Phospho-WIPI2 (Ser413) Antibody - #AF2434

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产品: 磷酸化 WIPI2 (Ser413) 抗体
货号: AF2434
描述: Rabbit polyclonal antibody to Phospho-WIPI2 (Ser413)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Sheep, Rabbit, Dog, Chicken
分子量: 49kDa; 49kD(Calculated).
蛋白号: Q9Y4P8
RRID: AB_2845447

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(90%), Sheep(82%), Rabbit(91%), Dog(92%), Chicken(92%)
克隆:
Polyclonal
特异性:
Phospho-WIPI2 (Ser413) Antibody detects endogenous levels of WIPI2 only when phosphorylated at Ser413.
RRID:
AB_2845447
引用格式: Affinity Biosciences Cat# AF2434, RRID:AB_2845447.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ATG18B; Atg21; CGI 50; DKFZp434J154; DKFZp686P02188; FLJ12979; FLJ14217; FLJ42984; WD repeat domain phosphoinositide-interacting protein 2; WD repeat domain, phosphoinositide interacting 2; WD40 repeat protein interacting with phosphoinositides 2; WIPI 2; WIPI-2; WIPI2; WIPI2_HUMAN; WIPI49 like protein 2; WIPI49-like protein 2;

抗原和靶标

免疫原:
Uniprot:

Q9Y4P8(WIPI2_HUMAN) >>Visit HPA database.

基因/基因ID:
WIPI2(26100)
表达:
Q9Y4P8 WIPI2_HUMAN:

Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.

序列:
MNLASQSGEAGAGQLLFANFNQDNTEVKGASRAAGLGRRAVVWSLAVGSKSGYKFFSLSSVDKLEQIYECTDTEDVCIVERLFSSSLVAIVSLKAPRKLKVCHFKKGTEICNYSYSNTILAVKLNRQRLIVCLEESLYIHNIRDMKVLHTIRETPPNPAGLCALSINNDNCYLAYPGSATIGEVQVFDTINLRAANMIPAHDSPLAALAFDASGTKLATASEKGTVIRVFSIPEGQKLFEFRRGVKRCVSICSLAFSMDGMFLSASSNTETVHIFKLETVKEKPPEEPTTWTGYFGKVLMASTSYLPSQVTEMFNQGRAFATVRLPFCGHKNICSLATIQKIPRLLVGAADGYLYMYNLDPQEGGECALMKQHRLDGSLETTNEILDSASHDCPLVTQTYGAAAGKGTYVPSSPTRLAYTDDLGAVGGACLEDEASALRLDEDSEHPPMILRTD

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Dog
92
Chicken
92
Rabbit
91
Pig
90
Sheep
82
Xenopus
75
Bovine
73
Horse
70
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9Y4P8 作为底物

Site PTM Type Enzyme
K28 Ubiquitination
K146 Ubiquitination
K216 Ubiquitination
K223 Acetylation
K223 Ubiquitination
K237 Ubiquitination
K283 Ubiquitination
T292 Phosphorylation
S308 Phosphorylation
K331 Ubiquitination
K341 Ubiquitination
K371 Ubiquitination
S378 Phosphorylation
S412 Phosphorylation
S413 Phosphorylation
T415 Phosphorylation

研究背景

功能:

Component of the autophagy machinery that controls the major intracellular degradation process by which cytoplasmic materials are packaged into autophagosomes and delivered to lysosomes for degradation. Involved in an early step of the formation of preautophagosomal structures. Binds and is activated by phosphatidylinositol 3-phosphate (PtdIns3P) forming on membranes of the endoplasmic reticulum upon activation of the upstream ULK1 and PI3 kinases. Once activated, WIPI2 recruits at phagophore assembly sites the ATG12-ATG5-ATG16L1 complex that directly controls the elongation of the nascent autophagosomal membrane.

Recruits the ATG12-ATG5-ATG16L1 complex to omegasomes and preautophagosomal structures, resulting in ATG8 family proteins lipidation and starvation-induced autophagy. Isoform 4 is also required for autophagic clearance of pathogenic bacteria. Isoform 4 binds the membrane surrounding Salmonella and recruits the ATG12-5-16L1 complex, initiating LC3 conjugation, autophagosomal membrane formation, and engulfment of Salmonella.

细胞定位:

Preautophagosomal structure membrane>Peripheral membrane protein>Cytoplasmic side.
Note: Localizes to omegasomes membranes which are endoplasmic reticulum connected structures at the origin of preautophagosomal structures. Enriched at preautophagosomal structure membranes in response to PtdIns3P.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed (at protein level). Highly expressed in heart, skeletal muscle and pancreas. Expression is down-regulated in pancreatic and in kidney tumors.

亚基结构:

Interacts with TECPR1. Interacts with ATG16L1. Interacts with ATG5. Interacts with WIPI1. Interacts with WDR45. May interact with NUDC.

蛋白家族:

The FRRG motif is required for recruitment to PtdIns3P.

Belongs to the WD repeat SVP1 family.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - other.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

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