产品: 磷酸化 FAK (Tyr576) 抗体
货号: AF3397
描述: Rabbit polyclonal antibody to Phospho-FAK (Tyr576)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
蛋白号: Q05397
RRID: AB_2834828

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 50ul RMB¥ 1300 现货
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 200ul RMB¥ 3200 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
Phospho-FAK (Tyr576) Antibody detects endogenous levels of FAK only when phosphorylated at Tyrosine 576.
RRID:
AB_2834828
引用格式: Affinity Biosciences Cat# AF3397, RRID:AB_2834828.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FADK 1; FADK; FAK related non kinase polypeptide; FAK1; FAK1_HUMAN; Focal adhesion kinase 1; Focal adhesion Kinase; Focal adhesion kinase isoform FAK Del33; Focal adhesion kinase related nonkinase; FRNK; p125FAK; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; Protein tyrosine kinase 2; Protein-tyrosine kinase 2; Ptk2; PTK2 protein tyrosine kinase 2;

抗原和靶标

免疫原:

A synthesized peptide derived from human FAK around the phosphorylation site of Tyr576.

基因/基因ID:
描述:
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Small cell lung cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

文献引用

1). NSun2-Mediated tsRNAs Alleviate Liver Fibrosis via FAK Dephosphorylation. Cell proliferation, 2025 (PubMed: 40355098) [IF=5.9]

2). Danggui Buxue Tang promotes the adhesion and migration of bone marrow stromal cells via the focal adhesion pathway in vitro. JOURNAL OF ETHNOPHARMACOLOGY, 2019 (PubMed: 30445110) [IF=4.8]

Application: WB    Species: mouse    Sample: BMSCs

Figure 8 |Western blot analysis of key proteins in the focal adhesion pathway after DBT treatment for 6 days. (A, C) Representatives blot from 3 independent experiments. (B, D) Densitometric quantification. GAPDH was used as an internal control. Values are the mean ± SEM, where n = 3. (*p < 0.05 as compared to the control, LSD).

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