NOB1 Antibody - #DF12216

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产品: NOB1 抗体
货号: DF12216
描述: Rabbit polyclonal antibody to NOB1
应用: WB IHC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 47-50 kDa; 47kD(Calculated).
蛋白号: Q9ULX3
RRID: AB_2845021

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Zebrafish(%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%), Xenopus(%)
克隆:
Polyclonal
特异性:
NOB1 Antibody detects endogenous levels of total NOB1.
RRID:
AB_2845021
引用格式: Affinity Biosciences Cat# DF12216, RRID:AB_2845021.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Adenocarcinoma antigen recognized by T lymphocytes 4; ART 4; ART4; MST158; MSTP158; Nin one binding protein; NIN1/RPN12 binding protein 1 homolog (S. cerevisiae); nob1; NOB1_HUMAN; NOB1P; Phosphorylation regulatory protein HP-10; Protein ART-4; Protein ART4; PSMD8 binding protein 1; PSMD8BP1; RNA binding protein NOB1; RNA-binding protein NOB1;

抗原和靶标

免疫原:
Uniprot:

Q9ULX3(NOB1_HUMAN) >>Visit HPA database.

基因/基因ID:
NOB1(28987)
表达:
Q9ULX3 NOB1_HUMAN:

Detected in liver, lung, placenta, endothelial cells and spleen.

序列:
MAPVEHVVADAGAFLRHAALQDIGKNIYTIREVVTEIRDKATRRRLAVLPYELRFKEPLPEYVRLVTEFSKKTGDYPSLSATDIQVLALTYQLEAEFVGVSHLKQEPQKVKVSSSIQHPETPLHISGFHLPYKPKPPQETEKGHSACEPENLEFSSFMFWRNPLPNIDHELQELLIDRGEDVPSEEEEEEENGFEDRKDDSDDDGGGWITPSNIKQIQQELEQCDVPEDVRVGCLTTDFAMQNVLLQMGLHVLAVNGMLIREARSYILRCHGCFKTTSDMSRVFCSHCGNKTLKKVSVTVSDDGTLHMHFSRNPKVLNPRGLRYSLPTPKGGKYAINPHLTEDQRFPQLRLSQKARQKTNVFAPDYIAGVSPFVENDISSRSATLQVRDSTLGAGRRRLNPNASRKKFVKKR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
83
Chicken
83
Xenopus
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

May play a role in mRNA degradation (Probable). Endonuclease required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits (By similarity).

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in liver, lung, placenta, endothelial cells and spleen.

亚基结构:

Interacts with UPF2 (Probable). Component of the small ribosomal subunit, ribosomal RNA processing complex (SSU RRP complex).

蛋白家族:

Belongs to the NOB1 family.

研究领域

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

文献引用

1). Overexpression of microRNA-107 suppressed proliferation, migration, invasion, and the PI3K/Akt signaling pathway and induced apoptosis by targeting Nin one binding (NOB1) protein in a hypopharyngeal squamous cell carcinoma cell line (FaDu). Bioengineered, 2022 (PubMed: 35294329) [IF=4.2]

Application: WB    Species: Human    Sample: FaDu cells

Figure 6. NOB1 was a target gene of miR-107. (a) MiR-107 target site in the NOB1 3’ UTR WT was predicted. (b) The dual luciferase reporter assay was employed to demonstrate that NOB1 was a target gene of miR-107. (c) FaDu cells were transfected with miR-107 mimics or mimics NC for 48 h, and the protein expression of NOB1 was detected. Data were expressed as means ± SD (n = 3). **P < 0.01 versus the NOB1 3’ UTR MT+miR-107 mimics group. WT, wild type; MT, mutant type.

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