Phosphorylation | Uniprot | |
| K758 | Acetylation | Uniprot | |
| S759 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S765 | Phosphorylation | Uniprot | |
| S773 | Phosphorylation | Uniprot | |
| K775 | Methylation | Uniprot | |
| K775 | Ubiquitination | Uniprot | |
| S783 | Phosphorylation | Uniprot | |
| T788 | Phosphorylation | Uniprot | |
| S789 | Phosphorylation | P27361 (MAPK3) , Q00534 (CDK6) , P28482 (MAPK1) | Uniprot |
| T791 | Phosphorylation | Uniprot |
研究背景
Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).
In non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. CDK1-mediated phosphorylation promotes Schwann cell migration during peripheral nerve regeneration (By similarity).
Cytoplasm>Cytoskeleton. Cytoplasm>Myofibril. Cytoplasm>Cytoskeleton>Stress fiber.
Note: On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).
High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form.
Belongs to the caldesmon family.
研究领域
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
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