| 产品: | NCKAP1 抗体 |
| 货号: | DF12428 |
| 描述: | Rabbit polyclonal antibody to NCKAP1 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 115-125 kDa; 129kD(Calculated). |
| 蛋白号: | Q9Y2A7 |
| RRID: | AB_2845233 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12428, RRID:AB_2845233.
展开/折叠
FLJ11291; KIAA0587; Membrane associated protein HEM2; Membrane-associated protein HEM-2; MGC8981; NAP 1; NAP1; NAP125; NCK associated protein 1; NCK associated protein; Nck-associated protein 1; Nckap1; NCKP1_HUMAN; OTTHUMP00000163460; OTTHUMP00000163461; p125Nap1;
抗原和靶标
Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.
- Q9Y2A7 NCKP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y2A7 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| T18 | Phosphorylation | Uniprot | |
| R23 | Methylation | Uniprot | |
| T29 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| T142 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| Y175 | Phosphorylation | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| K201 | Acetylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| K551 | Ubiquitination | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| S638 | Phosphorylation | Uniprot | |
| K643 | Ubiquitination | Uniprot | |
| K644 | Ubiquitination | Uniprot | |
| S645 | Phosphorylation | Uniprot | |
| K651 | Ubiquitination | Uniprot | |
| S664 | Phosphorylation | Uniprot | |
| Y691 | Phosphorylation | Uniprot | |
| S719 | Phosphorylation | Uniprot | |
| S801 | Phosphorylation | Uniprot | |
| S838 | Phosphorylation | Uniprot | |
| S840 | Phosphorylation | Uniprot | |
| S852 | Phosphorylation | Uniprot | |
| K886 | Ubiquitination | Uniprot | |
| K895 | Ubiquitination | Uniprot | |
| S899 | Phosphorylation | Uniprot | |
| S902 | Phosphorylation | Uniprot | |
| T908 | Phosphorylation | Uniprot | |
| T1045 | Phosphorylation | Uniprot | |
| S1050 | Phosphorylation | Uniprot | |
| S1065 | Phosphorylation | Uniprot | |
| K1075 | Ubiquitination | Uniprot | |
| Y1116 | Phosphorylation | Uniprot | |
| Y1120 | Phosphorylation | Uniprot | |
| S1123 | Phosphorylation | Uniprot |
研究背景
Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.
Cell membrane>Single-pass membrane protein>Cytoplasmic side. Cell projection>Lamellipodium membrane>Single-pass membrane protein>Cytoplasmic side.
Note: At the interface between the lamellipodial actin meshwork and the membrane.
Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.
Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Associates preferentially with the first SH3 domain of NCK. Interacts with NYAP1, NYAP2 and MYO16 (By similarity).
(Microbial infection) Interacts with human cytomegalovirus protein UL135.
Belongs to the HEM-1/HEM-2 family.
研究领域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
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