Phospho-Atg14 (Ser29) Antibody - #AF2320

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产品: 磷酸化 Atg14 (Ser29) 抗体
货号: AF2320
描述: Rabbit polyclonal antibody to Phospho-Atg14 (Ser29)
应用: WB IHC
文献验证: WB
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
蛋白号: Q6ZNE5
RRID: AB_2845334

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse
克隆:
Polyclonal
特异性:
Phospho-Atg14 (Ser29) Antibody detects endogenous levels of Atg14 only when phosphorylated at Ser29.
RRID:
AB_2845334
引用格式: Affinity Biosciences Cat# AF2320, RRID:AB_2845334.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

4832427M01; ATG14; Atg14L; Autophagy-related protein 14-like protein; BAKOR_HUMAN; Barkor; Beclin 1-associated autophagy-related key regulator; D14Ertd114e; D14Ertd436e; KIAA0831; mCG_6911;

抗原和靶标

免疫原:

A synthesized peptide derived from human Atg14 around the phosphorylation site of Ser29.

基因/基因ID:
ATG14(22863)

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

文献引用

1). Dihydroartemisinin Ameliorates Learning and Memory in Alzheimer’s Disease Through Promoting Autophagosome-Lysosome Fusion and Autolysosomal Degradation for Aβ Clearance. Frontiers in Aging Neuroscience, 2020 (PubMed: 32210783) [IF=4.1]

Application: WB    Species: Mouse    Sample: brain tissue

Figure 6 DHA increases the basal level of autophagy. Protein levels of ATG5, ATG12, ATG16L1, Beclin1, ATG14, p-ATG14 (Ser29), Rab7, RILP, Lamp1, CTSB, p-mTOR(Ser2448), mammalian target of rapamycin (mTOR), ULK1, p-GSK3β (Ser9), GSK3β in extracts of whole brain tissue were detected by WB. Representative Western blots are presented in panels (A,C,E,G), and quantification of the results is shown as the mean values ± SD in panels (B,D,F,H). *p < 0.05, **p < 0.01, and ***p < 0.001 between two groups. One-way analysis of variance/Newman–Keuls test was performed for all WB data.

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