Phospho-TNK1 (Tyr277) Antibody - #AF2426

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产品: 磷酸化 TNK1 (Tyr277) 抗体
货号: AF2426
描述: Rabbit polyclonal antibody to Phospho-TNK1 (Tyr277)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Rabbit, Dog
分子量: 72kDa; 72kD(Calculated).
蛋白号: Q13470
RRID: AB_2845439

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 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(92%), Horse(100%), Rabbit(92%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-TNK1 (Tyr277) Antibody detects endogenous levels of TNK1 only when phosphorylated at Tyr277.
RRID:
AB_2845439
引用格式: Affinity Biosciences Cat# AF2426, RRID:AB_2845439.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CD38 negative kinase 1; EC 2.7.10.2; Kinase of embryonic stem cells; Kos 1; Kos1; MGC46193; Non receptor tyrosine protein kinase TNK 1; Non receptor tyrosine protein kinase TNK1; Non-receptor tyrosine-protein kinase TNK1; Tnk 1; Tnk1; TNK1_HUMAN; Tyrosine kinase non receptor 1;

抗原和靶标

免疫原:
Uniprot:

Q13470(TNK1_HUMAN) >>Visit HPA database.

基因/基因ID:
TNK1(8711)
表达:
Q13470 TNK1_HUMAN:

Expressed in all umbilical cord blood, bone marrow and adult blood cell sub-populations and in several leukemia cell lines. Highly expressed in fetal blood, brain, lung, liver and kidney. Detected at lower levels in adult prostate, testis, ovary, small intestine and colon. Not expressed in adult lung, liver, kidney or brain.

序列:
MLPEAGSLWLLKLLRDIQLAQFYWPILEELNVTRPEHFDFVKPEDLDGIGMGRPAQRRLSEALKRLRSGPKSKNWVYKILGGFAPEHKEPTLPSDSPRHLPEPEGGLKCLIPEGAVCRGELLGSGCFGVVHRGLWTLPSGKSVPVAVKSLRVGPEGPMGTELGDFLREVSVMMNLEHPHVLRLHGLVLGQPLQMVMELAPLGSLHARLTAPAPTPPLLVALLCLFLRQLAGAMAYLGARGLVHRDLATRNLLLASPRTIKVADFGLVRPLGGARGRYVMGGPRPIPYAWCAPESLRHGAFSSASDVWMFGVTLWEMFSGGEEPWAGVPPYLILQRLEDRARLPRPPLCSRALYSLALRCWAPHPADRPSFSHLEGLLQEAGPSEACCVRDVTEPGALRMETGDPITVIEGSSSFHSPDSTIWKGQNGRTFKVGSFPASAVTLADAGGLPATRPVHRGTPARGDQHPGSIDGDRKKANLWDAPPARGQRRNMPLERMKGISRSLESVLSLGPRPTGGGSSPPEIRQARAVPQGPPGLPPRPPLSSSSPQPSQPSRERLPWPKRKPPHNHPMGMPGARKAAALSGGLLSDPELQRKIMEVELSVHGVTHQECQTALGATGGDVVSAIRNLKVDQLFHLSSRSRADCWRILEHYQWDLSAASRYVLARP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Dog
100
Bovine
92
Rabbit
92
Zebrafish
67
Sheep
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13470 作为底物

Site PTM Type Enzyme
S60 Phosphorylation
S68 Phosphorylation
K73 Ubiquitination
Y77 Phosphorylation
K88 Ubiquitination
S94 Phosphorylation
S96 Phosphorylation
K108 Ubiquitination
S139 Phosphorylation
K141 Ubiquitination
T209 Phosphorylation
T214 Phosphorylation
Y235 Phosphorylation
T248 Phosphorylation
S255 Phosphorylation
T258 Phosphorylation
K260 Ubiquitination
Y277 Phosphorylation
Y287 Phosphorylation
Y353 Phosphorylation
S354 Phosphorylation
S468 Phosphorylation
K475 Methylation
S500 Phosphorylation
S502 Phosphorylation O14757 (CHEK1)
S505 Phosphorylation
S508 Phosphorylation
T514 Phosphorylation
S518 Phosphorylation
S519 Phosphorylation
S546 Phosphorylation
S550 Phosphorylation
S553 Phosphorylation
K563 Ubiquitination
K577 Ubiquitination
S582 Phosphorylation
S587 Phosphorylation
T612 Phosphorylation
Y651 Phosphorylation
S659 Phosphorylation
Y661 Phosphorylation

研究背景

功能:

Involved in negative regulation of cell growth. Has tumor suppressor properties. Plays a negative regulatory role in the Ras-MAPK pathway. May function in signaling pathways utilized broadly during fetal development and more selectively in adult tissues and in cells of the lymphohematopoietic system. Could specifically be involved in phospholipid signal transduction.

翻译修饰:

Autophosphorylated on tyrosine residues.

细胞定位:

Cytoplasm. Membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in all umbilical cord blood, bone marrow and adult blood cell sub-populations and in several leukemia cell lines. Highly expressed in fetal blood, brain, lung, liver and kidney. Detected at lower levels in adult prostate, testis, ovary, small intestine and colon. Not expressed in adult lung, liver, kidney or brain.

亚基结构:

Interacts with the SH3 domain of PLCG1 via its Pro-rich domain.

蛋白家族:

Belongs to the protein kinase superfamily. Tyr protein kinase family.

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