产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12471, RRID:AB_2845276.
展开/折叠
ERdj2; PRO2507; SEC 63; SEC63L; Translocation protein SEC63 homolog;
抗原和靶标
- Q9UGP8 SEC63_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGQQFQYDDSGNTFFYFLTSFVGLIVIPATYYLWPRDQNAEQIRLKNIRKVYGRCMWYRLRLLKPQPNIIPTVKKIVLLAGWALFLFLAYKVSKTDREYQEYNPYEVLNLDPGATVAEIKKQYRLLSLKYHPDKGGDEVMFMRIAKAYAALTDEESRKNWEEFGNPDGPQATSFGIALPAWIVDQKNSILVLLVYGLAFMVILPVVVGSWWYRSIRYSGDQILIRTTQIYTYFVYKTRNMDMKRLIMVLAGASEFDPQYNKDATSRPTDNILIPQLIREIGSINLKKNEPPLTCPYSLKARVLLLSHLARMKIPETLEEDQQFMLKKCPALLQEMVNVICQLIVMARNREEREFRAPTLASLENCMKLSQMAVQGLQQFKSPLLQLPHIEEDNLRRVSNHKKYKIKTIQDLVSLKESDRHTLLHFLEDEKYEEVMAVLGSFPYVTMDIKSQVLDDEDSNNITVGSLVTVLVKLTRQTMAEVFEKEQSICAAEEQPAEDGQGETNKNRTKGGWQQKSKGPKKTAKSKKKKPLKKKPTPVLLPQSKQQKQKQANGVVGNEAAVKEDEEEVSDKGSDSEEEETNRDSQSEKDDGSDRDSDREQDEKQNKDDEAEWQELQQSIQRKERALLETKSKITHPVYSLYFPEEKQEWWWLYIADRKEQTLISMPYHVCTLKDTEEVELKFPAPGKPGNYQYTVFLRSDSYMGLDQIKPLKLEVHEAKPVPENHPQWDTAIEGDEDQEDSEGFEDSFEEEEEEEEDDD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UGP8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y106 | Phosphorylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K135 | Ubiquitination | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| Y149 | Phosphorylation | Uniprot | |
| T153 | Phosphorylation | Uniprot | |
| S157 | Phosphorylation | Uniprot | |
| T269 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| K313 | Ubiquitination | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| K402 | Methylation | Uniprot | |
| K403 | Methylation | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| S451 | Phosphorylation | Uniprot | |
| T475 | Phosphorylation | Uniprot | |
| T478 | Phosphorylation | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| K506 | Ubiquitination | Uniprot | |
| K510 | Ubiquitination | Uniprot | |
| K527 | Acetylation | Uniprot | |
| T537 | Phosphorylation | Uniprot | |
| S544 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S574 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| S593 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| K607 | Ubiquitination | Uniprot | |
| K623 | Ubiquitination | Uniprot | |
| K631 | Ubiquitination | Uniprot | |
| K633 | Ubiquitination | Uniprot | |
| Y642 | Phosphorylation | Uniprot | |
| K688 | Ubiquitination | Uniprot | |
| Y692 | Phosphorylation | Uniprot | |
| Y694 | Phosphorylation | Uniprot | |
| T695 | Phosphorylation | Uniprot | |
| K710 | Ubiquitination | Uniprot | |
| S742 | Phosphorylation | Uniprot | |
| S748 | Phosphorylation | Uniprot |
研究背景
Mediates cotranslational and post-translational transport of certain precursor polypeptides across endoplasmic reticulum (ER). Proposed to play an auxiliary role in recognition of precursors with short and apolar signal peptides. May cooperate with SEC62 and HSPA5/BiP to facilitate targeting of small presecretory proteins into the SEC61 channel-forming translocon complex, triggering channel opening for polypeptide translocation to the ER lumen. Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia (By similarity).
Endoplasmic reticulum membrane>Multi-pass membrane protein.
Widely expressed, with high levels in the liver.
The ER translocon complex consists of channel-forming core components SEC61A1, SEC61B and SEC61G and different auxiliary components such as SEC62 and SEC63.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein export.
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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