| 产品: | CREST 抗体 |
| 货号: | DF12262 |
| 描述: | Rabbit polyclonal antibody to CREST |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat, Monkey |
| 预测: | Pig, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 50 kDa; 43kD(Calculated). |
| 蛋白号: | O75177 |
| RRID: | AB_2845067 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12262, RRID:AB_2845067.
展开/折叠
Calcium-responsive transactivator; CREST; CREST_HUMAN; KIAA0693; LP2261; MGC26711; MGC78386; sarcoma translocation gene on chromosome18like1; SS18; SS18-like protein 1; SS18L1; SSXT; synovial sarcoma translocation; synovial sarcoma translocation gene on chromosome 18-like 1; SYT homolog 1;
抗原和靶标
- O75177 CREST_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSVAFASARPRGKGEVTQQTIQKMLDENHHLIQCILEYQSKGKTAECTQYQQILHRNLVYLATIADSNQNMQSLLPAPPTQNMNLGPGALTQSGSSQGLHSQGSLSDAISTGLPPSSLLQGQIGNGPSHVSMQQTAPNTLPTTSMSISGPGYSHAGPASQGVPMQGQGTIGNYVSRTNINMQSNPVSMMQQQAATSHYSSAQGGSQHYQGQSSIAMMGQGSQGSSMMGQRPMAPYRPSQQGSSQQYLGQEEYYGEQYSHSQGAAEPMGQQYYPDGHGDYAYQQSSYTEQSYDRSFEESTQHYYEGGNSQYSQQQAGYQQGAAQQQTYSQQQYPSQQSYPGQQQGYGSAQGAPSQYPGYQQGQGQQYGSYRAPQTAPSAQQQRPYGYEQGQYGNYQQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
Transcriptional activator which is required for calcium-dependent dendritic growth and branching in cortical neurons. Recruits CREB-binding protein (CREBBP) to nuclear bodies. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP (By similarity).
Nucleus. Chromosome>Centromere>Kinetochore.
Note: Localizes to nuclear bodies. Colocalizes with SGO1 at kinetochore (By similarity).
Ubiquitous; with lowest levels in spleen.
Homodimer. Dimerization may be necessary for its function in neuronal dendritic development. Interacts (via C-terminus) with CREBBP (via N-terminus), EP300 and SMARCA4/BRG1. Interacts with the nBAF complex. Association with CREBBP facilitates transcription while the association with SMARCA4/BRG1 suppresses CREST-mediated transcription in resting neurons (By similarity).
The MFD (multi-functional domain) domain is involved in transcription transactivation, nuclear body targeting and dimerization.
Belongs to the SS18 family.
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