GSTM3 Antibody - #DF12408

(2)   (2)  
产品: GSTM3 抗体
货号: DF12408
描述: Rabbit polyclonal antibody to GSTM3
应用: WB IHC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 27-29 kDa; 27kD(Calculated).
蛋白号: P21266
RRID: AB_2845213

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(80%), Dog(90%)
克隆:
Polyclonal
特异性:
GSTM3 Antibody detects endogenous levels of total GSTM3.
RRID:
AB_2845213
引用格式: Affinity Biosciences Cat# DF12408, RRID:AB_2845213.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

brain GST; brain type mu glutathione S transferase; glutathione S alkyltransferase M3; glutathione S aryltransferase M3; glutathione S transferase M3 (brain); glutathione S transferase M3; Glutathione S transferase Mu 3; glutathione S transferase, Mu 3; Glutathione S-transferase Mu 3; GST class mu 3; GST class-mu 3; GST5; GSTB; Gstm3; GSTM3-3; GSTM3_HUMAN; GTM3; hGSTM3-3; MGC3310; MGC3704; S (hydroxyalkyl)glutathione lyase M3;

抗原和靶标

免疫原:
Uniprot:

P21266(GSTM3_HUMAN) >>Visit HPA database.

基因/基因ID:
GSTM3(2947)
表达:
P21266 GSTM3_HUMAN:

Testis and brain.

序列:
MSCESSMVLGYWDIRGLAHAIRLLLEFTDTSYEEKRYTCGEAPDYDRSQWLDVKFKLDLDFPNLPYLLDGKNKITQSNAILRYIARKHNMCGETEEEKIRVDIIENQVMDFRTQLIRLCYSSDHEKLKPQYLEELPGQLKQFSMFLGKFSWFAGEKLTFVDFLTYDILDQNRIFDPKCLDEFPNLKAFMCRFEALEKIAAYLQSDQFCKMPINNKMAQWGNKPVC

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
90
Bovine
90
Sheep
90
Dog
90
Rabbit
80
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P21266 作为底物

Site PTM Type Enzyme
S2 Phosphorylation
T28 Phosphorylation
T30 Phosphorylation
S31 Phosphorylation
K35 Ubiquitination
C39 S-Nitrosylation
R47 Methylation
K54 Sumoylation
K54 Ubiquitination
K56 Ubiquitination
Y66 Phosphorylation
K71 Acetylation
K71 Ubiquitination
K73 Ubiquitination
T75 Phosphorylation
R82 Methylation
K87 Ubiquitination
S122 Phosphorylation
K128 Sumoylation
K128 Ubiquitination
K140 Ubiquitination
S143 Phosphorylation
K177 Ubiquitination
K186 Ubiquitination
K197 Ubiquitination
S204 Phosphorylation
C208 S-Nitrosylation
K209 Ubiquitination
K215 Ubiquitination
K222 Ubiquitination

研究背景

功能:

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.

翻译修饰:

The N-terminus is blocked.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Testis and brain.

亚基结构:

Homodimer.

蛋白家族:

Belongs to the GST superfamily. Mu family.

研究领域

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

文献引用

1). Cardioprotective role of A-cycloglycosylated derivative of Rubiadin in diabetic cardiomyopathy in rats. International Immunopharmacology (PubMed: 36989899) [IF=5.6]
2). Propofol inhibits proliferation and migration of glioma cells by up-regulating lncRNA GAS5. TOXICOLOGY IN VITRO (PubMed: 35085768) [IF=3.2]

Application: WB    Species: human    Sample: U251 cells

Fig. 5. |Propofol regulates the expression of c-Myc, P21 and GSTM3. (A) After transfected by siRNAs and treated with 8 μg/ml propofol for 6 h, Western blot analysis was conducted to measure effects of propofol on protein expression of c-Myc, P21 and GSTM3 in U251 cells.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.