Phospho-FAK (Tyr861) Antibody - #AF3399
产品: | 磷酸化 FAK (Tyr861) 抗体 |
货号: | AF3399 |
描述: | Rabbit polyclonal antibody to Phospho-FAK (Tyr861) |
应用: | WB IF/ICC |
反应: | Human, Mouse, Rat |
预测: | Pig, Horse, Sheep, Dog, Chicken, Xenopus |
分子量: | 125kDa; 119kD(Calculated). |
蛋白号: | Q05397 |
RRID: | AB_2834830 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3399, RRID:AB_2834830.
展开/折叠
FADK 1; FADK; FAK related non kinase polypeptide; FAK1; FAK1_HUMAN; Focal adhesion kinase 1; Focal adhesion Kinase; Focal adhesion kinase isoform FAK Del33; Focal adhesion kinase related nonkinase; FRNK; p125FAK; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; Protein tyrosine kinase 2; Protein-tyrosine kinase 2; Ptk2; PTK2 protein tyrosine kinase 2;
抗原和靶标
Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous.
- Q05397 FAK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAAYLDPNLNHTPNSSTKTHLGTGMERSPGAMERVLKVFHYFESNSEPTTWASIIRHGDATDVRGIIQKIVDSHKVKHVACYGFRLSHLRSEEVHWLHVDMGVSSVREKYELAHPPEEWKYELRIRYLPKGFLNQFTEDKPTLNFFYQQVKSDYMLEIADQVDQEIALKLGCLEIRRSYWEMRGNALEKKSNYEVLEKDVGLKRFFPKSLLDSVKAKTLRKLIQQTFRQFANLNREESILKFFEILSPVYRFDKECFKCALGSSWIISVELAIGPEEGISYLTDKGCNPTHLADFTQVQTIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMADLIDGYCRLVNGTSQSFIIRPQKEGERALPSIPKLANSEKQGMRTHAVSVSETDDYAEIIDEEDTYTMPSTRDYEIQRERIELGRCIGEGQFGDVHQGIYMSPENPALAVAIKTCKNCTSDSVREKFLQEALTMRQFDHPHIVKLIGVITENPVWIIMELCTLGELRSFLQVRKYSLDLASLILYAYQLSTALAYLESKRFVHRDIAARNVLVSSNDCVKLGDFGLSRYMEDSTYYKASKGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKAQQEERMRMESRRQATVSWDSGGSDEAPPKPSRPGYPSPRSSEGFYPSPQHMVQTNHYQVSGYPGSHGITAMAGSIYPGQASLLDQTDSWNHRPQEIAMWQPNVEDSTVLDLRGIGQVLPTHLMEERLIRQQQEMEEDQRWLEKEERFLKPDVRLSRGSIDREDGSLQGPIGNQHIYQPVGKPDPAAPPKKPPRPGAPGHLGSLASLSSPADSYNEGVKLQPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDETIPLLPASTHREIEMAQKLLNSDLGELINKMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKNLLDVIDQARLKMLGQTRPH
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q05397 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
A2 | Acetylation | Uniprot | |
Y5 | Phosphorylation | P09619 (PDGFRB) , P08581 (MET) , P00533 (EGFR) | Uniprot |
T13 | Phosphorylation | Uniprot | |
S16 | Phosphorylation | Uniprot | |
T20 | Phosphorylation | Uniprot | |
T24 | Phosphorylation | Uniprot | |
S29 | Phosphorylation | Uniprot | |
R65 | Methylation | Uniprot | |
K70 | Ubiquitination | Uniprot | |
Y148 | Phosphorylation | Uniprot | |
Y155 | Phosphorylation | Uniprot | |
Y194 | Phosphorylation | P09619 (PDGFRB) , P12931 (SRC) , P00533 (EGFR) , P51813 (BMX) , P08581 (MET) | Uniprot |
K199 | Ubiquitination | Uniprot | |
K216 | Ubiquitination | Uniprot | |
S248 | Phosphorylation | Uniprot | |
Y347 | Phosphorylation | Uniprot | |
K364 | Ubiquitination | Uniprot | |
S372 | Phosphorylation | Uniprot | |
T386 | Phosphorylation | Uniprot | |
S390 | Phosphorylation | Uniprot | |
S392 | Phosphorylation | Uniprot | |
T394 | Phosphorylation | Uniprot | |
Y397 | Phosphorylation | P12931 (SRC) , Q05397 (PTK2) , P08581 (MET) , P46108 (CRK) , A0A059VC25 (FAK) , P09769 (FGR) | Uniprot |
T406 | Phosphorylation | Uniprot | |
Y407 | Phosphorylation | P08581 (MET) , Q05397 (PTK2) , Q14289 (PTK2B) , P12931 (SRC) | Uniprot |
T408 | Phosphorylation | Uniprot | |
Y441 | Phosphorylation | P51813 (BMX) , P12931 (SRC) | Uniprot |
S443 | Phosphorylation | Uniprot | |
T460 | Phosphorylation | Uniprot | |
S461 | Phosphorylation | Uniprot | |
S463 | Phosphorylation | Uniprot | |
