MDH1 Antibody - #DF12291

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产品: MDH1 抗体
货号: DF12291
描述: Rabbit polyclonal antibody to MDH1
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 36 kDa; 36kD(Calculated).
蛋白号: P40925
RRID: AB_2845096

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 50ul RMB¥ 1250 现货
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实验方案
WB Handbook
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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(92%), Xenopus(83%)
克隆:
Polyclonal
特异性:
MDH1 Antibody detects endogenous levels of total MDH1.
RRID:
AB_2845096
引用格式: Affinity Biosciences Cat# DF12291, RRID:AB_2845096.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

cytoplasmic; Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase; Malate dehydrogenase 1, NAD (soluble); Malate dehydrogenase; Malate dehydrogenase cytoplasmic; MDH s; mdh1; MDHA; MDHC_HUMAN; MDHs; MGC:1375; MOR2; Soluble malate dehydrogenase;

抗原和靶标

免疫原:
Uniprot:

P40925(MDHC_HUMAN) >>Visit HPA database.

基因/基因ID:
MDH1(4190)
序列:
MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
92
Sheep
92
Dog
92
Chicken
92
Rabbit
92
Xenopus
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P40925 作为底物

Site PTM Type Enzyme
S2 Acetylation
Y22 Phosphorylation
T71 Phosphorylation
K73 Acetylation
K73 Ubiquitination
S89 Phosphorylation
K103 Acetylation
K103 Ubiquitination
K107 Acetylation
K107 Ubiquitination
K110 Methylation
K110 Ubiquitination
S111 Phosphorylation
K118 Acetylation
K118 Ubiquitination
Y119 Phosphorylation
K121 Acetylation
K125 Ubiquitination
C137 S-Nitrosylation
K142 Acetylation
K142 Ubiquitination
K149 Ubiquitination
C154 S-Nitrosylation
K164 Ubiquitination
K170 Ubiquitination
T174 Phosphorylation
K179 Ubiquitination
S188 Phosphorylation
S189 Phosphorylation
T190 Phosphorylation
Y192 Phosphorylation
K199 Ubiquitination
K205 Ubiquitination
Y210 Phosphorylation
K214 Acetylation
K214 Ubiquitination
S217 Phosphorylation
K220 Ubiquitination
R230 Methylation
K236 Ubiquitination
K239 Ubiquitination
S241 Phosphorylation
S242 Phosphorylation
S245 Phosphorylation
T296 Phosphorylation
K298 Acetylation
K298 Ubiquitination
S309 Phosphorylation
K312 Ubiquitination
K318 Ubiquitination
T321 Phosphorylation
K324 Ubiquitination
S326 Phosphorylation
S332 Phosphorylation
S333 Phosphorylation

研究背景

翻译修饰:

ISGylated.

Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Homodimer.

蛋白家族:

Belongs to the LDH/MDH superfamily. MDH type 2 family.

研究领域

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

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