Phospho-MST1(Thr183)/MST2(Thr180) Antibody - #AF2367

Figure 7 PRMT5 and MST2‐SDMA are elevated but pMST2‐Thr180 is reduced in PDAC, and GSK3326595 suppresses the progression of pancreatic cancer A, B The representative IHC images of PRMT5, α‐me2s and pMST2‐Thr180 proteins on TMA (n = 37) of pancreatic cancer specimens were shown (A). Scale bars were indicated in the figure. The heatmap showed IHC score of PRMT5, α‐me2s and pMST2‐Thr180 proteins on TMA (n = 37) of pancreatic cancer specimens (B). C Twelve pairs of pancreatic cancer tissues and their matched para‐carcinoma tissues were extracted proteins. MST2 was immunopurified to compare the level of α‐me2s and phospho‐Thr180 between cancer tissues and para‐carcinoma tissues. Relative ratio was quantified and normalized to MST2. The level of PRMT5 and total α‐me2s was also compared. Immunoblotting was repeated technically three times independently. N = 12 biological replicates. D Relative α‐me2s level of MST2, relative pMST2‐Thr180 level of MST2, relative PRMT5 level, and relative total α‐me2s level of pancreatic cancer tissues and para‐carcinoma tissues were quantified and shown as the violin plots. The statistical significance was tested by the paired t‐test. Immunoblotting was repeated technically three times independently. N = 12 biological replicates. **P 

Figure 7 PRMT5 and MST2‐SDMA are elevated but pMST2‐Thr180 is reduced in PDAC, and GSK3326595 suppresses the progression of pancreatic cancer A, B The representative IHC images of PRMT5, α‐me2s and pMST2‐Thr180 proteins on TMA (n = 37) of pancreatic cancer specimens were shown (A). Scale bars were indicated in the figure. The heatmap showed IHC score of PRMT5, α‐me2s and pMST2‐Thr180 proteins on TMA (n = 37) of pancreatic cancer specimens (B). C Twelve pairs of pancreatic cancer tissues and their matched para‐carcinoma tissues were extracted proteins. MST2 was immunopurified to compare the level of α‐me2s and phospho‐Thr180 between cancer tissues and para‐carcinoma tissues. Relative ratio was quantified and normalized to MST2. The level of PRMT5 and total α‐me2s was also compared. Immunoblotting was repeated technically three times independently. N = 12 biological replicates. D Relative α‐me2s level of MST2, relative pMST2‐Thr180 level of MST2, relative PRMT5 level, and relative total α‐me2s level of pancreatic cancer tissues and para‐carcinoma tissues were quantified and shown as the violin plots. The statistical significance was tested by the paired t‐test. Immunoblotting was repeated technically three times independently. N = 12 biological replicates. **P 

Fig. 6.| PD inhibits liver fibrosis through regulation of Hippo pathway. (A) LX-2 cells were treated with PD (5 μM, 10 μM, 20 μM) for 24 h with or without TGF-β1(5 ng/ml). Western blot analysis of Hippo signaling core factor protein expression.

Figure 6. |Res Reduces ST6GAL2 Expression and Activates the Hippo Signaling Pathway in FTC Cells(A–D) The qPCR and western blotting results indicated that ST6GAL2 expression changes after Res treatment in FTC cells. (E–G) Expression of the main protein components of the Hippo signaling pathway in FTC238 cells was assessed by western blotting. Also shown is western blot analysis of nuclear YAP and TAZ expression in the indicated cells. The nuclear protein histone H3 was used as the nuclear protein marker