产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12231, RRID:AB_2845036.
展开/折叠
KIAA0526; LCB 2; LCB2; LCB2a; Long chain base biosynthesis protein 2; Long chain base biosynthesis protein 2a; Serine palmitoyl CoA transferase 2; Serine palmitoyltransferase 2; Serine palmitoyltransferase long chain base subunit 2; Serine palmitoyltransferase subunit II; Serine-palmitoyl-CoA transferase 2; SPT 2; SPT2; SPTC2_HUMAN; SPTLC 2; Sptlc2;
抗原和靶标
- O15270 SPTC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRPEPGGCCCRRTVRANGCVANGEVRNGYVRSSAAAAAAAAAGQIHHVTQNGGLYKRPFNEAFEETPMLVAVLTYVGYGVLTLFGYLRDFLRYWRIEKCHHATEREEQKDFVSLYQDFENFYTRNLYMRIRDNWNRPICSVPGARVDIMERQSHDYNWSFKYTGNIIKGVINMGSYNYLGFARNTGSCQEAAAKVLEEYGAGVCSTRQEIGNLDKHEELEELVARFLGVEAAMAYGMGFATNSMNIPALVGKGCLILSDELNHASLVLGARLSGATIRIFKHNNMQSLEKLLKDAIVYGQPRTRRPWKKILILVEGIYSMEGSIVRLPEVIALKKKYKAYLYLDEAHSIGALGPTGRGVVEYFGLDPEDVDVMMGTFTKSFGASGGYIGGKKELIDYLRTHSHSAVYATSLSPPVVEQIITSMKCIMGQDGTSLGKECVQQLAENTRYFRRRLKEMGFIIYGNEDSPVVPLMLYMPAKIGAFGREMLKRNIGVVVVGFPATPIIESRARFCLSAAHTKEILDTALKEIDEVGDLLQLKYSRHRLVPLLDRPFDETTYEETED
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O15270 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K161 | Ubiquitination | Uniprot | |
| Y176 | Phosphorylation | Uniprot | |
| Y178 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K281 | Methylation | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| Y298 | Phosphorylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| S384 | Phosphorylation | Uniprot | |
| Y387 | Phosphorylation | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| S402 | Phosphorylation | Uniprot | |
| T409 | Phosphorylation | Uniprot | |
| S412 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| S433 | Phosphorylation | Uniprot | |
| K436 | Ubiquitination | Uniprot | |
| Y474 | Phosphorylation | Uniprot | |
| T501 | Phosphorylation | Uniprot | |
| K538 | Ubiquitination | Uniprot | |
| R550 | Methylation | Uniprot | |
| T555 | Phosphorylation | Uniprot | |
| Y557 | Phosphorylation | Uniprot | |
| T560 | Phosphorylation | Uniprot |
研究背景
Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SPTSSB complex displays a preference for C18-CoA substrate. Plays an important role in de novo sphyngolipid biosynthesis which is crucial for adipogenesis (By similarity).
Endoplasmic reticulum membrane>Single-pass membrane protein.
Widely expressed.
Heterodimer with SPTLC1. Component of the serine palmitoyltransferase (SPT) complex, composed of SPTLC1, either SPTLC2 or SPTLC3, and either SPTSSA or SPTSSB. The composition of the complex will define the substrate specificity.
Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.
研究领域
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Metabolism > Lipid metabolism > Sphingolipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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