Phospho-FAK (Tyr397) Antibody - #AF3398

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产品: 磷酸化 FAK (Tyr397) 抗体
货号: AF3398
描述: Rabbit polyclonal antibody to Phospho-FAK (Tyr397)
应用: WB IHC IF/ICC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q05397
RRID: AB_2834829

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:100-1:500, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-FAK (Tyr397) Antibody detects endogenous levels of FAK only when phosphorylated at Tyrosine 397.
RRID:
AB_2834829
引用格式: Affinity Biosciences Cat# AF3398, RRID:AB_2834829.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FADK 1; FADK; FAK related non kinase polypeptide; FAK1; FAK1_HUMAN; Focal adhesion kinase 1; Focal adhesion Kinase; Focal adhesion kinase isoform FAK Del33; Focal adhesion kinase related nonkinase; FRNK; p125FAK; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; Protein tyrosine kinase 2; Protein-tyrosine kinase 2; Ptk2; PTK2 protein tyrosine kinase 2;

抗原和靶标

免疫原:

A synthesized peptide derived from human FAK around the phosphorylation site of Tyr397.

基因/基因ID:
PTK2(5747)
描述:
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). Biomimetic MOF nanoplatform for dual-targeted co-delivery of FAK inhibitor and bismuth to enhance cervical cancer radiosensitivity. Advanced Composites and Hybrid Materials, 2025 [IF=23.2]

Application: WB    Species: Mouse    Sample:

Fig. 2 IN10018 reduced CAF infiltration in tumors, thereby enhancing radiosensitivity of cervical cancer. A Representative images of IHC of Ki67, α-SMA, Iba1, and CD31 in tumors. The bar chart means quantitative analyses of IHC. Data represent mean ± SD of each group (n = 5); *P 

Application: IHC    Species: Mouse    Sample:

Fig. 9 IZB@CCM suppressed CAF infiltration in tumors, thus further improving the radiosensitivity of cervical cancer. A Representative images of IHC of p-FAK, α-SMA and Ki67 in tumors. B The bar chart means quantitative analyses of IHC. Data represent mean ± SD of each group (n = 5); *P 
2). Nanocarrier Nanomechanics: A Key to Unlocking Endocytosis in Cancer Cells Navigating Epithelial-Mesenchymal Transition Dynamics. ACS Nano, 2025 [IF=16.0]
3). NELL2, a novel osteoinductive factor, regulates osteoblast differentiation and bone homeostasis through fibronectin 1/integrin-mediated FAK/AKT signaling. Bone Research, 2025 [IF=15.0]
4). A tropomyosin-like Meretrix meretrix Linnaeus polypeptide inhibits the proliferation and metastasis of glioma cells via microtubule polymerization and FAK/Akt/MMPs signaling. International Journal of Biological Macromolecules, 2020 (PubMed: 31866539) [IF=7.7]

