HPSE Antibody - #DF12411

Fig. 2. GTS-21 regulated the ACE/ACE2 ratio and glycocalyx shedding in LPS-ALI mice A-D ELISA kits were used to quantify the concentrations of major renin-angiotensin system (RAS) regulating enzymes (ACE, Ang II, ACE2, and Ang 1-7) in the lung tissue (n = 6 per group). E Protein levels of important RAS-regulating enzymes (ACE, AT1R, ACE2, and MasR) (n = 3 per group). The relative protein levels are displayed. F Levels of NF-κB phosphoproteins (p-P65 and p-IκB) (n = 3 per group). The relative protein levels are displayed. The immunofluorescence of HS, SDC-1, and surfactant protein D in the lungs is shown in G in representative images and densitometry (scar bar, 20 mm, n = 3 per group). H Evans Blue staining tested lung permeability (n = 3 for each group). Using ELISA, we assessed the BALF concentrations of HS I and HPA J; SP-D in serum K and BALF L (n = 5 for each group). M Relative protein levels of SDC-1, HPA, EXT-1, and SP-D in The lung tissues (n = 3 for each group). All data were shown as mean ± SD.

Figure 2 AMPK regulates the expression of Hpa-1 and TJ proteins. (A) Western blot analysis of Hpa-1, Sdc-1, occludin-1 and ZO-1 expression. Densitometric analysis of (B) Hpa-1 and (C) Sdc-1 expression. (D) Expression levels of Sdc-1 in the cell culture supernatant. (E) Densitometric analysis of occludin-1 and (F) ZO-1 expression. Data are presented as the mean ± standard deviation of three repeats. *P<0.05, **P<0.01, ***P<0.001 vs. the control group; #P<0.05, ##P<0.01 vs. HG. AMPK, adenosine monophosphate-activated protein kinase; Hpa-1, heparanase-1; TJ, tight junction; Sdc-1, syndecan-1; ZO-1, Zonula occludens-1; HG, high glucose.

Figure 5 PF alleviates endothelial glycocalyx injury in vivo. (A) Transmission electron microscopy of glomeruli extracted from different groups of animals. (B) The number of glomerular endothelial fenestrations. (C) FITC-WGA staining for endothelial glycocalyx in the glomerulus. Magnification, x400. (D) Quantification analysis of FITC-WGA staining. (E) Immunohistochemical images of p-AMPKα (Thr-172). Magnification, x400. (F) Quantification analysis of immunohistochemical staining of p-AMPKα (Thr-172). (G) Immunohistochemical staining of Hpa-1. Magnification, x400. (H) Quantification analysis of immunohistochemical staining of Hpa-1. Data are presented as the mean ± standard deviation of three repeats. **P<0.01, ***P<0.01 vs. the control group; #P<0.05, ##P<0.01 vs. the model group. PF, piperazine ferulate; FITC, fluorescein isothiocyanate; WGA, wheat germ agglutinin; p-, phosphorylated-; AMPK, adenosine monophosphate-activated protein kinase; Hpa-1, heparanase-1.