Cleaved-Caspase 1 (Ala317), p10 Antibody - #AF4022

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产品: Cleaved-Caspase 1 (Ala317), p10 抗体
货号: AF4022
描述: Rabbit polyclonal antibody to Cleaved-Caspase 1 (Ala317), p10
应用: WB IHC IF/ICC
文献验证: WB, IHC, IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog
蛋白号: P29466
RRID: AB_2845464

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Cleaved-Caspase-1 (Ala317,p10) Antibody detects endogenous levels of fragment of activated Caspase-1 resulting from cleavage adjacent to Ala317.
RRID:
AB_2845464
引用格式: Affinity Biosciences Cat# AF4022, RRID:AB_2845464.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CASP-1; CASP1; CASP1_HUMAN; Caspase 1; Caspase-1 subunit p10; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1 beta converting enzyme; IL1B convertase; Interleukin 1 beta convertase; Interleukin 1B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45;

抗原和靶标

免疫原:

The antiserum was produced against synthesized peptide derived from human Caspase 1.

基因/基因ID:
CASP1(834)

研究领域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway.   (View pathway)

文献引用

1). An artificial metabzyme for tumour-cell-specific metabolic therapy. Nature nanotechnology, 2024 (PubMed: 39103450) [IF=38.1]
2). p66Shc Contributes to Liver Fibrosis through the Regulation of Mitochondrial Reactive Oxygen Species. Theranostics, 2019 (PubMed: 30867846) [IF=12.4]

Application: WB    Species: mouse    Sample: Liver

Figure 2.| p66Shc silencing attenuates liver fibrosis in mice. p66Shc silencing was induced via lentivirus delivered to C57BL/6 mice exposed to CCl4 (2 ml/kg). (A) Liver p66Shc mRNA expression, n=6. (B) Liver p66Shc, SOD2, UCP1, Col1a1, α-SMA protein, n=3. (C) H2O2 content, n=8. (D) SOD activity, n=8. (E) Cytochrome c expression in the cytoplasm and mitochondria, n=3. (F) H&E and Masson staining. Scale bar, 200 μm. (G) Ishak score of Masson staining. (H) Serum ALT and AST levels, n=8. (I) Liver NLRP3 inflammasome protein expression, n=3.
3). Inflammation-driven biomimetic nano-polyphenol drug delivery system alleviates severe acute pancreatitis by inhibiting macrophage PANoptosis and pancreatic enzymes oversecretion. Journal of advanced research, 2025 (PubMed: 40210149) [IF=11.4]
4). Water-extracted Lonicera japonica polysaccharide attenuates allergic rhinitis by regulating NLRP3-IL-17 signaling axis. Carbohydrate Polymers, 2022 (PubMed: 36184153) [IF=10.7]
5). AIM2 Inflammasome Mediates Hallmark Neuropathological Alterations and Cognitive Impairment in a Mouse Model of Vascular Dementia. Molecular Psychiatry, 2020 (PubMed: 33299135) [IF=9.6]
6). NLRP3-GABA signaling pathway contributes to the pathogenesis of impulsive-like behaviors and cognitive deficits in aged mice. Journal of neuroinflammation, 2023 (PubMed: 37434240) [IF=9.3]