K467 | Acetylation | Uniprot | |
T474 | Phosphorylation | Uniprot | |
T503 | Phosphorylation | Uniprot | |
S517 | Phosphorylation | Uniprot | |
S568 | Phosphorylation | Uniprot | |
Y570 | Phosphorylation | Uniprot | |
S574 | Phosphorylation | Uniprot | |
T575 | Phosphorylation | Uniprot | |
Y576 | Phosphorylation | P08581 (MET) , P06241 (FYN) , P07949 (RET) , Q05397 (PTK2) , A0A059VC25 (FAK) , P51813 (BMX) , P07947 (YES1) , P09769 (FGR) , P12931 (SRC) | Uniprot |
Y577 | Phosphorylation | P12931 (SRC) , A0A059VC25 (FAK) , P51813 (BMX) , P07949 (RET) , P08581 (MET) , Q05397 (PTK2) | Uniprot |
S580 | Phosphorylation | Uniprot | |
S677 | Phosphorylation | Uniprot | |
K684 | Ubiquitination | Uniprot | |
S695 | Phosphorylation | P31749 (AKT1) | Uniprot |
T700 | Phosphorylation | P31749 (AKT1) | Uniprot |
S702 | Phosphorylation | Uniprot | |
S705 | Phosphorylation | Uniprot | |
S708 | Phosphorylation | Uniprot | |
S716 | Phosphorylation | Uniprot | |
Y720 | Phosphorylation | Uniprot | |
S722 | Phosphorylation | P49840 (GSK3A) | Uniprot |
S726 | Phosphorylation | Uniprot | |
S732 | Phosphorylation | Q13464 (ROCK1) , Q00535 (CDK5) | Uniprot |
Y742 | Phosphorylation | P12931 (SRC) , P51813 (BMX) | Uniprot |
S759 | Phosphorylation | Uniprot | |
Y761 | Phosphorylation | Uniprot | |
T805 | Phosphorylation | Uniprot | |
S840 | Phosphorylation | Uniprot | |
S843 | Phosphorylation | Q9UQM7 (CAMK2A) | Uniprot |
S850 | Phosphorylation | Uniprot | |
Y861 | Phosphorylation | Q05397 (PTK2) , P07949 (RET) , P08581 (MET) , Q13882 (PTK6) , P16591 (FER) , P12931 (SRC) , P51813 (BMX) , P07947 (YES1) | Uniprot |
S887 | Phosphorylation | Uniprot | |
S892 | Phosphorylation | Uniprot | |
S893 | Phosphorylation | Uniprot | |
Y898 | Phosphorylation | P51813 (BMX) , P12931 (SRC) | Uniprot |
S910 | Phosphorylation | Q00535 (CDK5) , P27361 (MAPK3) , P28482 (MAPK1) | Uniprot |
T914 | Phosphorylation | Uniprot | |
S920 | Phosphorylation | Uniprot | |
K923 | Ubiquitination | Uniprot | |
Y925 | Phosphorylation | P12931 (SRC) , P16591 (FER) , P09769 (FGR) , P51813 (BMX) , P07947 (YES1) , P07949 (RET) , P08581 (MET) | Uniprot |
T929 | Phosphorylation | Uniprot | |
T963 | Phosphorylation | Uniprot | |
T967 | Phosphorylation | Uniprot | |
T971 | Phosphorylation | Uniprot | |
K1000 | Ubiquitination | Uniprot | |
Y1007 | Phosphorylation | P12931 (SRC) , P51813 (BMX) | Uniprot |
K1017 | Acetylation | Uniprot |
翻译修饰 - Q05397 作为激酶
Substrate | Site | Source |
---|---|---|
O00401 (WASL) | Y256 | Uniprot |
O43707 (ACTN4) | Y4 | Uniprot |
O43707 (ACTN4) | Y31 | Uniprot |
O43707 (ACTN4) | Y265 | Uniprot |
O75962-1 (TRIO) | Y2796 | Uniprot |
P07949 (RET) | Y905 | Uniprot |
P12814-1 (ACTN1) | Y12 | Uniprot |
P23634-6 (ATP2B4) | Y1176 | Uniprot |
P26010 (ITGB7) | Y753 | Uniprot |
P26010 (ITGB7) | Y758 | Uniprot |
P29353 (SHC1) | Y315 | Uniprot |
P49023 (PXN) | Y31 | Uniprot |
P49023 (PXN) | Y118 | Uniprot |
P51813 (BMX) | Y40 | Uniprot |
P56945 (BCAR1) | Y410 | Uniprot |
P56945-1 (BCAR1) | Y664 | Uniprot |
P56945-1 (BCAR1) | Y666 | Uniprot |
P63000 (RAC1) | Y64 | Uniprot |
Q05397-1 (PTK2) | S390 | Uniprot |
Q05397 (PTK2) | Y397 | Uniprot |
Q05397 (PTK2) | Y407 | Uniprot |
Q05397 (PTK2) | Y576 | Uniprot |
Q05397 (PTK2) | Y577 | Uniprot |
Q05397-1 (PTK2) | S850 | Uniprot |
Q05397 (PTK2) | Y861 | Uniprot |
Q05397-1 (PTK2) | Y925 | Uniprot |
Q14451 (GRB7) | Y188 | Uniprot |
Q14451 (GRB7) | Y338 | Uniprot |
