产品: 磷酸化 MEF2A (Thr312) 抗体
货号: AF3381
描述: Rabbit polyclonal antibody to Phospho-MEF2A (Thr312)
应用: WB IHC IF/ICC IP
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 55kDa; 55kD(Calculated).
蛋白号: Q02078
RRID: AB_2834812

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IP, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(82%)
克隆:
Polyclonal
特异性:
Phospho-MEF2A (Thr312) Antibody detects endogenous levels of MEF2A only when phosphorylated at Threonine 312.
RRID:
AB_2834812
引用格式: Affinity Biosciences Cat# AF3381, RRID:AB_2834812.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ADCAD1; MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A); MEF2; MEF2A; MEF2A_HUMAN; Myocyte enhancer factor 2A; Myocyte-specific enhancer factor 2A; RSRFC4; RSRFC9; Serum response factor like protein 1; Serum response factor-like protein 1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q02078 MEF2A_HUMAN:

Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.

描述:
MEF2A a myocyte-specific enhancing transcription factor which binds specifically to the MEF2 element present in the regulatory regions of many, if not all, muscle-specific genes. A member of the MADS gene family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain.
序列:
MGRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSSNKLFQYASTDMDKVLLKYTEYNEPHESRTNSDIVEALNKKEHRGCDSPDPDTSYVLTPHTEEKYKKINEEFDNMMRNHKIAPGLPPQNFSMSVTVPVTSPNALSYTNPGSSLVSPSLAASSTLTDSSMLSPPQTTLHRNVSPGAPQRPPSTGNAGGMLSTTDLTVPNGAGSSPVGNGFVNSRASPNLIGATGANSLGKVMPTKSPPPPGGGNLGMNSRKPDLRVVIPPSSKGMMPPLSEEEELELNTQRISSSQATQPLATPVVSVTTPSLPPQGLVYSAMPTAYNTDYSLTSADLSALQGFNSPGMLSLGQVSAWQQHHLGQAALSSLVAGGQLSQGSNLSINTNQNISIKSEPISPPRDRMTPSGFQQQQQQQQQQQPPPPPQPQPQPPQPQPRQEMGRSPVDSLSSSSSSYDGSDREDPRGDFHSPIVLGRPPNTEDRESPSVKRMRMDAWVT

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
91
Chicken
91
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q02078 作为底物

Site PTM Type Enzyme
T20 Phosphorylation
K23 Ubiquitination
S59 Phosphorylation
K64 Ubiquitination
K68 Ubiquitination
S98 Phosphorylation Q16539 (MAPK14)
T103 Phosphorylation
S104 Phosphorylation
Y105 Phosphorylation
T108 Phosphorylation Q16539 (MAPK14)
T111 Phosphorylation
Y115 Phosphorylation
S192 Phosphorylation Q16539 (MAPK14)
S223 Phosphorylation Q16539 (MAPK14)
S235 Phosphorylation
S246 Phosphorylation
K249 Acetylation
T253 Phosphorylation
S255 Phosphorylation
S289 Phosphorylation P68400 (CSNK2A1)
T312 Phosphorylation Q16539 (MAPK14) , Q13164 (MAPK7)
T319 Phosphorylation Q13164 (MAPK7) , Q16539 (MAPK14)
S355 Phosphorylation Q16539 (MAPK14) , Q13164 (MAPK7)
K403 Acetylation
K403 Sumoylation
S408 Phosphorylation Q16539 (MAPK14) , Q00535 (CDK5)
S453 Phosphorylation Q16539 (MAPK14)
S457 Phosphorylation
S479 Phosphorylation Q16539 (MAPK14)
S494 Phosphorylation Q16539 (MAPK14)
S496 Phosphorylation

研究背景

功能:

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation. Associates with chromatin to the ZNF16 promoter.

翻译修饰:

Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.

Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated with SUMO1 and, to a lesser extent with SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis (By similarity).

Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.

Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.

亚基结构:

Binds DNA as a homo- or heterodimer. Dimerizes with MEF2D. Interacts with HDAC7 (By similarity). Interacts with PIAS1; the interaction enhances sumoylation. Interacts with HDAC4, HDAC9 and SLC2A4RG. Interacts (via the N-terminal) with MAPK7; the interaction results in the phosphorylation and transcriptional activity of MEF2A.

蛋白家族:

Belongs to the MEF2 family.

研究领域

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

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