CX3CL1 Antibody - #DF12376

Figure 12 CX3CL1 Knockdown promoted migration and invasion of ccRCC cell in vitro. (A) Real-time RT-PCR assay of 10 LR-pairs mRNA expression levels in human ccRCC cell line 786-O and renal epithelial cell HK-2. (B, C) the protein expression of CX3CL1 was performed by Western Blot assay. (D) The subcellular localization of CX3CL1 was identified by immunostaining assay. Scale bar: 10μm. (E) Cell colonies of 786-O cell in normal control and CX3CL1-KD group. (F, G) 786-O cell migration assay (F) and invasion assay (G) were performed in normal control and CX3CL1-KD group. ns, p≥0.05; *p< 0.05; **p<0.01; ***p<0.001.

Figure 12 CX3CL1 Knockdown promoted migration and invasion of ccRCC cell in vitro. (A) Real-time RT-PCR assay of 10 LR-pairs mRNA expression levels in human ccRCC cell line 786-O and renal epithelial cell HK-2. (B, C) the protein expression of CX3CL1 was performed by Western Blot assay. (D) The subcellular localization of CX3CL1 was identified by immunostaining assay. Scale bar: 10μm. (E) Cell colonies of 786-O cell in normal control and CX3CL1-KD group. (F, G) 786-O cell migration assay (F) and invasion assay (G) were performed in normal control and CX3CL1-KD group. ns, p≥0.05; *p< 0.05; **p<0.01; ***p<0.001.

Figure 12 CX3CL1 Knockdown promoted migration and invasion of ccRCC cell in vitro. (A) Real-time RT-PCR assay of 10 LR-pairs mRNA expression levels in human ccRCC cell line 786-O and renal epithelial cell HK-2. (B, C) the protein expression of CX3CL1 was performed by Western Blot assay. (D) The subcellular localization of CX3CL1 was identified by immunostaining assay. Scale bar: 10μm. (E) Cell colonies of 786-O cell in normal control and CX3CL1-KD group. (F, G) 786-O cell migration assay (F) and invasion assay (G) were performed in normal control and CX3CL1-KD group. ns, p≥0.05; *p< 0.05; **p<0.01; ***p<0.001.

FIGURE 1 FKN positively interacted with Wnt/β- catenin signalling pathway in J774A.1 cells. A, Expression profile of FKN- regulated genes using the RNA- seq. B, Differentially expressed genes are shown in a volcano map. C, Biological processes were revealed in RNA- sequencing. D, The data sets were analysed by GESA using the Hallmark collection. The correlation between FKN and Wnt/β- catenin signalling was indicated in GESA. E- G, Co- IP and IF assays detected the interaction between FKN and Wnt/β- catenin signalling and this was visualized using confocal microscopy with specific antibodies (green). Nuclei were incubated with DAPI (blue). The cytoskeletons were incubated with β- tubulin (red). Scale bars represent 10 μm

FIGURE 1 FKN positively interacted with Wnt/β- catenin signalling pathway in J774A.1 cells. A, Expression profile of FKN- regulated genes using the RNA- seq. B, Differentially expressed genes are shown in a volcano map. C, Biological processes were revealed in RNA- sequencing. D, The data sets were analysed by GESA using the Hallmark collection. The correlation between FKN and Wnt/β- catenin signalling was indicated in GESA. E- G, Co- IP and IF assays detected the interaction between FKN and Wnt/β- catenin signalling and this was visualized using confocal microscopy with specific antibodies (green). Nuclei were incubated with DAPI (blue). The cytoskeletons were incubated with β- tubulin (red). Scale bars represent 10 μm

FIGURE 1 |FKN positively interacted with Wnt/β-catenin signalling pathway in J774A.1 cells. E-G, Co-IP and IF assays detected the interaction between FKN and Wnt/β-catenin signalling and this was visualized using confocal microscopy with specific antibodies (green). Nuclei were incubated with DAPI (blue). The cytoskeletons were incubated with β-tubulin (red). Scale bars represent 10 μm

Fig. 4. |Hypoxia promotes platelet recruitment by upregulating CX3CL1 expression.(C) Expression of CX3CL1 and CA9 in human HCC and NT liver tissues. Representative images of two consecutive slices of the same sample embedded in paraffin were taken, and each was stained with CX3CL1 and CA9 (n = 3). Bar, 200 lm.

Figure 2. mRNA and protein expression of CX3CL1 and CX3CR1 in OSCC cell lines. Notes: (A) and (B) show the mRNA expression levels of CX3CL1 and CX3CR1 in HOK and various OSCC cell lines using PCR; (C) and (D) show the protein expression levels of membrane-bound CX3CL1 and CX3CR1 in HOK and various OSCC cell lines using WB, along with their corresponding gray value analysis; (E) shows the OD value analysis of soluble CX3CL1 protein expression in HOK and various OSCC cell lines using ELISA. The results indicate that CX3CL1 mRNA and protein expression levels are significantly decreased in most OSCC cell lines compared to HOK, while soluble CX3CL1 that has the function of chemoattracting cells expressing CX3CR1. Expression is increased and CX3CR1 protein content is significantly decrease. Data were shown as mean with SD (n ≥ 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns p > 0.05. The inconsistent results between PCR and WB may be due to differences in transcription and translation control. The low expression levels of CX3CL1 and CX3CR1 genes in OSCC, as validated by mRNA and protein content analysis in OSCC tissues and cell lines, suggest that they may play a suppressive role in the development and progression of OSCC.

Fig. 7. FKN depletion attenuates apoptosis of Treg cells derived from lupus model mice. (a) Treg cell apoptosis detected by flow cytometric analysis. (b) Dot plot showing quantified levels of apoptotic cells (Q2 and Q3). Bax, Bcl-2, and Cyt-c protein and mRNA levels were detected by (c, d) Western blot and (e) qRT-PCR, respectively. #p < 0.05 vs. control; #$p < 0.05 vs. SB203580; #&p > 0.05 vs. FKN KD; *p > 0.05 vs. control; *$ P > 0.05 vs. control. Data represent the mean ± SD of three independent experiments performed in triplicate.