Q658W2 (DKFZp666O0110) | S412 | Uniprot |
Q658W2 (DKFZp666O0110) | Y419 | Uniprot |
Q658W2 (DKFZp666O0110) | S872 | Uniprot |
Q658W2 (DKFZp666O0110) | Y947 | Uniprot |
Q99961 (SH3GL1) | Y315 | Uniprot |
Q9H5V8 (CDCP1) | Y734 | Uniprot |
Q9H9S0 (NANOG) | Y35 | Uniprot |
Q9H9S0 (NANOG) | Y174 | Uniprot |
Q9Y2X7 (GIT1) | Y321 | Uniprot |
研究背景
Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
Phosphorylated on tyrosine residues upon activation, e.g. upon integrin signaling. Tyr-397 is the major autophosphorylation site, but other kinases can also phosphorylate this residue. Phosphorylation at Tyr-397 promotes interaction with SRC and SRC family members, leading to phosphorylation at Tyr-576, Tyr-577 and at additional tyrosine residues. FGR promotes phosphorylation at Tyr-397 and Tyr-576. FER promotes phosphorylation at Tyr-577, Tyr-861 and Tyr-925, even when cells are not adherent. Tyr-397, Tyr-576 and Ser-722 are phosphorylated only when cells are adherent. Phosphorylation at Tyr-397 is important for interaction with BMX, PIK3R1 and SHC1. Phosphorylation at Tyr-925 is important for interaction with GRB2. Dephosphorylated by PTPN11; PTPN11 is recruited to PTK2 via EPHA2 (tyrosine phosphorylated). Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly; this dephosphorylation could be catalyzed by PTPN11 and regulated by ZFYVE21. Phosphorylation on tyrosine residues is enhanced by NTN1 (By similarity).
Sumoylated; this enhances autophosphorylation.
Cell junction>Focal adhesion. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Cell cortex. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Nucleus.
Note: Constituent of focal adhesions. Detected at microtubules.
Detected in B and T-lymphocytes. Isoform 1 and isoform 6 are detected in lung fibroblasts (at protein level). Ubiquitous.
Interacts (via first Pro-rich region) with CAS family members (via SH3 domain), including BCAR1, BCAR3, CASS4 and NEDD9. Interacts with GIT1. Interacts with SORBS1. Interacts with ARHGEF28. Interacts with SHB. Interacts with PXN and TLN1. Interacts with STAT1. Interacts with DCC. Interacts with WASL. Interacts with ARHGEF7. Interacts with GRB2 and GRB7 (By similarity). Component of a complex that contains at least FER, CTTN and PTK2/FAK1. Interacts with BMX. Interacts with TGFB1I1. Interacts with STEAP4. Interacts with ZFYVE21. Interacts with ESR1. Interacts with PIK3R1 or PIK3R2. Interacts with SRC, FGR, FLT4 and RET. Interacts with EPHA2 in resting cells; activation of EPHA2 recruits PTPN11, leading to dephosphorylation of PTK2/FAK1 and dissociation of the complex. Interacts with EPHA1 (kinase activity-dependent). Interacts with CD4; this interaction requires the presence of HIV-1 gp120. Interacts with PIAS1. Interacts with ARHGAP26 and SHC1. Interacts with RB1CC1; this inhibits PTK2/FAK1 activity and activation of downstream signaling pathways. Interacts with P53/TP53 and MDM2. Interacts with LPXN (via LD motif 3). Interacts with MISP. Interacts with CIB1 isoform 2. Interacts with CD36. Interacts with EMP2; regulates PTK2 activation and localization. Interacts with DSCAM (By similarity).
The Pro-rich regions interact with the SH3 domain of CAS family members, such as BCAR1 and NEDD9, and with the GTPase activating protein ARHGAP26.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
文献引用
Application: WB Species: human Sample: MCF7 and MDA-MB-231 cells
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