产品: NOB1 抗体
货号: DF12216
描述: Rabbit polyclonal antibody to NOB1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 47-50 kDa; 47kD(Calculated).
蛋白号: Q9ULX3
RRID: AB_2845021

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(82%)
克隆:
Polyclonal
特异性:
NOB1 Antibody detects endogenous levels of total NOB1.
RRID:
AB_2845021
引用格式: Affinity Biosciences Cat# DF12216, RRID:AB_2845021.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Adenocarcinoma antigen recognized by T lymphocytes 4; ART 4; ART4; MST158; MSTP158; Nin one binding protein; NIN1/RPN12 binding protein 1 homolog (S. cerevisiae); nob1; NOB1_HUMAN; NOB1P; Phosphorylation regulatory protein HP-10; Protein ART-4; Protein ART4; PSMD8 binding protein 1; PSMD8BP1; RNA binding protein NOB1; RNA-binding protein NOB1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9ULX3 NOB1_HUMAN:

Detected in liver, lung, placenta, endothelial cells and spleen.

序列:
MAPVEHVVADAGAFLRHAALQDIGKNIYTIREVVTEIRDKATRRRLAVLPYELRFKEPLPEYVRLVTEFSKKTGDYPSLSATDIQVLALTYQLEAEFVGVSHLKQEPQKVKVSSSIQHPETPLHISGFHLPYKPKPPQETEKGHSACEPENLEFSSFMFWRNPLPNIDHELQELLIDRGEDVPSEEEEEEENGFEDRKDDSDDDGGGWITPSNIKQIQQELEQCDVPEDVRVGCLTTDFAMQNVLLQMGLHVLAVNGMLIREARSYILRCHGCFKTTSDMSRVFCSHCGNKTLKKVSVTVSDDGTLHMHFSRNPKVLNPRGLRYSLPTPKGGKYAINPHLTEDQRFPQLRLSQKARQKTNVFAPDYIAGVSPFVENDISSRSATLQVRDSTLGAGRRRLNPNASRKKFVKKR

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
83
Chicken
83
Xenopus
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9ULX3 作为底物

Site PTM Type Enzyme
K25 Ubiquitination
T35 Phosphorylation
K40 Ubiquitination
T42 Phosphorylation
K56 Ubiquitination
Y62 Phosphorylation
K71 Ubiquitination
K72 Ubiquitination
K111 Ubiquitination
T121 Phosphorylation P24941 (CDK2)
K133 Ubiquitination
K135 Ubiquitination
T140 Phosphorylation
S184 Phosphorylation
S201 Phosphorylation
T210 Phosphorylation
K275 Methylation
K275 Ubiquitination
S286 Phosphorylation
K291 Ubiquitination
T292 Phosphorylation
K315 Ubiquitination
Y324 Phosphorylation
S325 Phosphorylation
T328 Phosphorylation
K330 Ubiquitination
K333 Acetylation
K333 Ubiquitination
Y334 Phosphorylation
T341 Phosphorylation
R345 Methylation
S352 Phosphorylation
K358 Ubiquitination
T359 Phosphorylation
Y366 Phosphorylation
S390 Phosphorylation
R396 Methylation
S404 Phosphorylation

研究背景

功能:

May play a role in mRNA degradation (Probable). Endonuclease required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits (By similarity).

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in liver, lung, placenta, endothelial cells and spleen.

亚基结构:

Interacts with UPF2 (Probable). Component of the small ribosomal subunit, ribosomal RNA processing complex (SSU RRP complex).

蛋白家族:

Belongs to the NOB1 family.

研究领域

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

文献引用

1). Overexpression of microRNA-107 suppressed proliferation, migration, invasion, and the PI3K/Akt signaling pathway and induced apoptosis by targeting Nin one binding (NOB1) protein in a hypopharyngeal squamous cell carcinoma cell line (FaDu). Bioengineered (PubMed: 35294329) [IF=4.9]

Application: WB    Species: Human    Sample: FaDu cells

Figure 6. NOB1 was a target gene of miR-107. (a) MiR-107 target site in the NOB1 3’ UTR WT was predicted. (b) The dual luciferase reporter assay was employed to demonstrate that NOB1 was a target gene of miR-107. (c) FaDu cells were transfected with miR-107 mimics or mimics NC for 48 h, and the protein expression of NOB1 was detected. Data were expressed as means ± SD (n = 3). **P < 0.01 versus the NOB1 3’ UTR MT+miR-107 mimics group. WT, wild type; MT, mutant type.

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