AGO2 Antibody - #DF12246

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产品: AGO2 抗体
货号: DF12246
描述: Rabbit polyclonal antibody to AGO2
应用: WB IHC
文献验证:
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Xenopus
蛋白号: Q9UKV8
RRID: AB_2845051

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
克隆:
Polyclonal
特异性:
AGO2 Antibody detects endogenous levels of total AGO2.
RRID:
AB_2845051
引用格式: Affinity Biosciences Cat# DF12246, RRID:AB_2845051.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Ago 2; AGO2_HUMAN; Argonaute 2; argonaute 2, RISC catalytic component; Argonaute RISC catalytic component 2; Argonaute2; CTA-204B4.6; dAgo2; eIF 2C 2; eIF-2C 2; eIF2C 2; Eif2c2; Eukaryotic translation initiation factor 2C 2; Eukaryotic translation initiation factor 2C subunit 2; hAgo2; MGC3183; PAZ Piwi domain protein; PPD; Protein argonaute-2; Protein slicer; Q10; Slicer protein;

抗原和靶标

免疫原:

A synthesized peptide derived from human AGO2, corresponding to a region within N-terminal amino acids.

基因/基因ID:
AGO2(27161)

文献引用

1). Silencing of HuR Inhibits Osteosarcoma Cell Epithelial-Mesenchymal Transition via AGO2 in Association With Long Non-Coding RNA XIST. Frontiers in Oncology, 2021 (PubMed: 33816232) [IF=3.5]

Application: IF/ICC    Species: Human    Sample: OS cells

Figure 6 Confocal microscopic analysis of AGO2 expression in OS cells. Representative immunofluorescence analysis results for regulation of AGO2 by HuR and lncRNA XIST. SJSA-1 cells with different treatments were fixed with 1% paraformaldehyde and stained with anti-HuR (red) and anti-AGO2 (green) antibodies. Nuclei of cells appeared as blue because of DAPI nuclear staining. (A) Cells were transfected with an NC siRNA for 48 h. (B) Cells were transfected with a HuR siRNA for 48 h. (C) Cells were transfected with an lncRNA XIST siRNA for 48 h. (D) Cells were co-transfected with both HuR and lncRNA XIST siRNAs for 48 h. Stained cells were examined under a Leica confocal immunofluorescence microscope. Upper panel: original magnification 200x; lower panel, original magnification 630x.
2). Long non‑coding RNA L13Rik promotes high glucose-induced mesangial cell hypertrophy and matrix protein expression by regulating miR-2861/CDKN1B axis. PeerJ, 2023 (PubMed: 37868060) [IF=2.3]

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