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![Phospho-Tau (Ser199)[Ser516] Antibody - Western blot analysis of extracts from 3T3-L1 cells(heat-shock treatment)HepG2 cells(heat-shock treatment) Hela cells(heat-shock treatment), using Phospho-Tau (Ser199)[Ser516] Antibody.](http://img.affbiotech.cn/images/202207/af2418_59935_phospho_tau_ser199_ser516_antibody_1658977559.jpg "Western blot analysis of extracts from 3T3-L1 cells(heat-shock treatment)HepG2 cells(heat-shock treatment) Hela cells(heat-shock treatment), using Phospho-Tau (Ser199)[Ser516] Antibody. The lane on the left was treated with blocking peptide.")
![Phospho-Tau (Ser199)[Ser516] Antibody - AF2418 at 1/100 staining Rat brain tissue by IHC-P.](http://img.affbiotech.cn/images/202004/thumb_img/af2418_phospho_tau_ser199_ser516_antibody_thumb_P_1586770836797.jpg "AF2418 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.")
![Phospho-Tau (Ser199)[Ser516] Antibody - peptide-ELISA analysis of AF2418.](http://img.affbiotech.cn/images/pelisa/809/af2418-peptide-elisa.png "peptide-ELISA analysis of AF2418. showing specificity to antigen peptide. Peptides concentration: 1ug/ml.<br>
P-peptide: phospho-peptide. N-peptide: non-phospho-peptide.")
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2418, RRID:AB_2845432.
展开/折叠
AI413597; AW045860; DDPAC; FLJ31424; FTDP 17; G protein beta1/gamma2 subunit interacting factor 1; MAPT; MAPTL; MGC134287; MGC138549; MGC156663; Microtubule associated protein tau; Microtubule associated protein tau isoform 4; Microtubule-associated protein tau; MSTD; Mtapt; MTBT1; MTBT2; Neurofibrillary tangle protein; Paired helical filament tau; Paired helical filament-tau; PHF tau; PHF-tau; PPND; PPP1R103; Protein phosphatase 1, regulatory subunit 103; pTau; RNPTAU; TAU; TAU_HUMAN; Tauopathy and respiratory failure, included;
抗原和靶标
A synthesized peptide derived from human Tau around the phosphorylation site of Ser199.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.
文献引用
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