Application: WB    Species: Human    Sample: U87 cells

Fig. 5. re-MM15 inhibited the metastasis of U87 cells through downregulating FAK/Akt/MMPs signaling. (A and B) Suppression of U87 cell migration by re-MM15. Wound closures of U87 cells were photographed at 0 h, 12 h and 24 h following treatment with different concentrations of re-MM15 (scale bar: 100 μm). Error bars represented the mean ± SEM of triplicate independent experiments. (C–D) Inhibition of U87 cell invasion by re-MM15. After treated with indicated concentrations of re-MM15, U87 cells were seeded in Matrigel-coated transwell chambers and the invaded cells were stained and calculated. Shown are representative images of invaded cells (scale bar: 100 μm). The bar graphs showed mean ± SEM of the numbers of invaded cells from three independent assays. (E–F) Downregulation of FAK/Akt/MMPs signaling by re-MM15. The expression of p-FAK, FAK, p-Akt, Akt, MMP-2, MMP- 9 were detected by Western blot. Band density was quantified and normalized with GAPDH. The results shown are mean ± SEM (n = 3). *P b 0.05, **P b 0.01 versus control group.
5). Catalpol ameliorates liver fibrosis via inhibiting aerobic glycolysis by EphA2/FAK/Src signaling pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 39321687) [IF=6.7]
6). Anti-proliferation and anti-migration effects of Yishen Tongbi decoction in experimental rheumatoid arthritis by suppressing SLC3A2/integrin β3 signaling pathways. Phytomedicine, 2023 (PubMed: 36990010) [IF=6.7]
7). Baicalin reduces chronic stress-induced breast cancer metastasis via directly targeting β2-adrenergic receptor. Journal of pharmaceutical analysis, 2024 (PubMed: 39139999) [IF=6.1]
8). Down-regulating Myoferlin inhibits the vasculogenic mimicry of melanoma via decreasing MMP-2 and inducing mesenchymal-to-epithelial transition. Journal of Cellular and Molecular Medicine, 2018 (PubMed: 29164766) [IF=5.3]
9). Phellinus linteus activates Treg cells via FAK to promote M2 macrophage polarization in hepatocellular carcinoma. Cancer immunology, immunotherapy : CII, 2024 (PubMed: 38240856) [IF=4.6]

Application: WB    Species: Mouse    Sample:

Fig. 3 FAK promotes the polarization of macrophages in the Treg-macrophage co-culture system through PI3K/AKT/JAK/STAT3 and p38/JNK/ERK signal pathway A, B The expression of the PI3K, p-PI3K, AKT, p-AKT, p-FAK, FAK, JAK2, STAT3, p-STAT3 and p38, p-p38/JNK, p-JNK/ERK1/2, p-ERK1/2 in Treg-macrophage co-culture system were observed by the western blot, n = 3 in each group. #P 
10). Focal adhesion kinase-related non-kinase ameliorates liver fibrosis by inhibiting aerobic glycolysis via the FAK/Ras/c-myc/ENO1 pathway. WORLD JOURNAL OF GASTROENTEROLOGY, 2022 (PubMed: 35125823) [IF=4.3]

Application: IHC    Species: Human    Sample: fibrotic liver tissue

Figure 1 Tyrosine phosphorylation at position 397 of FAK is upregulated, while FRNK expression is downregulated in human fibrotic liver tissue. A: H&E, Masson’s trichrome and Sirius Red staining were performed after liver biopsy to assess the tissues of normal subjects and patients with liver fibrosis under a light microscope at 200× magnification; B: Immunohistochemistry showed changes in the expression of α-smooth muscle actin (α-SMA) and tyrosine phosphorylation at position 397 of FAK(pY397-FAK) in the livers of normal subjects compared with patients with liver fibrosis under a light microscope at 200× or 400× magnification; C and D: Protein expression in biopsy tissues was analyzed using Western blotting. Representative results from three independent replicate assays are shown. aP < 0.05. Data are presented as the mean ± SD. FAK: Focal adhesion kinase; FRNK: Focal adhesion kinase-related non-kinase; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.

Application: WB    Species: Human    Sample: fibrotic liver tissue

Figure 1 Tyrosine phosphorylation at position 397 of FAK is upregulated, while FRNK expression is downregulated in human fibrotic liver tissue. A: H&E, Masson’s trichrome and Sirius Red staining were performed after liver biopsy to assess the tissues of normal subjects and patients with liver fibrosis under a light microscope at 200× magnification; B: Immunohistochemistry showed changes in the expression of α-smooth muscle actin (α-SMA) and tyrosine phosphorylation at position 397 of FAK(pY397-FAK) in the livers of normal subjects compared with patients with liver fibrosis under a light microscope at 200× or 400× magnification; C and D: Protein expression in biopsy tissues was analyzed using Western blotting. Representative results from three independent replicate assays are shown. aP < 0.05. Data are presented as the mean ± SD. FAK: Focal adhesion kinase; FRNK: Focal adhesion kinase-related non-kinase; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.
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