Src Antibody - #AF6161

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产品: Src 抗体
货号: AF6161
描述: Rabbit polyclonal antibody to Src
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat, Monkey
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
蛋白号: P12931
RRID: AB_2834796

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat, Monkey
克隆:
Polyclonal
特异性:
Src Antibody detects endogenous levels of total Src.
RRID:
AB_2834796
引用格式: Affinity Biosciences Cat# AF6161, RRID:AB_2834796.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ASV; Avian sarcoma virus; c SRC; CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA; cSrc; EC 2.7.10.2; Neuronal CSRC tyrosine specific protein kinase; Neuronal SRC; Oncogene SRC; OTTHUMP00000174476; OTTHUMP00000174477; p60 Src; p60-Src; p60Src; pp60c src; pp60c-src; pp60csrc; Proto oncogene tyrosine protein kinase Src; Proto-oncogene c-Src; Proto-oncogene tyrosine-protein kinase Src; Protooncogene SRC; Protooncogene SRC Rous sarcoma; Src; SRC Oncogene; SRC proto oncogene non receptor tyrosine kinase; SRC_HUMAN; SRC1; Tyrosine kinase pp60c src; Tyrosine protein kinase SRC 1; Tyrosine protein kinase SRC1; v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog; V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian); v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian;

抗原和靶标

免疫原:

A synthesized peptide derived from human Src, corresponding to a region within C-terminal amino acids.

基因/基因ID:
SRC(6714)
描述:
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Hepatitis B.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Bladder cancer.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Nervous system > GABAergic synapse.

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

· Organismal Systems > Endocrine system > Relaxin signaling pathway.

文献引用

1). ITGB6 modulates resistance to anti-CD276 therapy in head and neck cancer by promoting PF4+ macrophage infiltration. Nature communications, 2024 (PubMed: 39152118) [IF=16.6]
2). Flavonoids from Hippophae rhamnoides Linn. Revert Doxorubicin-Induced Cardiotoxicity through Inhibition of Mitochondrial Dysfunction in H9c2 Cardiomyoblasts In Vitro. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2023 (PubMed: 36834585) [IF=5.6]
3). Increasing the Hindgut Carbohydrate/Protein Ratio by Cecal Infusion of Corn Starch or Casein Hydrolysate Drives Gut Microbiota-Related Bile Acid Metabolism To Stimulate Colonic Barrier Function. mSystems, 2020 (PubMed: 32487741) [IF=5.0]

Application: WB    Species: Human    Sample: Colon

FIG 5 (A to C) Effects of selected bile acid on barrier function (A)-, bile acid receptor (B)-, and epithelial barrier function regulation (C)-related gene expression of Caco-2 cells (n  3). (D) Effects of selected bile acid on the relative protein levels of ZO-1, Src, and EGFR (n  3). GAPDH was used as an internal control. (E) Effects of selected bile acid and EGFR-specific inhibitor (AG-1478) on the relative protein levels of ZO-1, Src, and EGFR (n  3). GAPDH was used as an internal control. (F) Effects of selected bile acid and Src family kinase inhibitor (PP-2) on the relative protein levels of ZO-1 and Src (n  3). GAPDH was used as an internal control. Data are presented as mean  SEM. *, compared with control, P  0.05; **, compared with control, P  0.01; ***, compared with control, P  0.001; , compared with DCA group, P  0.05; , compared with LCA group, P  0.05. CA, cholic acid; DCA, deoxycholic acid; LCA, lithocholic acid; OCLD, occludin; ZO-1, zonula occludens 1; FXR, farnesoid X receptor; TGR5, G-protein-coupled receptor; EGFR, epidermal growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PI3K, phosphatidylinositol 3-kinase; RHOA, ras homolog gene family, member A; MLCK, myosin light chain kinase.
4). Jinfukang regulates integrin/Src pathway and anoikis mediating circulating lung cancer cells migration. JOURNAL OF ETHNOPHARMACOLOGY, 2021 (PubMed: 33068649) [IF=4.8]

Application: WB    Species: human    Sample: CTC-TJH-01 cells

Fig. 5. |Jinfukang inhibited the cell migration via Integrin/Src axis in lung cancer cells. Cells were incubated with Jinfukang (100 μg/mL), ATN-161 (10 μmol/L) or a combination of both for 24 h. A Western blot assay was used to measure the protein expression levels of Integrin β1, Src, and p-Src in CTC-TJH-01 (A) and H1975 (B)cells. GAPDH was used as an internal standard.
5). Albumin Fusion at the N-Terminus or C-Terminus of HM-3 Leads to Improved Pharmacokinetics and Bioactivities. Biomedicines, 2021 (PubMed: 34572270) [IF=4.7]

Application: WB    Species: Mouse    Sample: B16F10 cells

Figure 7 HM-3/HSA inhibited FAK/Src signaling pathways by binding with integrin αvβ3 and α5β1. (A,B) The integrin monoclonal antibody-blocking assay was performed to verify the interaction between HM-3/HSA and both integrin α5β1 and αvβ3. The high binding rate of HM-3/HSA increased with B16F10 cells through binding with integrin αvβ3 and α5β1 of the cell surface, but the adhesion number was significantly decreased when B16F10 cells were blocked with the integrin αvβ3 or α5β1 antibody. Control: cells treated with the PBS vehicle only. Scale bar = 100 μm. Data shown are means ± standard deviation from three independent experiments. ** p < 0.01, *** p < 0.001, and # p < 0.05. (C) HM-3-HSA affected the FAK/Src signaling pathway in B16F10 cells. The B16F10 cells were treated with 0.25 μM HSA-HM-3 or 0.25 μM HM-3-HSA for 48 h. GAPDH was used as the internal reference. Data shown are means ± standard deviation from two independent experiments.
6). HM-3-HSA exhibits potent anti-angiogenesis and antitumor activity in hepatocellular carcinoma. European Journal of Pharmaceutical Sciences, 2021 (PubMed: 34555448) [IF=4.3]
7). Ethoxy-erianin phosphate and afatinib synergistically inhibit liver tumor growth and angiogenesis via regulating VEGF and EGFR signaling pathways. Toxicology and Applied Pharmacology, 2022 (PubMed: 35143806) [IF=3.3]
8). Anti-metastatic Potential of Natural Triterpenoid Cucurbitacin B Against Cholangiocarcinoma Cells by Targeting Src Protein. INTEGRATIVE CANCER THERAPIES, 2022 (PubMed: 36154723) [IF=2.9]
9). Inorganic arsenic exposure promotes malignant progression by HDAC6-mediated down-regulation of HTRA1. Journal of Applied Toxicology, 2023 (PubMed: 36861143) [IF=2.7]

Application: WB    Species: Human    Sample: Caco-2 cells

FIGURE 3 Molecular profiling for iAs-induced changes in Caco-2 cells. (A) Protein levels of claudin 3 and cofilin were determined by Western blot analysis. (B) Transcript levels of IL-6, TNF-α, and MMP13 were determined by RT-qPCR, with GAPDH as an internal control. (C) The levels of EGF in the culture supernatant were examined by ELISA. (D) A marked increase in the phosphorylated levels of EGFR, Src, AKT, and p38 was shown by Western blot analysis. RT-qPCR (E) and Western blot analysis (F) results showed the down-regulation of HTRA1 expression caused by chronic iAs exposure. Data were representatives of at least three independent experiments, shown as mean ± SD. The significance threshold for Student's t-test:
10). Effects of Cinobufagin on the Proliferation, Migration, and Invasion of H1299 Lung Cancer Cells. Chemistry & Biodiversity, 2023 (PubMed: 36522286) [IF=2.3]
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