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| 产品: | TEX10 抗体 |
| 货号: | DF12486 |
| 描述: | Rabbit polyclonal antibody to TEX10 |
| 应用: | WB IHC |
| 文献验证: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 蛋白号: | Q9NXF1 |
| RRID: | AB_2845291 |
产品描述
来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
TEX10 Antibody detects endogenous levels of total TEX10.
RRID:
AB_2845291
引用格式: Affinity Biosciences Cat# DF12486, RRID:AB_2845291.
引用格式: Affinity Biosciences Cat# DF12486, RRID:AB_2845291.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
抗原和靶标
免疫原:
A synthesized peptide derived from human TEX10, corresponding to a region within the internal amino acids.
基因/基因ID:
文献引用
1). RNAi Screening Identifies that TEX10 Promotes the Proliferation of Colorectal Cancer Cells by Increasing NF-κB Activation. Advanced Science, 2020
(PubMed: 32995120)
[IF=15.1]
Application: WB Species: Human Sample: CRC tissues
Figure 1 Screening indicates that TEX10 is upregulated and is associated with prognosis in CRC. A) Schematic of the shRNA library screening strategy for genes that affected CRC cell proliferation. B) Schematic of the overlapping results of shRNA‐screened genes and differentially expressed genes in tumors. C) Heatmap of the overlapping genes in (B). D) TEX10 expression profile in various tumor types and normal tissues as identified through the GEPIA website. E) qPCR analysis of TEX10 expression in 16 CRC tissues and adjacent normal mucosal tissues. F) IB analysis of TEX10 protein expression in paired CRC tissues. N, normal tissues; T, tumor tissues. G) IHC scores of TEX10 in 129 paired CRC and adjacent normal tissues. H) Kaplan–Meier analysis of overall survival and I) progression‐free survival according to TEX10 levels in CRC patients. *P < 0.05, **P < 0.01, and ***P < 0.001 (E,G) two‐tailed Student's t‐test or H,I) log‐rank test).
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