OMA1 Antibody - #DF12435

Figure 4. NLRX1 induces selective mitochondrial fission and mitophagy to maintain adaptive mitochondrial morphology. Primary human NP cells isolated from degenerated NP tissues were prepared. (A) Co-localization analysis of mCherry and GFP in live stable mCherry-GFP-MAP1LC3B-expressing NP cells following the treatments of PBS, TBHP or Baf-A1 with NLRX1 overexpression or not, scale bar: 200 μm. (B and C) protein expressions of mitophagy indicators (MAP1LC3B-II, TOMM20, TIMM23) in primary human NP cells isolated from degenerated NP tissues following the treatments of PBS, TBHP or Baf-A1 with NLRX1 overexpression or not, as determined by western blotting. (D-F) mitochondrial morphology analysis by fluorescence microscope with MitoTracker Red CMXRos label (D and E) and transmission electron microscopy (TEM) (F) in primary human NP cells isolated from degenerated NP tissues following the treatments of PBS or TBHP with NLRX1 overexpression or not, fluorescent scale bar: 5 μm, TEM scale bar: 2 μm (upper panel), 500 nm (lower panel). (G and H) protein expressions of mitochondrial dynamics indicators (p-DNM1L, DNM1L, MFF, MFN1, MFN2, OPA1, OMA1) in primary human NP cells isolated from degenerated NP tissues following the treatments of PBS or TBHP with NLRX1 overexpression or not. (I and J) protein expressions of PINK1 and PRKN in primary human NP cells isolated from degenerated NP tissues following the treatments of PBS or TBHP with NLRX1 overexpression or not. (K) confocal analysis of MitoTracker labeling and PRKN protein with if staining in primary human NP cells isolated from degenerated NP tissues following the treatments of PBS or TBHP with NLRX1 overexpression or not, scale bar: 10 μm. Data are represented as mean ± SD.

Fig. 5. LCN2 regulates OMA1 and OPA1, thereby disrupting the formation of mitochondrial donuts and osteogenesis. A Volcano plot showing significant DEPs (fold change > 1.5 or