Application: IF/ICC    Species: Mouse    Sample: hippocampus

Fig. 6 Cognitive impairment may be related to pyroptosis-associated inflammatory responses in astrocytes. A Representative photomicrographs of NLRP3-, IL-18-, IL-1β- and cleaved caspase-1-positive cells in the CA1 of the hippocampus at 12 h and 10 days after surgical exposure. Scale bar = 50 μm. B Image of CA1 in hippocampus. C–J Co-stained area of NLRP3-, IL-18-, IL-1β-, cleaved caspase-1- and GFAP-positive cells in the CA1 of the hippocampus. K Representative western blot of NLRP3, IL-1β, IL-18 and cleaved caspase-1 at 12 h after surgical exposure. L–O The optical density values of NLRP3, IL-1β, IL-18, and cleaved caspase-1 at 12 h after surgical exposure. P Representative western blot of NLRP3, IL-1β, IL-18 and cleaved caspase-1 at 10 days after surgical exposure. Q–T The optical density values of NLRP3, IL-1β, IL-18 and cleaved caspase-1 at 10 days after surgical exposure. Data are presented as the mean ± SD (n = 6 mice/group or n = 3 mice/group). Data were analyzed by one-way ANOVA with Tukey’s multiple comparison test or Kruskal–Wallis and Dunn’s multiple comparison test. ***P 
7). Mesenchymal stem cells protect against TBI-induced pyroptosis in vivo and in vitro through TSG-6. Cell Communication and Signaling, 2022 (PubMed: 35982465) [IF=8.4]
8). Physicochemical characterization of polysaccharide from the leaf of Dendrobium officinale and effect on LPS induced damage in GES-1 cell. International Journal of Biological Macromolecules, 2020 (PubMed: 31945440) [IF=7.7]

Application: WB    Species: Human    Sample: GES-1 cells

Fig. 9. LDOP-1 protects LPS-induced GES-1 damage through inhibiting the TLR4/NF-kb signaling pathway. (A) The protein expressions of cleaved-IL-1β, IL-6, pro-caspase 1 Caspase 1, NF- κB, phospho-NF-κB, TLR4, NLRP3, ASC, phospho-IκBα and IκBα, (B–D) Expression levels of Cleaved-IL-1β, IL-6 and ASC protein were analyzed. (E–G) Protein expression levels ratio of cleaved-caspase-1 to pro-caspase-1, phospho-IκBα to IκBα and, phospho-NF-кB to NF-κB. (H–I) Expression levels of NLRP3 and TLR4 protein were analyzed. The location assay of by laser scanning confocal microscopy. β-Actin was used as control. (J) Expression of IL-6 were observed by fluorescence microscopy, red indicated positive immunofluorescence results for IL-6, blue indicated the result of DAPI. The data were presented as the mean ± SD from three independent experiments (n = 3). Compared to the control group, #p b 0.05, ##p b 0.01; compared to the model group *p b 0.05, **p b 0.01.
9). Recombinant Antithrombin Alleviated Pulmonary Injury and Inflammation in LPS-Induced ARDS by Inhibiting IL17a/NF-κB Signaling. ImmunoTargets and therapy, 2025 (PubMed: 40226836) [IF=7.2]
10). Disulfiram alleviates pristane-induced lupus via inhibiting GSDMD-mediated pyroptosis. Cell Death Discovery, 2022 (PubMed: 36057687) [IF=7.0]

Application: IF/ICC    Species: Human    Sample: THP-1 cells

Fig. 2 GSDMD-mediated pyroptosis of THP-1 cells induced by serum from SLE patients was suppressed by DSF. A Effect of DSF on the cell viability of THP-1 cells. Cells were treated with DSF (0, 1, 5, 10, 20 μM) for 48 h and then cell viability measured by CCK-8 assay. B The morphological features of THP-1 cells treated with serums from healthy controls or SLE patients, with or without mixing 10 μM disulfiram. C Lactate dehydrogenase (LDH) release from THP-1 cells treated as indicated. D Hoechst33342/Propidium Iodide (PI) double staining in THP-1 cells after different treatments. E Representative immunofluorescence images showing the expression of NLRP3, caspase-1, and cleaved caspase-1 in THP-1 cells treated as indicated. F The expression of full length and cleaved GSDMD in THP-1 cells. The cells were incubated in different mediums and analyzed by western blot analysis. β-actin was used as a protein loading control. G, H The expression level of total GSDMD and cleaved-GSDMD relative to β-actin were quantified. Total GSDMD = full length-GSDMD + cleaved-GSDMD. Significant differences were calculated using one-way ANOVA. Values were shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001. Each experiment was repeated three times. FBS fetal bovine serum, HC Healthy control, NS not significant, PI propidium iodide, LDH Lactate dehydrogenase.
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