产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF12220, RRID:AB_2845025.
展开/折叠
3 PGDH; 3-PGDH; 3-phosphoglycerate dehydrogenase; 3PGDH; D-3-phosphoglycerate dehydrogenase; EC 1.1.1.95; Epididymis secretory protein Li 113; HEL S 113; NLS; NLS1; PDG; PGAD; PGD; PGDH; PGDH3; Phgdh; PHGDHD; Phosphoglycerate dehydrogenase; SERA; SERA_HUMAN;
抗原和靶标
- O43175 SERA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAFANLRKVLISDSLDPCCRKILQDGGLQVVEKQNLSKEELIAELQDCEGLIVRSATKVTADVINAAEKLQVVGRAGTGVDNVDLEAATRKGILVMNTPNGNSLSAAELTCGMIMCLARQIPQATASMKDGKWERKKFMGTELNGKTLGILGLGRIGREVATRMQSFGMKTIGYDPIISPEVSASFGVQQLPLEEIWPLCDFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDMVKGKSLTGVVNAQALTSAFSPHTKPWIGLAEALGTLMRAWAGSPKGTIQVITQGTSLKNAGNCLSPAVIVGLLKEASKQADVNLVNAKLLVKEAGLNVTTSHSPAAPGEQGFGECLLAVALAGAPYQAVGLVQGTTPVLQGLNGAVFRPEVPLRRDLPLLLFRTQTSDPAMLPTMIGLLAEAGVRLLSYQTSLVSDGETWHVMGISSLLPSLEAWKQHVTEAFQFHF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O43175 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R7 | Methylation | Uniprot | |
| K8 | Ubiquitination | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K21 | Sumoylation | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K33 | Acetylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| S55 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| T57 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| K58 | Ubiquitination | Uniprot | |
| T60 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| R75 | Methylation | Uniprot | |
| T78 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| T89 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| T125 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| T147 | Phosphorylation | Uniprot | |
| R236 | Methylation | Uniprot | |
| R247 | Methylation | Uniprot | |
| C281 | S-Nitrosylation | Uniprot | |
| K289 | Acetylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| T353 | Phosphorylation | Uniprot | |
| T358 | Phosphorylation | Uniprot | |
| S362 | Phosphorylation | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| C369 | S-Nitrosylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| K380 | Ubiquitination | Uniprot | |
| K384 | Ubiquitination | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| T480 | Phosphorylation | Uniprot | |
| K522 | Ubiquitination | Uniprot |
研究背景
Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.
Homotetramer.
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.
研究领域
· Metabolism > Amino acid metabolism > Glycine, serine and threonine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
限制条款
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