![Phospho-SHIP1 (Tyr1020)[Tyr1022] Antibody - Western blot analysis of extracts from UV treated MCF7 cells, using Phospho-SHIP1 (Tyr1020) Antibody.](http://img.affbiotech.cn/images/201908/thumb_img/af2403_phospho_ship1_tyr1020_antibody_thumb_P_1567145666430.jpg "Western blot analysis of extracts from UV treated MCF7 cells, using Phospho-SHIP1 (Tyr1020) Antibody. The lane on the left was treated with blocking peptide.")
![Phospho-SHIP1 (Tyr1020)[Tyr1022] Antibody - Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using Phospho-SHIP1 (Tyr1020)[Tyr1022] Antibody at 1/1000 dilution.](http://img.affbiotech.cn/images/202307/af2403_63720_phospho_ship1_tyr1020_tyr1022_antibody_1689321119.jpg "Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using Phospho-SHIP1 (Tyr1020)[Tyr1022] Antibody at 1/1000 dilution.
Observed bands:160kD.")
![Phospho-SHIP1 (Tyr1020)[Tyr1022] Antibody - Western blot analysis of extracts from EC304 cells(heat-shock treatment), using Phospho-SHIP1 (Tyr1020)[Tyr1022] Antibody at 1/1000 dilution.](http://img.affbiotech.cn/images/202307/af2403_63721_phospho_ship1_tyr1020_tyr1022_antibody_1689321119.jpg "Western blot analysis of extracts from EC304 cells(heat-shock treatment), using Phospho-SHIP1 (Tyr1020)[Tyr1022] Antibody at 1/1000 dilution.
Observed bands:150kD.")
![Phospho-SHIP1 (Tyr1020)[Tyr1022] Antibody - AF2403 staining heat-shock treated Hela cells by IF/ICC.](http://img.affbiotech.cn/images/201912/thumb_img/af2403_phospho_ship1_tyr1020_tyr1022_antibody_thumb_P_1575345367568.jpg "AF2403 staining heat-shock treated Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF2403) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.
The nuclear counter stain is DAPI(blue).")
![Phospho-SHIP1 (Tyr1020)[Tyr1022] Antibody - peptide-ELISA analysis of AF2403.](http://img.affbiotech.cn/images/pelisa/809/af2403-peptide-elisa.png "peptide-ELISA analysis of AF2403. showing specificity to antigen peptide. Peptides concentration: 1ug/ml.<br>
P-peptide: phospho-peptide. N-peptide: non-phospho-peptide.")
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2403, RRID:AB_2845417.
展开/折叠
Inositol polyphosphate 5 phosphatase of 145kDa; 4; 5-trisphosphate 5-phosphatase 1; hp51CN; Inositol polyphosphate 5 phosphatase 145kDa; Inositol polyphosphate 5 phosphatase; Inositol polyphosphate-5-phosphatase of 145 kDa; INPP5D; MGC104855; MGC142140; MGC142142; p150Ship; Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1; Phosphatidylinositol-3; SH2 containing inositol phosphatase isoform b; SH2 domain containing inositol 5' phosphatase 1; SH2 domain containing inositol phosphatase 1; SH2 domain-containing inositol phosphatase 1; SH2 domain-containing inositol-5''-phosphatase 1; SHIP-1; SHIP1; SHIP1_HUMAN; Signaling inositol polyphosphate 5 phosphatase SIP 145; SIP-145; SIP145;
抗原和靶标
A synthesized peptide derived from human SHIP1 around the phosphorylation site of Tyr1020.
研究领域
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc epsilon RI signaling pathway. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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