产品: Aconitase 2 抗体
货号: DF12346
描述: Rabbit polyclonal antibody to Aconitase 2
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 85 kDa; 85kD(Calculated).
蛋白号: Q99798
RRID: AB_2845151

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(91%), Chicken(100%), Xenopus(90%)
克隆:
Polyclonal
特异性:
Aconitase 2 Antibody detects endogenous levels of total Aconitase 2.
RRID:
AB_2845151
引用格式: Affinity Biosciences Cat# DF12346, RRID:AB_2845151.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ACO 2; ACO2; ACON_HUMAN; aconitase 2; Aconitase 2 mitochondrial; Aconitase; Aconitase2; Aconitate hydratase; Aconitate hydratase mitochondrial; ACONM; Citrate hydro lyase; Citrate hydro-lyase; ICRD; mitochondrial;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MAPYSLLVTRLQKALGVRQYHVASVLCQRAKVAMSHFEPNEYIHYDLLEKNINIVRKRLNRPLTLSEKIVYGHLDDPASQEIERGKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPSTIHCDHLIEAQVGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWKPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWSSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGREDIANLADEFKDHLVPDPGCHYDQLIEINLSELKPHINGPFTPDLAHPVAEVGKVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQILRDLGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDYLTGTDGKKFRLEAPDADELPKGEFDPGQDTYQHPPKDSSGQHVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHPVDKLTIQGLKDFTPGKPLKCIIKHPNGTQETILLNHTFNETQIEWFRAGSALNRMKELQQ

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Chicken
100
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Xenopus
90
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q99798 作为底物

Site PTM Type Enzyme
T9 Phosphorylation
Y42 Phosphorylation
K50 Acetylation
Y71 Phosphorylation P09769 (FGR)
S79 Phosphorylation
S87 Phosphorylation
K144 Acetylation
K144 Ubiquitination
Y151 Phosphorylation
K233 Acetylation
S237 Phosphorylation
S242 Phosphorylation
S243 Phosphorylation
K245 Ubiquitination
K309 Ubiquitination
T310 Phosphorylation
K370 Acetylation
K370 Ubiquitination
Y390 Phosphorylation
K401 Ubiquitination
K409 Ubiquitination
C410 S-Nitrosylation
K411 Ubiquitination
Y432 Phosphorylation
T467 Phosphorylation
T470 Phosphorylation
S471 Phosphorylation
R474 Methylation
T477 Phosphorylation
Y513 Phosphorylation
T515 Phosphorylation
K520 Acetylation
K520 Ubiquitination
K521 Acetylation
R523 Methylation
K534 Acetylation
K534 Ubiquitination
T543 Phosphorylation
Y544 Phosphorylation P09769 (FGR)
K549 Acetylation
S559 Phosphorylation
K573 Acetylation
K573 Ubiquitination
K577 Acetylation
C592 S-Nitrosylation
K605 Acetylation
K605 Ubiquitination
T646 Phosphorylation
Y665 Phosphorylation P09769 (FGR)
S669 Phosphorylation
S670 Phosphorylation
R679 Methylation
K689 Acetylation
K689 Ubiquitination
K700 Acetylation
K701 Ubiquitination
K723 Ubiquitination
K730 Acetylation
K730 Ubiquitination
K743 Acetylation

研究背景

功能:

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

细胞定位:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Monomer.

蛋白家族:

Belongs to the aconitase/IPM isomerase family.

研究领域

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

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